Changes in pigment content of green algae Desmodesmus sp. and Chodatodesmus mucranulatus exposed to alumina oxide Al2O3 nanoparticles

Abstract

Nanoparticles NPs have emerged as a new class of environmental pollutants with the emergence of nanotechnology. Al2O3 NPs released from different industries, personal care products and wide range of applications necessarily end up in aquatic environments. Algal growth inhibition tests are an significant indicator model of monitoring programs designed to predict the effect of NPs on aquatic environments. This study investigated the effects of varyingduration and concentration exposure on the chlorophyll Chl contents of Al2O3 NPs to two species of freshwater green algae Desmodesmus sp., and Chodatodesmus mucranulatus recommended for use in standard algal growth inhibition tests. To induce Al2O3 NPs effect, we exposed algae to 3- 96 mg/L for 96 hours. In the test groups treated with Al2O3 NPs in both algae cells, chlorophyll content decreased in 48 hours exposure compared to the control groups and a clear increase in exposure time to 72 hours was observed. As a result, it was noted that the chlorophyll content of this study varied at the varying duration and concentrations. Variation in chlorophyll Chla and Chlb concentrations for Desmodesmus sp. and Chodatodesmus mucranulatus.was recorded at the significance level of p

Keywords

• alumina,chlorophyll,nanotoxicology,nanoparticles,microalgae

Alümina oksit Al2O3 nanopartiküllerine maruz kalan yeşil alglerin Desmodesmus sp. ve Chodatodesmus mucranulatus pigment içeriğindeki değişiklikler

Abstract

Nanopartiküller NP’ler , nanoteknoloji ile birlikte çevre kirleticilerinin yeni bir sınıfı olarak ortaya çıkmıştır. Al2O3 NP’leri kişisel bakım ürünleri, farklı endüstriler ve geniş uygulama yelpazesinden salınır ve mutlaka sucul çevrelere ulaşır. Algal büyüme inhibisyon testleri, bu sucul çevrelerde NP’lerin etkisini öngörmek için tasarlanmış izleme programlarının önemli bir göstergesidir. Bu çalışmada, Al2O3 NP’lerinin değişen süresi ve konsantrasyonlarda, iki tatlısu yeşil alg Desmodesmus sp. ve Chodatodesmus mucranulatus türlerinde, standart alg büyüme inhibisyon testi ile klorofil klf muhteviyatını üzerine etkisi araştırılmıştır. Al2O3 NP etkisini değerlendirmek için algler 72 saat boyunca 3-96 mg/L konsantrasyonlarında Al2O3 NP’lerine maruz bırakıldı. Al2O3 NP’leri uygulanan test gruplarında, kontrol grupları ile karşılaştırıldığında klorofil muhtevasında net bir azalma gözlemlendi. Her iki alg hücresinde Al2O3 NP’leri ile muamele edilen test gruplarında klorofil muhteviyatı kontrol gruplarına kıyasla 48 saat sonra azaldı, maruz kalma süresi 72 saate uzadığında ise belirgin bir klorofil muhteviyatında artış gözlendi. Sonuç olarak, bu çalışmanın klorofil muhteviyatı Al2O3NP’lerinin değişen süre ve konsantrasyonlarda değiştiği kaydedilmiştir. Desmodesmus sp. ve Chodatodesmus mucranulatus için klorofil klfa ve klfb konsantrasyonlarında değişimi p

Keywords

• alüminyum oksit,klorofil,nanotoksikoloji,nanopartiküller,mikroalgler

References

1. Arul Prakash, F., Dushendra Babu, G.J., Lavanya, M., Shenbaga Vidhya, K., Devasena, T. (2011). Toxicity studies of aluminium oxide nanoparticles in cell lines. Int J Nanotechnol Appl, 5(2), 99-107.
2. Burklew, C.E., Ashlock, J., Winfrey, W.B., Zhang, B. (2012). Effects of aluminum oxide nanoparticles on the growth, development, and microRNA expression of tobacco (Nicotiana tabacum). PloS one, 7(5), e34783.
3. Coşan, E.D., Tezcan, N., Coşan, D.T. (2015). The effects of chemical and biological plant fertilizer types for used to ıncrease agricultural production on Chlorophyll A, Chlorophyll B, Vitamin C, the length of plants, mitosis and chromosomes of Onion (Allium cepa). Biological Diversity and Conservation, 8(2) (2015) 16-22
4. Çiçek, L.N., Ertan, O.Ö., Erdoğan, Ö., Didinen H., Boyacı, Ö.Y., Kara, D., Zeybek, M., Diken, G. (2017). Distribution of phytoplankton and its relationship with physicochemical parameters in Lake Eğirdir (Isparta/Turkey). Biological Diversity and Conservation, 10(3), 150-162
5. Çolak, A.D., Nas, B. (2016). NiFe2O4 Nanokompozitinin Olası Toksik Etkisine Karşı Olueropein’in Koruyucu Rolü. Erzincan University Journal of Science and Technology, 9 (Special Issue I), 172-183.
6. Dağlıoğlu Y, Özturk Y.B. (2016b). Desmodesmus multivariabilis’in bor partikullerine maruz kalmada biyolojik birikiminin değerlendirilmesi. Biological Diversity and Conservation. 9(3), 204–209.
7. Dağlıoğlu, Y., Çelebi, S.M., Önalan, Ş., (2016a). Determination of Acute Toxic Effects of Poly (Vinylferrocenium) Supported Palladium Nanoparticle (Pd/PVF+ ) on Artemia salina . Pakistan Journal of Zoology vol. 48(1), 187-193.
8. Dağlıoğlu, Y., Öztürk, Y.B. (2018). Effect of concentration and exposure time of ZnO-TiO2 nanocomposite on photosynthetic pigment contents, ROS production ability, and bioaccumulation of freshwater algae. Caryologia, 71(1), 13-23.

