Anatomical and micromorphological investigations on some Centaurea Asteraceae, Cardueae taxa from NE Anatolia

Year 2018, Volume: 11 Issue: 2, 29 - 41, 15.08.2018

Melahat Ozcan

Abstract

This study evaluates the phylogenetic relationships of some Centaurea s.l. taxa native to Northeast Anatolia, Turkey. Stem, leaf and cypsela anatomical structures and cypsela micromorphologies were characterized and compared in detail by using variance analysis and cluster analysis UPGMA . Macro and micromorphological characteristics of cypsela were investigated using a stereomicroscope and scanning electron microscope. Significant differences were observed in stem features, leaf midrib and lamina thickness, number of stomata, and epidermal cell wall patterns in the abaxial and adaxial surfaces among the taxa. Cypsela shapes were determined as ovoid or widely oblong. Cypselas can be distinguished into four types of surface ornamentations. The structure of the pericarp was described, as well as the testa, and the distribution of the sclerenchymatic zone of the ribs and secretory ducts resin in the mesocarp were evaluated

Keywords

anatomy, Centaurea, cypsela, micromorphology, SEM

Kuzeydoğu Anadolu Bölgesi’ndeki bazı Centaurea Asteraceae, Cardueae taksonları üzerinde anatomik ve mikromorfolojik araştırmalar

Abstract

Bu çalışma Kuzeydoğu Anadolu, Turkiye için doğal olan bazı Centaurea taksonlarının akrabalık ilişkilerini ele almaktadır. Gövde, yaprak ve sipsela anatomik yapıları ve mikromorfolojik özellikleri belirtilmekte ve varyas ile kümeleme analizi UPGMA uygulayarak detaylı olarak karşılaştırılmaktadır. Sipsela makro ve mikromorfolojik özellikleri stereomikroskop ve taramalı elektron mikroskobu kullanarak incelendi. Taksonlar arasında gövde özellikleri, yaprak orta damar ve lamina kalınlığı, stoma sayısı ve üst ile alt epidermis yüzey şekillerinde önemli farklılıklar gözlemlendi. Sipselalar şekillerine göre ovoid ve genişçe oblong olarak tanımlandı. Yüzey süslemelerine göre dört tip ayırt edildi. Testa yapısı ile perikarp yapısı tanımlandı ve damarlardaki sklerenkimatik kısımların ve mezokarpta salgı kanalarının dağılımı değerlendirildi

