Pimpla turionellae L. (Hymenoptera: Ichneumonidae) Zehirinin Memeli Hücrelerindeki Potansiyel Antienflamatuvar Etkilerinin Değerlendirilmesi

Abstract

Parazitik arılar sahip oldukları kompleks bir zehir karışımı ile birlikte yumurtalarını diğer böceklerin içine veya üzerine enjekte ederler. Parazitoidlerin zehirleri, genç parazitoidin büyüme gelişimini sağlamak amacıyla konak fizyolojisini değiştirecek veya bağışıklık sistemini baskılayacak şekilde iş görmektedir. Böceklerde başlıca doğal bağışıklık yolakları olan Toll/ Imd yolağının, memelilerdeki NF-kappa B (NF-κB, Nüklear Faktör kappa B) yolağına homolog olması nedeniyle, zehire bağlı olarak konak böceğin bağışıklık sisteminde oluşan tepkilerin memeli hücre sisteminde de olabileceği çalışmamızın hipotezi olarak belirlenmiştir. Bu nedenle, P. turionellae zehrinin murin fibrosarkoma L929sA hücreleri üzerindeki etkilerini belirlemek için NF-κB 'ye bağımlı bir lusiferaz aktivite testi kullanıldı. MTT testinden elde edilen sonuçlar, P. turionellae'den elde edilen zehirinin uygulanan inkübasyon sürelerinde ve konsantrasyonlarda L929sA hücre hatları üzerinde sitotoksik etkiye sahip olmadığını göstermiştir. Ayrıca, bu çalışma, endoparazitoit P. turionellae zehirinin, toksik olmayan konsantrasyonlarında memeli hücrelerindeki NF-κB sinyalini inhibe etme potansiyeline sahip olduğunu göstermiştir. Sonuç olarak, ekto- veya endoparazitoit arılardan elde edilen zehir bileşenleri, memeli hücrelerinin artan bağışıklık tepkileri üzerinde antienflamatuvar potansiyele sahiptir.

Keywords

• endoparazitoit zehir
• fibrosarkoma hücre
• NF-κB sinyali
• enflamasyon
• Pimpla turionellae

Project Number

115Z142

Evaluation of the Potential Anti-inflammatory Effects of Endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) venom on Mammalian Cell Lines

Abstract

Parasitic wasps inject their eggs, together with a complex venom mixture, in or on other insects. Parasitoid venoms use various mechanisms to manipulate the physiology and suppress the immune system of their hosts, thus enabling the growth and development of their offspring. Since the major mechanisms of innate immunity in insects are homologous to the Nuclear Factor kappa B (NF-κB) pathway in mammalian immunity, this study hypothesized that venom related immune suppression observed in host insects could also be observed in mammalian cells. Therefore, an NF-κB-dependent luciferase assay was used to determine the effects of P. turionellae venom on murine fibrosarcoma L929sA cells. Results from an MTT assay showed that venom from P. turionellae has no cytotoxic effects on L929sA cell lines when taking into account a defined range of exposure time and concentrations. Also, the present study indicated that endoparasitoid P. turionellae venom has potential to inhibit NF-κB signaling in cells of mammals at nontoxic concentrations. In conclusion, venom components from ecto- or endoparasitoid wasps have anti-inflammatory potential on increased immune responses of mammalian cells.

Keywords

• endoparasitoid venom
• fibrosarcoma cell
• NF-κB signal
• Pimpla turionellae
• inflammation

Supporting Institution

TUBITAK-FWO Joint Research Cooperation Project

Project Number

115Z142

Thanks

This research was supported by the TUBITAK-FWO Joint Research Cooperation Project (Grant number: 115Z142). This research was also made possible by the funding of the Belgian Agency for Innovation by Science and Technology (IWT), project 141370.

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