Seed germination studies on rare endemic Salvia smyrnaea Boiss. Lamiaceae

Abstract

Salvia smyrnaea, is an endemic in Turkey with restricted distribution, which is classified as only two localities known risk category "Endangered En ". In this study, ex-situ conservation purposes of Salvia smyrnaea seed germination requirements were investigated. Study material was collected, in August of 2008, western of Izmir in the Nif Mountain from randomly selected individuals of the population which consists of mature fruits. To determine seed viability, tetrazolium chloride TTC test was performed. Pretreatment was applied 45 days and not applied control seeds glass Petri dishes, at 250 ppm, 500 ppm and 1000 ppm, 2000 ppm different concentrations of 10 ml of gibberellic acid GA3 solution and distilled water control ; at 25 °C 12 hours light L /15 °C, 12 hours dark D alternate temperature and light-dark conditions, were subjected to germination tests. As a result of testing of seed viability, 85% viability in seeds was determined. In 12L/12D light photoperiod not apply pretreatment 40% germination in seeds was detected at 250 ppm the highest, the lowest 6.6% germination in seeds was determined in control seeds. In this study, S. smyrnaea, for ex-situ cultivation of the highest germination percentage, stratification 45 days 5ºC applied and 25/15°C alternate temperature, continuous dark conditions can be achieved with 250 ppm GA3 treated seeds, respectively

Keywords

• Nif Mountain,Salvia smyrnaea,Seed Germination,Endemic

Nadir endemik Salvia smyrnaea Boiss. Lamiaceae tohumlarında çimlendirme çalışmaları

Abstract

Salvia smyrnaea, Türkiye’de yalnızca iki lokaliteden bilinen tehlike kategorisi “Tehlikede En ” olan sınırlı yayılışa sahip bir endemiktir. Bu çalışmada, Salvia smyrnaea’nın ex-situ korunması amacı ile tohum çimlenme gereksinimleri araştırılmıştır. Çalışma materyalini, 2008 yılı ağustos ayında İzmir ilinin batısında bulunan Nif Dağ’ı Kemalpaşa populasyonunundan rastgele seçilmiş bireylerin olgun nutletleri oluşturmaktadır. Tohum canlılığının belirlenmesinde Tetrazolyum Klorid TTC testi kullanılmıştır. Ön işlem uygulanan 45 gün ve uygulanmayan kontrol tohumlar cam petrilerde, 250 ppm, 500 ppm, 1000 ppm, 2000 ppm’deki farklı konsantrasyonlarda 10 ml Gibberellik Asit GA3 çözeltisi ve saf su kontrol ; 25°C 12 saat aydınlık A /15°C 12 saat karanlık K değişken sıcaklık-ışık ve sürekli karanlık koşullarda çimlenme tetstlerine tabi tutulmuşlardır. Tohum canlılık testleri sonucunda tohumlarda %85 oranında canlılık belirlenmiştir. 12A/12K ışık fotoperyodunda ön işlem uygulanmayan tohumlarda en yüksek çimlenme %40 ile 250 ppm de olduğu saptanırken, aynı ışık periyodunda en düşük çimlenme %6,6 ile kontrol tohumlarda olduğu saptanmıştır. Bu çalışma sonucunda, S. smyrnaea’nın ex-situ yetiştirilmesi için en yüksek çimlenme yüzdesinin, stratifikasyon 45 gün 5ºC uygulanmış ve 25/15°C değişken sıcaklıkta, sürekli karanlık koşullarda 250 ppm GA3uygulanmış tohumlarla elde edilebileceği saptanmıştır

Keywords

• Nif Dağı,Salvia smyrnaea,Tohum Çimlenmesi,Endemik

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