Contents of phenolic and flavonoid compounds in Isatis demiriziana Mısırdalı: an endemic to the Southeast Anatolia, Turkey

Year 2017, Volume: 10 Issue: 1, 115 - 123, 15.04.2017

Özgür Karakaş Mustafa Abdullah Yılmaz Hilal Surmuş Asan Ahmet Onay Nazan Çalar

Abstract

Isatis demiriziana Mısırdalı plant contains a number of compounds which has anticarcinogenic, antioxidant and other preventive effects. In this study, the flavonoid and phenolic contents in the plant samples harvested in the vegetative leaf and root and full flowering season flower, leaf and root were determined by LC-MS/MS. Among the 27 compounds studied, malic acid was found to be the most abundant compound in the methanolic extracts of samples and the amount of malic acid of vegetative root extracts were the highest 30124,37 µg g-1 dry-extract . Moreover, it was also determined considerable amount of salicylic acids and p-coumaric in the root extracts. This study is the first detailed study on the phenolic and flavonoid compounds of I. demiriziana. Based on the findings of this study, in further researches might be refered as an additional source in production of phenolic and flavonoid compounds

Keywords

Isatis, LC–MS/MS, phenolics, flavonoids, endemic

Türkiye’de Güneydoğu Anadolu Bölgesi’ne endemik Isatis demiriziana Mısırdalı’ndaki fenolik ve flavonoid bileşiklerinin içerikleri

Abstract

Isatis demiriziana Mısırdalı bitkisi antikanserojen, antioksidan ve başka koruyucu özelliklere sahip olan çok sayıda bileşik içermektedir. Bu çalışmada, I. demiriziana’nın vejetatif yaprak ve kök ve tam çiçeklenme çiçek, yaprak ve kök dönemlerinde hasat edilen bitki örneklerinin flavonoid ve fenolik içerikleri LC-MS/MS ile tespit edildi. Çalışılan 27 bileşik arasında, örneklerin metanolik ekstraktlarında malik asit miktarı en fazla düzeyde bulundu ve vejetatif köklerdeki malik asit miktarı 30124,37 µg g-1 kuru ekstrakt en yüksek orana sahipti. Ayrıca kök ekstraktlarında büyük miktarda salisilik asit ve p-kumarik asit tespit edildi. Bu çalışma, I. demiriziana’nın fenolik ve flavonoid içerikleri üzerine yapılmış ilk detaylı çalışmadır. Bu çalışmanın sonuçlarına dayanılarak, ileriki çalışmalarda fenolik ve flavonoid bileşiklerin üretiminde bir ek kaynak olarak başvurulabilir

Keywords

Isatis, LC–MS/MS, fenolikler, flavonoidler, endemik

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