In vitro seed germination study in endemic plant Onosma discedens

Year 2016, Volume: 9 Issue: 1, 92 - 96, 15.04.2016

Muhip Hilooğlu Ersin Yücel Ali Kandemir Emel Sözen

Abstract

Onosma discedens is endemic species having considerable narrow distribution in Erzincan Turkey Region. In this study, we investigated the germination behavior of Onosma discedens with different concentrations of NaCI, HCl, KNO3, GA3 100 and 200 μM or hot-cold stratification/mechanical scarification. Seeds under the light and dark treatments were exposed to a photoperiod of 8 h light/16 h dark hours with a 23/18 °C thermoperiod. Germination rates increased with KNO3-100 µM % 12 , GA3-100 µM % 25 , GA3-200 µM % 35 , HCI-100 µM % 9 treatments when compared to the control. The highest speed of germination index was obtained at concentrations of 100-200 µM KNO3. Overall, our results will provide valuable data for ex situ conservation of endemic Onosma discedens

Keywords

Onosma discedens, endemic, germination, ex situ

Endemik Onosma discedens tohumlarında İn vitro çimlendirme çalışmaları

Abstract

Onosma discedens Kemaliye Emzikotu , Erzincan yöresinde Türkiye oldukça dar yayılış alanına sahip nadir endemik bitki türlerindendir. Bu çalışmada Onosma discedens türünde farklı NaCl, HCl, KNO3, GA3 konsantrasyonları ve sıcak/soğuk/mekanik stratifikasyon uygulamalarının çimlenme başarısına etkilerinin incelenmesi amaçlanmıştır. Tohumlar 100 ve 200 μM konsantrasyonlarda NaCl, HCl, KNO3, GA3 solusyonları kullanılarak ya da sıcak/soğuk/mekanik stratifikasyon uygulamalarının ardından 23/18°C’de 8 saat aydınlık/16 saat karanlık ortamda çimlendirilmiştir. Kontrol grubundan % 7 , daha yüksek çimlenme gerçekleşen uygulamalar; KNO3-100 µM % 12 , GA3-100 µM % 25 , GA3-200 µM % 35 , HCI-100 µM % 9 . Çimlendirme hızı katsayısının ise en yüksek potasyum nitratın uygulamalarında 9,5 olduğu bulunmuştur. Bu çalışma sonucunda, elde edilen veriler türün ex-situ korunması amacıyla kullanılabilinir

Keywords

Onosma discedens, endemik, çimlenme, ex situ

References

• Bonner, F.T. 1974. Seed testing in Seed of woody plants in the United States. Agriculture handbook No. 450. For Service USDA Washington.
• Bu, H., Du, G., Chen, X., Xu, X., Liu, K., Wen S. 2008. Community wide germination strategies in an alpine meadow on the eastern Qinghai-Tibet plateau: phylogenetic and life-history correlates. Plant Ecology. 195/87–98
• Cerabolini, B., Andreis, R. De, Ceriani, R. M., Pierce, S., & Raimondi, B. 2004. Seed germination and conservation of endangered species from the Italian Alps : Physoplexis comosa and Primula glaucescens. Biological Conservation. 117: 351-356.
• Corral-Aguirre, J., & Sanchez-Velasquez, L. R. 2006. Seed ecology and germination treatments in Magnolia dealbata: An endangered species. Flora - Morphology, Distribution. Functional Ecology of Plants. 201/3: 227–232.
• Cousins, S. R., Witkowski, E. T. F., & Mycock, D. J. 2014. South African Journal of Botany Seed storage and germination in Kumara plicatilis , a tree aloe endemic to mountain fynbos in the Boland, South-Western Cape , South Africa. South African Journal of Botany. 94: 190–194.
• Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. ve Adıgüzel N. 2000. Red Data Book of Turkish Plants (Pteridophyta ve Spermatophyta). Türkiye Tabiatı Koruma Derneği ve Van 100. Yıl Üniv. Ankara.
• El-Shazly A, Abdel-Ghani A & Wink M. 2003. Pyrrolizidine alkaloids from Onosma arenaria (Boraginaceae). Biochem Syst and Ecol. 31: 477-485.
• Iglesias, R.G., Babiano M.J. 1997. Endogenous abscisic acid during the germination of chickpea seed. Physiologia Plantarum. 100: 500-504.
• Jolliff, G.D., Seddigh, M., Franz R.E. 1994. Seed germination and dormancy of new meadowfoam (Limnanthes spp.) genotypes. Industrial Crops and Products. 2/3: 179-187.
• Kandemir, A. 2009. The rediscovery of some taxa thought to have been extinct in Turkey. Turkish J. Bot., 33: 113-122.
• Kandemir, A., & Türkmen, Z. 2010. A new species of Onosma ( Boraginaceae ) from eastern Turkey. 34: 277-282.
• Nadjafi, F., Bannayan, M., Tabrizi, L., Rastgoo M. 2006. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environments. 64/3: 542–547.
• Navarro, L. & J. Guitian. 2003. Seed germination and seedling survival of two threatened endemic species of the northwest Iberian Peninsula. Biological Conservation. 109: 313-320
• Puppala, N., James L., Fowler L. J. 2002. Lesquerella seed pretreatment to improve germination. Industrial Crops and Products. 64- 69.
• Teimouri, M. S., & Mahallati, M. N. 2013. Seed germination and breaking of seed dormancy techniques for endemic Hymenocrater platystegius. International Journal of Agriculture and Crop Sciences. 6/12: 885–889
• Yücel, E. 2000. Effects of different salt (NaCl), potasyum nitrate (KNO3) and acid (H2SO4) concentrations on the germination of some Salvia species seeds. Seed Science & Technology. 28: 853-860.