Year 2020, Volume 13 , Issue 2, Pages 160 - 168 2020-08-15

The antiproliferative potential of isolated emodin and aloe-emodin from Rheum ribes on different cancer cell lines
The antiproliferative potential of isolated emodin and aloe-emodin from Rheum ribes on different cancer cell lines

Mehmet Kadir ERDOĞAN [1] , Can Ali AGCA [2] , İ̇brahim Halil GEÇİBESLER [3]


Herbal compounds are important resources that used in the treatment of many diseases, and are inspiring in drug design and development. Rheum ribes, a perennial native plant, has potentially diverse medicinal effects, including antioxidant, antibacterial and anti-tumor activity. The key molecules associated with anticancer activity of R. ribes in tumor formation still remains unclear. The present study was aimed to elucidate the antiproliferative effects of two purified compounds, emodin and aloe-emodin, from R. ribes. In our study, chromatographic separation was used to purification of compounds. Experimental 1D and 2D 1H and 13C-NMR spectra helped us to confirm the chemical structures of emodin and aloe-emodin. The viability of human glioblastoma cell line (U373), human breast carcinoma cells (MCF-7), and human colorectal cancer cell line (HT-29) were detected by WST-1 proliferation assay as dose- and time-dependent. The isolated emodin and aloe-emodin from R. ribes, reduced viability of human glioblastoma, breast and colorectal cancer cell lines in dose- and time-dependent manner significantly. These antiproliferative effects may contribute as a possible strategy to the development of new therapeutic approaches in the treatment of cancer.
Herbal compounds are important resources that used in the treatment of many diseases, and are inspiring in drug design and development. Rheum ribes, a perennial native plant, has potentially diverse medicinal effects, including antioxidant, antibacterial and anti-tumor activity. The key molecules associated with anticancer activity of R. ribes in tumor formation still remains unclear. The present study was aimed to elucidate the antiproliferative effects of two purified compounds, emodin and aloe-emodin, from R. ribes. In our study, chromatographic separation was used to purification of compounds. Experimental 1D and 2D 1H and 13C-NMR spectra helped us to confirm the chemical structures of emodin and aloe-emodin. The viability of human glioblastoma cell line (U373), human breast carcinoma cells (MCF-7), and human colorectal cancer cell line (HT-29) were detected by WST-1 proliferation assay as dose- and time-dependent. The isolated emodin and aloe-emodin from R. ribes, reduced viability of human glioblastoma, breast and colorectal cancer cell lines in dose- and time-dependent manner significantly. These antiproliferative effects may contribute as a possible strategy to the development of new therapeutic approaches in the treatment of cancer.
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Primary Language en
Subjects Biochemistry and Molecular Biology
Journal Section Research Articles
Authors

Orcid: 0000-0002-1579-5737
Author: Mehmet Kadir ERDOĞAN (Primary Author)
Institution: Bingol University, Faculty of Arts and Science, Department of Biology, 12000, Bingöl, Turkey
Country: Turkey


Orcid: 0000-0002-0244-3767
Author: Can Ali AGCA
Institution: BİNGÖL ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, MOLEKÜLER BİYOLOJİ VE GENETİK BÖLÜMÜ, GENETİK ANABİLİM DALI
Country: Turkey


Orcid: 0000-0002-4473-2671
Author: İ̇brahim Halil GEÇİBESLER
Institution: BİNGÖL ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ FAKÜLTESİ, İŞ SAĞLIĞI VE GÜVENLİĞİ BÖLÜMÜ
Country: Turkey


Supporting Institution This study was supported by The Scientific Research Projects Coordination Unit of Bingol University (Project no: BAP-FEF.2017.00.010).
Project Number BAP-FEF.2017.00.010
Dates

Application Date : April 15, 2020
Acceptance Date : June 30, 2020
Publication Date : August 15, 2020

APA Erdoğan, M , Agca, C , Geçi̇besler, İ . (2020). The antiproliferative potential of isolated emodin and aloe-emodin from Rheum ribes on different cancer cell lines . Biyolojik Çeşitlilik ve Koruma , 13 (2) , 160-168 . Retrieved from https://dergipark.org.tr/en/pub/biodicon/issue/54651/753046