The genetic diversity of Iranian pistachio Pistacia vera L. cultivars revealed by ISSR markers

Abstract

Iran has a rich pistachio germplasm, thereby, the diversity and number of Iranian pistachio cultivars is unique in the world. In this study, genetic relationships among thirty one cultivars was assessed by using six inter simple sequence repeat ISSR primers. During the ISSR screening in this study, good amplification products were obtained from primers based on GA, CA and GAA repeats. Since, primers based on CT, GT and CAA repeats produced few large separate bands which were eliminated for the final analysis. The total of 28 bands of which 13 46/42% were polymorphic were amplified by the three primers, an average of 9.3 bands per primer. The total number of amplified fragments was between 7 to 12 and the number of polymorphic fragments ranged from three to five. The range of genetic similarity was from 0/84 to 1 and the constructed unweighted pair group method with arithmetic averages UPGMA dendrogram classified the tested genotypes into 11 main clusters. The present study showed that there is low genetic diversity among tested cultivars and the ISSR-PCR analysis produces sufficient polymorphisms for large-scale DNA fingerprinting. This study reports the first application of the ISSR technique in characterization of Iranian pistachio cultivars

Keywords

• Pistachio,ISSR,Genetic relationships,Clustering

References

1. Ahmad, R., Fergusen, L. and Southwick, S.M (2005). Molecular marker analysis of pistachio rootstocks by simple sequence sepeats and sequence-selated Amplified solymorphisms. Journal of Horticultural Science and Biotechnology, 80, 382-386.
2. Ahmad, R. Fergusen, L. and Southwick, S.M. (2003). Identification of pistachio (Pistacia vera L.) Nuts with microsatellite markers. Journal of American Society Horticulture Science, 121, 898-903.
3. Ahmadi Afzadi, M., Seyed Tabatabaei, B. E., Mohammadi, S. A. and Tajabadipur. A. (2007). Comparison of genetic diversity in species and cultivars of pistachio (Pistacia vera L.) based on amplified fragment length polymorphism marker. Iranian Journal of biotechnology, 5,147-152.
4. Basha. A I., Padulosi S., Chabane K., Hadji-Hasan A., Dulllo E., Pagnota M A., Porceddu E. (2007). Genetic diversity of Syrian pistachio (Pistacia vera L.) varieties evaluated by AFLP markers. Geneicst Resourse Crop Evolution, 54, 1807-1816.
5. Browiez, K. (1988). Chorology of trees and shrubs in south-west Asia and adjacent regions. Polish Scientific Publication, Warsaw, Poland.
6. Doyle, J. J. and Doyle, J. L. (1987). A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19, 11-15.
7. Esmail-Pour, A. (2001). Distribution, use and conservationof pistachio in Iran. In: Padulosi S, Hadj-Hassan A (Eds) Project on underutilized mediterranean species. Pistacia: towards a comprehensive documentation of distribution and use of its genetic diversity in central &west Asia, North Africa and mediterranean Europe. IPGRI, Rome, Italy.
8. Fang, D. Q. and Roose, M. L. (1997). Identification of closely relative Citrus cultivars with inters simple sequence repeat markers. Theoretical Applied Genetics, 95, 408-417.

