Isolation and identification of a novel thermo-alkalophilic Anoxybacillus sp. strain KB4 from Kuşburnu hot spring in Turkey

Year 2016, Volume: 9 Issue: 2, 169 - 179, 15.08.2016

Fatma Matpan Bekler

Abstract

Thermophiles are able to grow at temperatures up to 110°C and at different pH thermo-acidophiles and thermo-alkalophiles and they are an interesting source of biotechnological applications. The aim of this study was to isolate thermophilic bacterium from Kuşburnu hot water spring in the town of Diyadin in Turkey. The thermophilic strain KB4 was identified by morphological, physiological, biochemical tests and 16S rRNA gene sequencing. Strain KB4 was found to be an aerobic, Gram-positive, rod shaped, motile and yellow-pigmented. Optimal growth was obtained at pH 9.0–10.0, at 55-60 oC, and at 3% w/v NaCl. Potantial of different carbon and nitrogen sources utilization were examined. The strain KB4 was able to utilize some carbon and nitrogen sources, including glucose, galactose, sucrose, maltose, arabinose, xylose, yeast extract, peptone, tryptone and casamino acids. Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate KB4 was most closely related to Anoxybacillus pushchinoensis K1 T with a 98.78% sequence similarity. In addition, this study will be a guiding study in determining the diversity of thermophilic bacteria from geothermal waters of Turkey

Keywords

Anoxybacillus, isolation, identification, thermophilic, 16S rRNA gene

Kuşburnu sıcak su kaynağından yeni termo-alkalofilik Anoxybacillus sp. KB4 şusunun izolasyonu ve tanımlanması

Abstract

Termofiller 110 oC’ye kadar sıcaklıklarda ve farklı pH’larda termo-asidofil ve termo-alkalofil büyüyebilme yeteneğine sahiptirler ve biyoteknolojik uygulamaların ilgi çeken bir kaynağıdırlar. Bu çalışmanın amacı, Türkiye’deki Diyadin ilçesi Kuşburnu sıcak su kaynağından termofilik bakteri izole etmektir. KB4 suşu, morfolojik, fizyolojik, biyokimyasal testler ve 16S rRNA gen sekanslamayla tanımlanmıştır. KB4 suşu, aerobik, Gram-pozitif, çubuk şeklinde, hareketli ve sarı pigmentli olarak bulunmuştur. Optimum üreme pH 9.0-10.0’da, 55-60 oC’de ve %3 w/v NaCl konsantrasyonunda elde edilmiştir. Farklı karbon ve azot kaynaklarının kullanımı denendi. KB4 suşu, glukoz, galaktoz, sükroz, maltoz, arabinoz, ksiloz, yeast ekstrakt, pepton, tripton ve casamino asit gibi bazı karbon ve azot kaynaklarını kullanabilmektedir. 16S rRNA gen sekansına bağlı filogenetik analizler KB4 suşunun %98.78 sekans benzerliği ile Anoxybacillus pushchinoensis K1 T ’e oldukça yakın olduğu göstermektedir. Ek olarak, bu çalışma Türkiyede’ki jeothermal sulardan termofilik bakterilerin çeşitliliğini belirlemede rehber olacaktır

Keywords

Anoxybacillus, izolasyon, tanımlama, termofilik, 16S rRNA geni

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