Keywords

Anatomi, Centaurea, sipsela, mikromorfoloji, SEM

References

• Algan, G. (1981). Bitkisel Dokular için Mikroteknik. Fırat Universitesi Fen Fakültesi Yayınları, Bot. No.1. İstanbul. Matbaa Teknisyenleri Basımevi (in Turkish).
• Andrés-Sánchez, S., Galbany-Casals, M., Bergmeier, E., Rico, E., Martínez-Ortega, M. M. (2015). Systematic significance and evolutionary dynamics of the achene twin hairs in Filago (Asteraceae, Gnaphalieae) and related genera: further evidence of morphological homoplasy. Plant Systematics and Evolution, 301, 1653-1668.
• Aydin, Ö., Coşkunçelebi, K., Gültepe, M., Güzel, M.E. (2013). A contribution to taxonomy of Centaurea including Psephellus (Asteraceae) based on anatomical and molecular data. Turkish Journal of Botany, 37, 419-427.
• Abid, R., Ali, N. (2010). Cypsela morphology and its taxonomic significance for the tribe Senecioneae (Asteraceae) from Pakistan. Pakistan Journal of Botany, 42 (SI), 117-133.
• Abid, R.D., Qaiser, M. (2009). Taxonomic significance of the cypsela morphology in the tribe Anthemideae (Asteraceae) from Pakistan and Kashmir. Pakistan Journal of Botany, 41(2), 555-579.
• Bayrakdar, F., Aytac, Z., Suludere, Z., Candan, S. (2010). Seed morphology of Ebenus L. species endemic to Turkey. Turkish Journal of Botany, 34, 283-289.
• Bona, M. (2014). Achene characteristics of Turkish Centaurea (Asteraceae) and their systematic application. Bangladesh Journal of Botany, 43, 163-16.
• Bona, M. (2015). Systematic implications of achene characteristics in genera Centaurea L., Cyanus Mill., Psephellus Cass. and Rhaponticoides Vaill. (Asteraceae). Bangladesh Journal of Botany, 22(2), 125-136.
• Brummitt, R.K. (2004). Report of the committee for spermatophyta: 54. Taxon, 53, 813-825.
• Candan, F., Uysal, T., Tugay, O., Bozkurt, M., Ertuğrul, K., Demirelma, H. (2016). The examinations of achene ultrastructural features of section Acrolophus (Centaurea, Asteraceae) via scanning electron microscopy. Turkish Journal of Botany, 40, 147-163.
• Celik, S., Uysal, T., Menemen, Y. (2008). Morphology, anatomy, ecology and palynology of two Centaurea species from Turkey. Bangladesh Journal of Botany, 37, 67-74.
• Davis, P.H., Tan, K., Mill, R.R. (eds.) (1988). Flora of Turkey and the East Aegean Islands, vol. 10 (suppl. 1): Edinburgh University Press, Edinburgh, pp. 164-165.
• Dittrich, M. (1985). Morphologische und anatomische Untersuchungen an Blüten und Früchten der Gattung Carlina (Compositae). I Teil. Botanische Jahrbücher für Systematik, 107, 591-609.
• Duran, A., Duman, H. (2002). Two new species of Centaurea (Asteraceae) from Turkey. Annales Botanici Fennici, 39, 43-48.
• Eminagaoglu, O., Ozcan, M. (2014). Morphology and leaf anatomy of some Euonymus taxa from Turkey. Biodicon 7(3), 167-176.
• Greuter W (2003a). The Euro + Med treatment of Cardueae (Compositae) generic concepts and required new names. Willdenowia, 33, 49-61.
• Greuter, W. (2003b). The Euro + Med treatment Senecioneae and the minor Compositae tribes-generic concepts and required new names, with an addentum to Cardueae. Willdenowia, 33, 245-250.
• Güner, A. Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (eds). (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler), İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği yayınları, 141-146.
• Häffner, E. (2000). On the phylogeny of the subtribe Carduinae (tribe Cardueae, Compositae). Englera, 21, 1-208.
• Inceer, H., Hayirlioglu-Ayaz, S., Ozcan, M. (2007). Chromosome numbers of the twenty-two Turkish plant species. Caryologia, 60, 349-357.
• Inceer, H., Ozcan, M. (2011). Leaf anatomy as an additional taxonomy tool for 18 taxa of Matricaria L. and Tripleurospermum Sch. Bip. (Anthemideae-Asteraceae) in Turkey. Plant Systematics and Evolution, 296, 205-215.
• Jana, B.K., Mukherjee, S.K. (2012). Comparative morphological and anatomical studies of cypselas of some members of the tribe Millerieae (Asteraceae). Internatioanal Journal of Pharmaceutical Research and Bio-Science, 1(5), 317-330.
• Kadereit, J.W., Jeffrey, C. (eds.) (2007). Flowering Plants. Eudicots: Asterales. In the Families and Genera of Vascular Plants (K Kubitzki, ed.), vol. 8, Springer, Berlin.
• Kaya, A., Dirmenci, T. (2008). Nutlet surface micromorphology of the genus Nepeta L. Lamiaceae) in Turkey. Turkish Journal of Botany, 32, 103-112.
• Kaya, Z., Orcan, N., Binzet,R. (2010). Morphological, anatomical and palynological study of Centaurea calcitrapa L. subsp. cilicica (Boiss. & Bal.) Wagenitz and Centaurea solstitialis L. subsp. carneola (Boiss.) Wagenitz endemic for Turkey. Pakistan Journal of Botany, 42, 59-69.
• Kulkarni, S.V. (2013). SEM studies of Achenes in some taxa of Asteraceae. International Journal for Environmental Rehabilitation and Conservation, 4(2), 87-97.
• Kose, Y.B., Yücel, E. (2007). An investigation on comparison of ecological and biological properties of two endemic Centaurea species from Turkey (Centaurea lycia and Centaurea luschaniana). International Conference on Environment: Survival and Sustainability Proceedings, 2, 659-670. Turkish Republic of Northern Cyprus.
• Lavialle, P. (1912). Recherches sur le developpement de l’ovaire en fruit chez les Composées. Annales des Sciences Naturalles- Botanique Ser., 9. 15, 39-149.
