ISSR variations of four populations of Glycyrrhiza glabra Fabaceae

Year 2016, Volume: 9 Issue: 2, 24 - 29, 15.08.2016

Houshang Nosratı Mohammadali Hosseınpour-feızı Marjan Bagherı Ahmad Razban- Haghıghı

Abstract

Information on patterns and structure of populations’ genetics in plants are useful in understanding breeding systems, population dynamics and designing conservation programs. Glycyrrhiza glabra L. liquorice, Fabaceae is a perennial plant and has high commercial uses for medicinal, flavouring and sweeting purposes. However, there is little information on the genetic structure of liquorice. In the current study the levels of genetic variations were investigated in four eco-geographically different populations of liquorice using Inter Simple Sequence Repeats ISSRs by randomly sampling 10 individual plants from each of population in East-Azerbaijan Province, Iran. The population genetic variation were measured based on Nei's and Shannon's information indices using Popgen, and the genetic similarity among the populations was studied by the clustering analysis of Unweighted Pair-Group Method with Arithmetical Averages UPGMA . Total genetic variation was partitioned into within and among the populations using analysis of molecular variance AMOVA by Arlequin. A total of 139 polymorphic reproducible ISSRs loci were obtained. The percentage of polymorphic ISSRs loci ranged from 35.97% to 61.15%. Consequently, the range of within-population Nei's diversity varied from 0.172 to 0.301. Partitioning of total ISSRs variation by AMOVA showed that total genetic variation was equally partitioned to within-population and among-populations, indicating that the species has intermediate breeding systems by carrying out equally self- and outcrossing. Nei’s based UPGMA dendrogram showed no ecological correlation among the populations. Moreover, the genetic similarity among populations was not correlated with the geographical distances Spearman rho correlation, N=6, P > 0.787 , while as expected, the populations size affected significantly on population genetic variation Spearman, N=4, P < 0.05 . This study emphasizes the application of conservation program in the study sites in order to prevent the potential risk of the species extinction

Keywords

conservation programs , genetic diversity , Glycyrrhiza glabra , ISSRs , population size

