Alkaliphilic bacterial diversity of Lake Van/Turkey

Year 2017, Volume: 10 Issue: 1, 92 - 103, 15.04.2017

Nilgün Poyraz Mehmet Burçin Mutlu

Abstract

Lake Van, situated in Turkey's eastern Anatolian region, is a saline soda lake and it covers the lowest part of a vast basin. The importance of Lake Van comes from being one of the largest soda lakes and third largest endorheic lake on Earth with a volume of 607 km3. Although several biological studies have been done for Lake Van, research on microbial diversity of the lake is still missing. This paper contributes to scientific knowledge by research on alkaliphilic bacterial diversity of Lake Van. In this regard, culture-dependent and culture-independent methods were applied to the samples, which were taken from different locations close to the shores of surrounding cities. Isolation was performed on 10 different media. Bacterial diversity was analyzed by 16S rRNA gene PCR amplification, sequencing and blast analysis against NCBI database. Results showed that members of Proteobacteria especially Gammaproteobacteria and Actinobacteria phylums were represented dominantly. Culture-independent DGGE Denaturing Gradient Gel Electrophoresis and FISH Fluorescence In-Situ Hybridization methods were also used to detect the microbial populations in Lake Van. The results showed that the community was dominated by Bacteria

Keywords

Lake Van, alkaliphilic bacteria, ARDRA, DGGE, FISH

Van Gölü’nün alkalifilik bakteriyel çeşitliliği

Abstract

Van Gölü Türkiye Doğu Anadolu Bölgesi’nde yer alan, büyük bir havzanın alçak kısmını kaplayan tuzlu bir soda gölüdür. Van Gölü’nün önemi Dünya’nın en büyük soda göllerinden bir olmasından ve 607 km3’lük hacmi ile Dünya’nın en büyük 3. kapalı havza gölü olmasından kaynaklanmaktadır. Van Gölü ile ilgili bazı biyolojik çalışmalar yapılmasına rağmen mikrobiyal çeşitliliği üzerine araştırmalar yeterli değildir. Bu yüzden bu çalışma Van Gölü'ndeki alkalifilik bakteri çeşitliliği üzerine yapılan araştırmalara bilimsel katkılar sağlamaktadır. Bu amaçla, gölün çevresindeki şehirlerin kıyılara yakın, farklı noktalardan alınan su örneklerine kültür bağımlı ve kültürden bağımsız yöntemler uygulanmıştır. Kültür çalışmaları için 10 farklı besi yeri kullanılmıştır. Bakteriyel çeşitlilik, 16 S rRNA geninin PCR ile çoğaltılması, dizileme ve NCBI veri tabanı kullanılarak incelenmiştir. Sonuçlar, Proteobacteria özellikle Gammaproteobacteria ve Actinobacteria filumlarının baskın bir şekilde bulunduğunu göstermiştir. Mikrobiyal populasyonları belirlemek için kültür bağımsız yöntemler olan DGGE Denatüre Edici Gradiyent Jel Elektroforezi ve FISH Fluoresan In Situ Hibridizasyon kullanılmıştır. Sonuçlar kommunitede bakterilerin yoğun olduğunu göstermiştir

Keywords

Van Gölü, alkalifilik bakteri, ARDRA, DGGE, FISH

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