9. Dağlıoğlu, Y., Türkiş S., (2017). TiO2 nanopartikül Uygulamasının Su Mercimeğinin (Lemna minor L.) Fotosentetik Pigment İçeriği Üzerine Etkisi. Acta Biologica Turcica, 30(4), 108-115.
10. García-Saucedo, C., Field, J.A., Otero-Gonzalez, L., Sierra-Álvarez, R. (2011). Low toxicity of HfO2, SiO2, Al2O3 and CeO2 nanoparticles to the yeast, Saccharomyces cerevisiae. Journal of hazardous materials, 192(3), 1572-1579.
11. Gosteva, I., Morgalev, Y., Morgaleva, T., Morgalev, S. (2015). Effect of Al2O3 and TiO2 nanoparticles on aquatic organisms. In IOP Conference Series: Materials Science and Engineering, Vol. 98, No. 1, p. 012007. IOP Publishing.
12. Huang, Y.W., Wu, C.H., Aronstam, R.S. (2010). Toxicity of transition metal oxide nanoparticles: recent insights from in vitro studies. Materials, 3(10), 4842-4859.
13. Jeng, H. A., Swanson, J. (2006). Toxicity of metal oxide nanoparticles in mammalian cells. Journal of Environmental Science and Health Part A, 41(12), 2699-2711.
14. Knox, J.P. (1995). The extracellular matrix in higher plants. 4. Developmentally regulated proteoglycans and glycoproteins of the plant cell surface. The FASEB Journal, 9(11), 1004-1012.
15. Kulacki, K. J., Cardinale, B. J. (2012). Effects of nano-titanium dioxide on freshwater algal population dynamics. PLoS One, 7(10), 1-7.
16. Lichtenthaler, H. K., Wellburn, A. R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents, 603rd Meeting, Liverpool, 591-592.
17. Nowack, B., Bucheli, T.D. (2007). Occurrence, behavior and effects of nanoparticles in the environment. Environmental pollution, 150(1), 5-22.
18. Oberdorster, G., Oberdorster, E., Oberdorster, J., (2005). Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environmental Health Perspectives, 113, 823–839.
19. Özkan, Y., İrende, İ., Akdeniz, G., Kabakçı, D., Sökmen, M., (2015). Evaluation of the Comparative Acute Toxic Effects of TiO2, Ag- TiO2 and ZnO-TiOComposite Nanoparticles on Apis mellifera (Honey Bee) . Jornal of International. Environmental Application&Science, 10(1), 26-36.
20. Pakrashi, S., Dalai, S., Sabat, D., Singh, S., Chandrasekaran, N., Mukherjee, A. (2011). Cytotoxicity of Al2O3 nanoparticles at low exposure levels to a freshwater bacterial isolate. Chemical Research in Toxicology, 24(11), 1899-1904.
21. Qukarroum, A., Bras, S., Perreault, F., Popovic, R. (2012). Inhibitory effects of silver nanoparticles in two green algae, Chlorella vulgaris and Dunaliella tertiolecta. Ecotoxicology and Environmental Safety, 78, 80-85.
22. Rippka, R. (1988). [1] Isolation and purification of cyanobacteria. In Methods in enzymology, Vol. 167, 3-27. Academic Press.
23. Sadiq, I.M., Chowdhury, B., Chandrasekaran, N., Mukherjee, A. (2009). Antimicrobial sensitivity of Escherichia coli to alumina nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine, 5(3), 282-286.
24. Sadiq, I.M., Pakrashi, S., Chandrasekaran, N., Mukherjee, A. (2011). Studies on toxicity of aluminium oxide (Al2O3) nanoparticles to microalgae species: Scenedesmus sp and Chlorella sp. Journal of Nanoparticle Research, 13, 3287-3299
25. Shelknanloymılan, L., Atıcı, T., Obal, O. (2012). Removal of nitrogen and phosphate by using Choleralla vulgaris on synthetic and organic materials waste water. Biological Diversity and Conservation. 5/2 (2012) 89-94.
26. Shrivastava, R., Raza, S., Yadav, A., Kushwaha, P., Flora, S.J. (2014). Effects of sub-acute exposure to TiO2, ZnO and Al2O3 nanoparticles on oxidative stress and histological changes in mouse liver and brain. Drug and chemical toxicology, 37(3), 336- 347.
27. Stanier, R. Y., Kunisawa, R., Mandel, M., Cohen-Bazire, G. (1971). Purification and properties of unicellular blue-green algae (Order Chroococcales). Bact. l~ev. 85, 171-205.
28. Test, O.A.G.I. (1984). OECD guideline for testing of chemicals 201. Paris, France: Organisation of Economic Cooperation and Development.
29. Wang, H., Wick, R. L., Xing, B. (2009). Toxicity of nanoparticulate and bulk ZnO, AlO3 and TiO2 to the nematode Caenorhabditis elegans. Environmental Pollution, 157(4), 1171-1177.
30. Zhang, X. Q., Yin, L. H., Meng, T.A.N.G., Pu, Y.P. (2011). ZnO, TiO2, SiO2, and Al2O3 nanoparticles-induced toxic effects on human fetal lung fibroblasts. Biomedical and Environmental Sciences, 24(6), 661-669.