References

• Allnutt, T.R., Courtis, J. R., Gardner, M., Newton A. C. 2001. Genetic variation in wild Chilean and cultivated British populations of Podocarpus salignus D. Don (Podocarpaceae). Edinburgh Journal of Botany, 58: 459-473.
• Andrieu, E., Dornier, A., Rouifed, S., Schatz, B., Cheptou, P. O. 2009. The town Crepis and the country Crepis: How does fragmentation affect a plant–pollinator interaction? Acta Oecologia, 35: 1-7.
• Ashraf, J., Malik, W., Iqbal, M.Z., Ali-Khan, A., Qayyum, A., Noor, E., Abid, M.A., Naseer-Cheema, H.M., Ahmad, M.Q. 2016. Comparative analysis of genetic diversity among Bt cotton genotypes using EST-SSR, ISSR and Morphological Markers. Journal of Agricultural Science and Technology, 18: 517-531.
• Bornet, B., Branchard, M. 2001. Nonanchored Inter Simple Sequence Repeat (ISSR) markers: Reproducible and specific tools for genome fingerprinting. Plant Molecular Biological Reports, 19: 209-215.
• Bozkurt, M., Ertuğrul, K., Uysal, T. 2013. The determination of genetic relationships among some Vicia L. (Vetch) taxa by using ISSR markers. Biological Diversity and Conservation, 6/3: 135-139.
• Brunet, J., Echert, C.G. 1998. Effects of floral morphology and display on outcrossing in Blue Columbine Aquilegia caerulea (Ranunculaceae). Functional Ecology, 12: 596–606.
• Davis, E. A., Morris, D. J. 1991. Medicinal uses of liquorice through the millennia the good and plenty of it. Molecular Cell Endocrinology, 78: 1-6.
• Chen, L., Chen, F., He, S., Ma, L. 2014. High genetic diversity and small genetic variation among populations of Magnolia wufengensis (Magnoliaceae), revealed by ISSR and SRAP markers. Electronical Journal of Biotechnology, 17/6: 268–274.
• Excoffier, L., Smouse, P.E., Quattro, J. M. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes application to human mitochondrial DNA restriction data. Genetics 131: 479-491.
• Fahima, T., Sun, G. L., Beharav, A., Krugman, T., Beiles A., Nevo E. 1999. RAPD polymorphism of wild emmer wheat populations Triticum dicoccoides in Israel. Theoretical and Applied Genetics, 98: 434-447.
• Fahrig, L. 2003. Effects of habitat fragmentation on biodiversity. Annual Review of Ecology, Evolution, and Systematics, 34: 487- 515.
• Feder, M. E., Mitchell-Olds, T. 2003. Evolutionary and ecological functional genomics. Nature Reviews Genetics, 4: 651-657.
• Frankham, R. 1996. Relationship of genetic variation to population size in wildlife. Conservation Biology, 10: 1500-1508.
• Frankhamn, R. 2003. Genetics and conservation biology. Comptes rendus Biologies, 326: S22-S29.
• Jennersten, O. 1988. Pollination in Dianthus deltoids (Caryophyllaceae). Effects of habitat fragmentation on visitation and seed set. Conservation Biology, 2: 359-366.
• Hamrick, J.L., Godt M.J.W. 1989. Allozyme diversity in plant species In. Brown A. H. D., Clegg M. T., Kahler A. L., Weir B. S. (Eds.) Plant population genetics breeding and genetic resources. Sinauer Associates Sunderland MA, USA.
• Hamrick, J.L., Godt, M.J.W. 1996. Effects of life history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society of London, Sec. B, 351: 1292-1298.
• Lande, R. 1988. Genetics and demography in biological conservation. Science, 241: 1455-1460.
• Lande, R., Barrowclough, G.F. 1987. Effective population size genetic variation and their use in population management. In: Soule M. (ed) Viable populations for conservation. Cambridge University Press, England.
• Li, H., Chen, G. 2008. Genetic relationship among species in the genus Sonneratia in China as revealed by inter-simple sequence repeat (ISSR) markers. Biochemical Systematics and Ecology, 36: 392-398.
• Lienert, J. 2004. Habitat fragmentation effects on fitness of plant populations - a review. Journal for Nature Conservation, 12: 53-72.
• Moulin, M.M., Rodrigues, R., Gonçalves, L.S.A., Sudré, C.P., Pereira M.G. 2012. A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.). Acta Scientifica, 34/2: 139-147.
• Mukherjee, A., Sikdar, B., Ghosh, B., Banerjee, A., Ghosh, E., Bhattacharya, M., Roy S.C. 2013. RAPD and ISSR analysis of some economically important species, varieties, and cultivars of the genus Allium (Alliaceae). Turkish Journal of Botany, 37: 605- 618.
• Nei, M., Maruyama, T., Chakraborty, R. 1975. The bottleneck effect and genetic variability in populations. Evolution, 29: 1-10.
• Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of the Academy of Natural Sciences of Philadelphia, USA, 70: 3321-3323.
• Nevo, E., Noy-Meir, I., Beiles A. 1991. Natural selection of allozyme polymorphism.micro-geographical spatial and temporal ecological differentiation in wild emmer wheat. Israel Journal of Botany, 40: 419-450.
• Nevo, E., Baum, B., Beiles A., Johnson D.A. 1998. Ecological correlates of RAPD DNA diversity of wild barley Hordeum spontaneum in the Fertile Crescent. Genetic Resources and Crop Evolution, 45: 151-159.
• Nianxi, Z., Yubao, G., Jinlong, W., Anzhi, R., Hua X. 2006. RAPD diversity of Stipa grandis populations and its relationship with some ecological factors. Acta Ecological Sinaica, 26: 1312-1319.
• Nomura, T., Fukai, T., Akiyama, T. 2002. Chemistry of phenolic compounds of licorice (Glycyrrhiza species) and their estrogenic and cytotoxic activities. Pure and Applied Chemistry, 74: 1199-1206.
• Nybom, H., Bartish, I.V. 2000. Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspectives in Plant Ecology, Evolution and Systematics, 3: 293-114.
• Rakoczy-Trojanowska, M., Bolibok, H. 2004. Characteristics and a comparison of three classes of microsatellite-based markers and their application in plants. Cellular and Molecular Biology Letters, 9: 221-238.
• Reddy, P.M., Sarla, N., Siddiq, E.A. 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128: 9-17.
• Reed, D.H., Frankham R. 2003. Correlation between fitness and genetic diversity. Conservation Biology, 17: 230-237.
• Richards, A.J. 1986. Plant breeding systems. George Allen and Unwin London, England.
• Rich, S.S., Bell, A.E., Wilson, S.P. 1979. Genetic drift in small populations of Trifolium. Evolution, 33: 579-584.
• Wang, H.Z., Feng, S.G., Lu, J.J., Shi, N.N., Liu, J.J. 2009. Phylogenetic study and molecular identification of 31 Dendrobium species using inter-simple sequence repeat (ISSR) markers. Science Horticulturae, 122: 440-447.
• Wilcock, C., Neiland, M.R.M. 2002. Pollination failure in plants why it happens and when it matters. Trends in Plant Science, 7: 270-277.
• Young, A.G., Clarke, G.M. 2000. Genetics demography and viability of fragmented populations conservation biology. Cambridge University Press, Cambridge.
• Yuan, C.Y., Zhang, C., Wang, P., Hu, S., Chang, H.P., Xiao, W.J., Lu, X.T., Jiang, S.B., Yu, J.Z., Guo, X.H. 2014. Genetic diversity analysis of okra (Abelmoschus esculentus L.) by inter-simple sequence repeat (ISSR) markers. Genetic Molecular Research, 13/2: 3165 -3175.
• Zietkiewitcz, E., Rafalski, A., Labuda, D. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20: 176-183.
• Zhao, X., Ma, Y., Sun, W., Wen, X., Milne, R. 2012. High Genetic Diversity and Low Differentiation of Michelia coriacea (Magnoliaceae), a Critically Endangered Endemic in Southeast Yunnan, China. International Journal of Molecular Science, 13: 4396-4411.