• LoPresti, R.M., Oppolzer, S., Oberprieler, C. 2010. A molecular phylogeny and revised classification of the Mediterranean genus Anthemis s.l. (Compositae, Anthemideae) based on three molecular markers and micromorphological characters. Taxon, 59(5), 1441-1456.
• Martin, E., Dinc, M.,Duran, A. (2009). Karyomorphological study of eight Centaurea L. taxa (Asteraceae) from Turkey. Turkish Journal of Botany, 33, 97-104.
• Metcalfe, C.R., Chalk, L. (1979). Anatomy of Dicotyledones I. Oxford. University Press, pp. 783-803.
• Meidner, H., Mansfield, T.A. (1968). Physiology of Stomata. London: McGraw-Hill.
• Mukherjee, S. K. (2001). Cypselar features nineteen taxa of the tribe Senecioneae (Asteraceae) and their taxonomic significance. Journal of Economic & Taxonomic Botany (Recent Researches in Plant Anatomy and Morphology), Additional Series No. 19.
• Ozcan, M. (2017). Cypsela micromorphology and anatomy in Cirsium sect. Epitrachys (Asteraceae, Carduoideae) and its taxonomic implications. Nordic Journal of Botany, 35, 653-668.
• Ozcan, M., Demiralay, M., Kahriman, A. (2015). Leaf anatomical notes on Cirsium Miller (Asteraceae, Carduoideae) from Turkey. Plant Systematics and Evolution, 301, 1995-2012.
• Ozcan, M., Unver, M.C., Eminagaoglu, O. (2014). Comparative anatomical and ecological investigations on some Centaurea (Asteraceae) taxa from Turkey and their taxonomic significance. Pakistan Journal of Botany, 46 (4), 1287-1301.
• Özcan, M. (2013). Türkiye’de Yetişen Psephellus pulcherrimus (syn: Centaurea pulcherrima var. freynii) (Cardueae, Asteraceae)’un morfolojik ve anatomik Özellikleri. ACU Journal of Forest Faculty, 14(1), 104-112.
• Pandey, A.K., Singh, R.P. (1982). Development and structure of seeds and fruits in the Compositae, tribe Senecioneae. Botanische Jahrbücher für Systematik, 103, 413-422.
• Pinar, N.M., Duran, A., Çeter, T., Tug, G.N. (2009). Pollen and seed morphology of the genus Hesperis L. (Brassicaceae) in Turkey. Turkish Journal of Botany, 33, 83-96.
• Romaschenko, K., Ertugrul, K., Susanna,A., Garcia-Jacas, N., Uysal,T., Arslan, E. (2004). New chromosome counts in the Centaurea jacea group (Asteraceae, Cardueae) and some related taxa. Botanical Journal of the Linnean Society, 145, 345- 352.
• Singh, R. P., Pandey, A. K. (1984). Development and structure of seeds and fruits in Compositae-Cynareae. Phytomorphology, 34(1- 4), 1-10.
• Shabestari, E.S.B., Attar, F., Riahi, H., Sheidai, M. (2013). Seed morphology of the Centaurea species (Asteraceae) in Iran. Phytologia Balcanica, 19 (2), 209-214.
• SheikhAkbari, R., Azizian, D. (2006). Seed morphology and seed coat sculpturing of Epilobium L. species (Onagraceae Juss.) from Iran. Turkish Journal of Botany, 30, 435-440.
• Stearn, W.T. (1985). Botanical Latin. 3th edition. David & Charles Publishers, USA, ISBN 0715385488.
• Stace, C.A. (1984). The Taxonomic Importance of the Leaf Surface. In: Heywood, V. H., Moore D. M., eds. Current concepts in plant taxonomy, 25. Systematic association special Academic Press, London.
• Susanna, A., Garcia-Jacas, N. (2007). Tribe Cardueae. In: Kadereit, J. W., Jeffrey, C. (eds.), The Families and Genera of Vascular Plants, 8, 123-146, Springer Verlag, Berlin, Heidelberg & New York.
• Şirin, E., Ertuğrul, K., Uysal, T. (2017). Achene micromorphology of the genus Cyanus Mill. (Compositae) in Turkey and its taxonomic importance. Phytotaxa, 313 (1), 77-90.
• Tarıkahya-Hacıoğlu, B., Arslan, Y., Subaşı, İ., Katar, D., Bülbül, A.S., Çeter, T. (2012). Achene morphology of Turkish Carthamus species. Australian Journal of Crop Science, 6(8), 1260-1264.
• Tekeli, Y., Sezgin, M., Aktumsek,A., Gule, G.O., Sanda, M.A. (2010). Fatty acid composition of six Centaurea species growing in Konya, Turkey. Natural Product Research, 24, 1883-1889.
• Tetley, U. (1925). The secretory system of the roots of the Compositae. New Phytologist, 24, 138-162.
• Uysal,I., Celik, S., Menemen, Y. (2005). Morphology, anatomy, ecology, pollen and achene features of Centaurea polyclada DC. (Sect. Acrolophus) in Turkey. International Journal of Biological Sciences, 5, 176-180.
• Wagenitz, G. (1975). Centaurea L. In: Davis, P. H. (ed.) Flora of Turkey and the East Aegean Islands, 5, Edinburgh University Press, Edinburgh, pp.465-585.
• Wagenitz, G., Hellwig, F.H. (1996). Evolution of characters and phylogeny of the Centaureinae. In: Hind, D. J. N., Beentje, H. G. (eds.), Compositae: Systematics. Proceedings of the international Compositae conference Royal Botanic Gardens, Kew, pp. 491-510.
• Wagenitz, G., Hellwig,F.H. (2000). The genus Psephellus Cass. (Compositae, Cardueae) revisited with a broadened concept. Willdenowia, 30, 29-44.
• Williams, B.C. (1954). Observations on intercellular canals in root tips with special reference to the Compositae. American Journal of Botany, 41, 104-106.
• Zarembo, E.V., Boyko, E.V. (2008). Carpology of some East Asian Cardueae (Asteraceae). Anales del Jardin Botánico de Madrid,(1), 129-134.
• Zhu, S.X., Qin, H.N., Shih, C. (2006). Achene wall anatomy and surface sculpturing of Lactuca L. And related genera (Compositae: Lactuceae) with notes on their systematic significance. Journal of Integrative Plant Biology, 48(4), 390- 399.