Rhizopogon roseolus Corda Th. Fr.’ un kültürel özellikleri

Year 2017, Volume: 10 Issue: 3, 79 - 84, 15.04.2017

Perihan Güler

Abstract

Bu çalışmada Rhizopogon roseolus Corda Th. Fr.’un spor ve miselyumlarının anatomik ve morfolojik özellikleri çalışıldı. Mantar örnekleri Kırıkkale-Türkiye’den toplandı ve laboratuvara getirildi. Alınan doku örnekleri patates dekstroz agar PDA besiyerinde doku kültürü yöntemi ile gelişti. 25˚C’de inkübe edildi. Miseller inokulasyondan 2 gün sonra besiyeri üzerinde karışık olarak gelişim gösterdi. Özellikle koloninin en dış bölgesindeki misellerde yoğunluğun arttığı ve pigmentasyon oluşumu gözlendi. Misellerde önce hafif sarımsı renkte gelişme olduğu daha sonra koloninin en dışında pembemsi pigmentasyon geliştiği görüldü. Kolonizasyon oluşumu 20 günde tamamlandı R.roseolus’un spor ve miselleri hem ışık hem de scanning elektron mikroskop SEM ile incelendi. R.roseolus’un sporları düz ve boyutları 3.5-4x6.5-7 µm. olup genellikle oval şekillidir

Keywords

Rhizopogon roseolus , Türkiye mikobiyotası , Kırıkkale makromantarları , miselyum gelişimi

Cultural characteristics of Rhizopogon roseolus Corda Th. Fr

Abstract

In this study, the morphological and anatomical features of Rhizopogon roseolus Corda Th. Fr. spores and mycelium were examined. The mushroom samples were collected from Kırıkkale-Turkey and brought to the laboratory. Taken tissue fragments were grown on potato dextrose agar PDA medium by tissue culture method. They were incubated in 25˚C. After 2 days from inoculation mycelium showed mixed growth on the agar medium. Particularly, in the outermost regions of the colony, density increased and pigmentation was observed. It was first observed to develop a slightly yellowish color and then pinkish pigmentation developed at the outermost part of the colony. Colonization occurred in 20 days. The spores and mycelium of R.roseolus were examined by both light microscopy and scanning electron microscopy SEM . The spores of R. roseolus are smooth and have dimensions of .5-4x6.5-7 µm. usually oval shape

Keywords

Rhizopogon roseolus , Turkey mycobiota , Kırıkkale macrofungus , mycelium development