9. Faostat. (2004). FAOSTAT database. FAO statistics database on The World Wide Web. http://apps.fao.org (accessed December 2004).
10. Goldhirish, G. A., Kostiukovosky, V. and Rowland, L. J. (2004). Genetic relationship among mediterrainian Pistacia species evaluated by RAPD and AFLP markers. Plant Systematics and Evolution, 246, 9-18.
11. Gupta, M., Chyi, Y .S .I., Romero-Severson, J. and Owen, J. L. (1994). Amplification of DNA markers from evolutionary diverse genomes using single primers of simple sequence repeats Theoretical. Applied. Genetics, 89, 998–1006.
12. Hansen, M., Hallena, C. and Sall, T. (1998) Error rates and polymorphism frequencies for three RAPD protocols. Plant Molecular Biology Reports, 16,139-146.
13. Hormaza J. I., Dollo, L. and Polito.V.S. (1994). Determination of relatedness and geographical movements of pistacia vera (Pistachio; Anacardiaceae) germplasm by RAPD analysis. Economic Botany, 48,349-358.
14. Hormaza, J. I., Pinney, K. and Pplito, V. S. (1998). Genetic Diversity of Pistachio(Pistacia vera, Anacardiaceae) germplasm based on randomly amplified polymorphic DNA (RAPD) markers. Economoics Botanny, 52, 78-87.
15. Ila Hb, Kafkas, S, and Topaktas, M. (2003). Chromosome numbers of Four (Anacardiaceae) species. Journal of Horticultural Science Biotechnology, 78, 35–38.
16. Jones, CJ, Edwards, KJ, Castrglione, S., Winfield, MO., Sale, F., Van de Wiel, C., Bredemeijer, G., Buiatti, M., Maestri, E., Malcevshi, A., Marmiroli, N., Aert, R., Volckaert, G., Rueda, J., Linacero, R., Vazquez, A. and Karp, A. (1997). Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Molecular Breeding, 3,381-390.
17. Kafkas, S., Ozkan, H. B., E., Acar, I., Atli, H.S. and Koyoncu, S. (2006). Detecting DNA polymorphism and genetic diversity in a wide germplasm: comparison of AFLP, ISSR, RAPD markers. American Society for Horticultural Science, 131,522-529.
18. Kafkas, S. and Perl-Treves. R. (2002). Interspecific relattionships in pistacia based on RAPD fingerprinting. Horticultural Science, 371, 168-171.
19. Karp, A., Kresovich, S., Bhat, K.V., Ayada, W.G. and Hodgkin, T. (1997). Molecular tools in plant genetic resources conservation: a guide to the technologies. IPGRI technical bulletin no 2. International Plant Genetic Resources Institute, Rome, Italy.
20. Katsiotis, A., Hagidimitricu, M., Drosso, A., Pontilis, C. and Loukas M. (2003). Genetic Relationships among Species and Cultivars of Pistacia using RAPD and AFLP Markers. Euphytica, 132, 279-288.
21. Mehlenbacher, S.A. (2003). Progress and prospects in nut breeding. Acta Horticulture, 622, 57–79.
22. Meyer, W., Michell, T.G., Freedman, E. Z. and Vilgalys, R. (1993). Hybridization probes for conventional DNA fingerprinting used as single primers in polymerase chain reaction to distinguish strain of Cryptococcus neoformans. Journal of Clinnical Biology, 31, 2274-2280.
23. Mirzaei, S., Bahar, M. and Sharifnabi, B. (2005). A phylogenetic study of Iranian wild pistachio species and some cultivars using RAPD marker. Acta Horticulture, 726, 39-43.
24. Nagaoka T, and Ogihara, Y. (1997). Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RAPD and RFLP markers. Theoretical. Applied. Genetics. 94, 597-602.
25. Pejic, I. P., Ajmone-Marsan, M., Morgante, V., Kozumplick, P., Castiglioni, G., Taramino. and MOTTO, M. ( 1998). Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs and AFLPs. Theoretical and Applied Genetics, 97, 1248-1255.
26. Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, j., Tingey, s. and Rafalski, A. (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2, 225-238.
27. Rohlf, F. J. (1998). Ntsys-pc: Numerical taxonomy and multivariate analysis system. Version 2.0. Department of ecology and evolution. State university of New York.
28. Sneath, P.H., Sokal, R. R. (1973). Numerical taxonomy: the principles and practice of numerical classification. W.h. freeman and company, San Francisco.
29. Staub, J. E., Serqun, F. C. and Gupta, M. (1996). Genetic marker, map costruction, and their application in plant breeding. HorticulturalScience, 31,729-740.
30. Tsumura, Y., Ohba, K., and Strauss, S. H. (1996). Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica). Theoretical Applied Genetics, 92, 40-45.
31. Virk, P.S., Zhu, J., Newbury, H.J., Bryan, G.J., Jackson, M.T. and Ford-Liodl B.V (2000). Effectiveness of different classes of molecular markers for classifying and revealing variations in rice (Oryza sativa) germplasm. Euphytica, 112, 275-284.
32. Whitehouse, W.E. (1957). The pistachio nut. A new crop for the Western United States. Economical. Botany, 11,281- 321.
33. Williams, J.G.K., Kubelik, A.R., Levak, K.J., Rafalski, J.A. and. Tingey, S.V.1990. DNA polymorphism amplification by arbitary primers is useful as genetics markers. Nucleic Acids Research, 18, 6531-6535.
34. Ziekiewicz, E., Rafalski, A. and Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchore PCR amplification. Genomics, 20, 176-183.
35. Zohari, M. (1952). Amorphological study of the genus Pistacia. Palestine Journal of Botany, 5, 187-196.