Estimation of genetic divergence among elite mungbean Vigna radiata genotypes by RAPD analysis

Abstract

Genetic diversity among 15 mungbean genotypes of Pakistan was assessed through Random Amplified Polymorphic DNA RAPD analysis, with 30 random decamer primers using polymerase chain reaction PCR . A total of 370 bands were observed, with 12.3 bands per primer, of which 91.6% were polymorphic. OPG-08 produced maximum number of fragments while minimum numbers of fragments were produced with primer OPH-05. Cluster analysis by the Unweighted Paired Group Method of Arithmetic means UPGMA showed that these 15 genotypes could be classified in five groups with a similarity ranging from 0.48-0.86. Maximum similarity was observed between NM-51, NM-54 and NM-98 0.86 . Interestingly, these mungbean genotypes have been developed at one breeding center, while ML-5 was found the least similar line due to its exotic nature. The analysis revealed that the inter-varietal genetic relationship of several genotypes is related to their center of origin. Most of the mungbean genotypes have a narrow genetic base. These results correspond well with previous reported results on mungbean from other countries. The RAPD analysis indicated that it may be a more efficient marker than morphological marker, isozyme and RFLP technology. Based on present results, these genotypes could be successfully utilized in selecting divergent parents for breeding and mapping purposes in future

Keywords

• Cluster Analysis,Mungbean genotypes,Diversity,RAPD,Genetic similarities

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