References

• Aktaş, S., Öztürk, C., Kaşık, G., Sabahlar, Ş., Doğan, H.H., (2003). Macrofungi Flora of Bozkır District (Konya) Turk J Bot 27, 37-43
• Alkan, S., Kaşık, G., Öztürk, C., Aktaş, S., (2016). Edible Macrofungi of Çorum Province. Turkish Journal of Agriculture. Vol 4, No 3, 131-136
• Anonim, (2017). Index fungorum, http://www.indexfungorum.org/names/names.asp (Erişim tarihi, 06.04.2017)
• Arora, D., 1986. Mushroom Demystified. Berkeley, California. ISBN 0-89815-567-8.
• Ashkannehhad, S., Horton, T.R.,(2006). Ectomycorrhizal ecology under primary succession on coastal sand dunes: interactions involving Pinus contorta, suilloid fungi and deer.New Phytologist. 169 (2): 345–354.
• Binder, M., Hibbett, D.S., (2006). Molecular systematics and biological diversification of Boletales. Mycologia. 98 (6): 971–981.
• Colgan III, W., Claridge, A.W., (2002). Mycorrhizal effectiveness of Rhizopogon spores recovered from faecal pellets of small forest-dwelling mammals. Mycological Research. 106 (3): 314–320.
• Doğan, H.H., Küçük, M.A., Akata, I., (2010). A Study on Macrofungal diversity of Bozyazı Province (Mersin), Turkey. GU J Sci 23(4):393-400.
• Gezer, K., Kaygusuz, O., Soylu, U., Ermiş, A., (2011). Macrofungi of Pamukkale University Kınıklı Campus (Denizli/Turkey). Biological Diversity and Conservation, 4/3, 36-43.
• Hayat, M.A., (1972). Basic Electron Microscopy Techniques. Van Nostrand Reinhold Company. Canada.
• Islam, F., Ohga, S., (2012). The response of fruit body formation on in situ condition Tricholoma matsutake by applying electric pulse stimulator. ISRN Agronomy, vol. 2012, Article ID 462724, 6 pages, 2012.
• Islam, F., Ohga, S., (2013). Effects of Media Formulation on the Growth and Morphology of Ectomycorrhizae and Their Association with Host Plant. SRN Agronomy, Volume 2013 (2013), Article ID 317903, 12pages.
• Izzo, A.D., Meyer, M., Trappe, J.M., North, M., Bruns, T.D.,(2005). Hypogeous ectomycorrhizal fungal species on roots and in small mammal diet in a mixed conifer forest. Forest Science. 51 (3): 243–254.
• Karkouri, K.E., Martin, F., Mousain, D., (2002). Dominance of the mycorrhizal fungus Rhizopogon rubescens in a plantation of Pinus pinea seedlings inoculated with Suillus collinitus. Annals of Forest Science. 59 (2): 197–204.
• Kaşık, G., Öztürk, C., Doğan, H.H., (2000). Ermenek (Karaman) Yöresinin Makrofungusları. S.Ü.Fen-Edebiyat Fakültesi Fen Dergisi Araştırma Makalesi Cilt 1, 61-65, Konya.
• Kaşık, G., Öztürk, C., (2000). Hadim ve Taşkent (Konya) Yöresinin Makrofungusları. S.Ü.Fen-Edebiyat Fakültesi Fen Dergisi Sayı 17, 1-6, Konya.
• Kaşık, G., Öztürk, C., Aktaş, S., Alkan, S., Eroğlu, G., (2013). Kefe Yaylası Yenen Mantarları. Mantar Dergisi, 4 (2) 19-27.
• Kotter, M., Farentinos, R.C., (1984). Formations of Ponderosa pine ectomycorrhizae after inoculation with feces of tassel-earred squirrels. Mycologia. 76 (2): 758–760.
• Köstekçi, H., Yamaç, M., Solak, M.H., (2005). Macrofungi of Türkmenbaba Mountain (Eskişehir). Turk J Bot 29, 409- 416.
• Maser, C., Maser, Z., (1988). Interactions among squirrels, mycorrhizal fungi, and coniferous forests in Oregon. Western North American Naturalist. 48 (3): 358–369.
• Mleczko, P., (2004). Mycorrhizal and saprobic macrofungi of two zinc wastes in Southern Poland. Acta Biologica Cracoviensia Series Botanica 46: 25–38.
• Molina, R; Trappe, J.M.; Grubisha, L.C.; Spatafora, J.W.,(1999). Rhizopogon. In Cairney, JWG; Chambers, SM. Ectomycorrhizal Fungi Key Genera in Profile. Heidelberg: Springer Berlin. pp. 12161. doi:10.1007/978-3-662- 06827-4-5. ISBN 978-3-642-08490-4.
• Nakano, S., Gao,Q., Aimi,T.,Shimomura, N., (2016). Rhizopogon roseolus basidiospore germination decreases as the fruiting bodies mature and the spore wall becomes more complex. Botany, 2016, 94(4): 311-320, 10.1139/cjb-2015- 0198
• Öztürk, C., Kaşık, G., Doğan, H.H., Aktaş, S., (2003). Macrofungi of Alanya District. Turk J Bot 27,303-312
• Shimomura, N., Aimi, T., Matsumoto, T., Maekawa, N., Otani, H., (2008). Ultrastructure of developing basidiospores in Rhizopogon roseolus (=R.rubescens). Mycoscience, 49: 35. doi: 10. 1007 / s10267-007-0388-y.
• Smith, A.H., Zeller, S.M., (1966). A preliminary account of the North American species of Rhizopogon. Mem New York Bot Gard 14:1–178.
• Trappe, M., Evans, I., Trappe, J., (2007). Field guide to North American Truffles. Berkeley, CA: Ten Speed Press. pp. 136 p. ISBN 1580088627.
• Twieg, B.D., Durall, D.M., Simard, S.W.,(2007). Ectomycorrhizal fungal succession in mixed temperate forests. New Phytologist. 176 (2): 437–447.
• Yağız, D., Afyon, A., Konuk, M., (2005). The Macrofungi of Karabük Province. Turk J Bot 29, 345-353.
• Yun, W., Hall, I.R., (2004). Edible ectomycorrhizal mushrooms: challenges and achievements. Canadian Journal of Botany. 82 (8): 1063–1073.
• Yun, W., Hall, I.R., Evans, L.A., (1997). Ectomycorrhizal fungi with edible fruiting bodies 1. Tricholoma matsutake and related fungi. Economic Botany, vol. 51, no. 3, pp. 311–327, 1997.