Phylogenetic relationships and taxonomical positions of two new records Melanoleuca species from Hakkari province, Turkey

Year 2017, Volume: 10 Issue: 3, 85 - 93, 15.04.2017

İsmail Acar Ayten Dizkırıcı Tekpınar L Ayşenur Kalmer Yusuf Uzun

Abstract

The aim of the current study is to contribute to the knowledge of the genus Melanoleuca in Turkey. Delimitation of species taxonomic position within the genus is invaluable to protect the genus. Therefore, both morphological and molecular characters were used for identification of Melanoleucaspecimens. Samples collected from Hakkâri province of Turkey were studied morphologically based on pileus, lamellae, stipe, spore, basidia and cystidia structures. Then, DNA sequence data of the Internal Transcribed Spacer ITS , the Large Subunit LSU of the nuclear ribosomal RNA rRNA gene complex and Translation Elongation Factor 1-α TEF regions were analysed to figure out phylogenetic positions within the genus. At the end of the study, two new records species of Melanoleuca M. communis and M. dryophila were reported from Hakkâri province. When ITS and LSU regions were analyzed, M. communis placed within subgenus Melanoleuca / section Melanoleuca and M. dryophila placed within subgenus Urticocytis / section Grammopodiae. Considering the lack of information of this genus in Turkey and the fact that no detailed study has been developed before, this work is valuable to the systematics of Melanoleuca and to the taxonomic knowledge of agarics in Turkey

Keywords

ITS, LSU, Morphology, Melanoleuca, Phylogeny

Türkiye, Hakkari ilinde bulunan üç yeni kayıt Melanoleuca türünün filogenetik ilişkileri ve taksonomik pozisyonları

Abstract

Çalışmamızın amacı Türkiye'de bulunan Melanoleuca cinsine ait literatür bilgisine katkıda bulunmaktır. Cins içindeki türlerin taksonomik konumlarının belirlenmesi, cinsi korumak için çok önemlidir. Bu nedenle toplanan Melanoleuca örneklerinin tanımlanması için hem morfolojik hemde moleküler karakterler kullanılmıştır. Hakkâri ilinden toplanan örnekler, şapka, lamel, spor, bazidya ve sistidya gibi yapılar kullanılarak morfolojik tanımlamaları yapılmıştır. Daha sonra, Transkribe Edilen Aralayıcı Bölgeler ITS , Büyük Altünite LSU ve Translasyon Uzama Faktörü 1-α TEF bölgelerinin DNA dizi verileri türlerin filogenetik konumlarını cins içinde anlamak için analiz edilmiştir. Çalışmanın sonunda Hakkâri ilinden iki yeni Melanoleuca türü M. communis ve M.dryophila rapor edilmiştir. ITS ve LSU bölgeleri analiz edildiğinde, M. communis, alt cins Melanoleuca / seksiyon Melanoleuca'da ve M. dryophila alt cins Urticocytis / seksiyon Grammopodiae’da yer almıştır. Türkiye'de bu cins hakkında yetersiz bilgi olması ve daha önce hiçbir ayrıntılı araştırma yapılmadığı göz önüne alındığında, bu çalışma Melanoleuca'nın sistematiği ve Türkiye'de agariklerin taksonomik bilgisi açısından değerlidir

Keywords

ITS, LSU, Morfoloji, Melanoleuca, Filogeni

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