The first study in Turkey about the chromosomes of tongue fish, Pegusa lascaris Risso, 1810 Soleidae, Pleuronectiformes , living in the Black Sea

Year 2016, Volume: 9 Issue: 3, 1 - 7, 15.12.2016

Serkan Saygun Filiz Saygun

Abstract

In this study, It was examined the chromosome structures, numbers and other cytogenetic features of sole Pegusa lascaris Risso, 1810 being one of the flatfish species live in the Black Sea, by conventional banding methods. The Sole specimens were obtained from fishermen along the coast of West and Central Black Sea between Zonguldak and Ordu . Gill and fin epithelia tissues derived by dissecting fish samples which used colchicine 0.05% treatment for six hours were incubated in hypotonic solution 0.4% KCl and then treated with Carnoy’s fixative. As a result of karyological examination, it was determined that the chromosome number of P. lascaris showed 2n= 42 including 6 metacentric, 8 submetacentric, 12 subtelocentric and 16 acrocentric chromosomes NF= 56

Keywords

Tongue fish, Pegusa lascaris, Pleuronectiformes, flatfishes, karyotype

Karadeniz'de yaşayan dil balığı, Pegusa lascaris Risso, 1810 Soleidae, Pleuronectiformes kromozomları konusunda Türkiye'deki ilk çalışma

Abstract

Bu çalışmada; Karadeniz’de yaşayan yassı balık Pleuronectiformes türlerinden dil balığının Pegusa lascaris Risso, 1810 kromozom yapıları, sayıları ve sitogenetiksel özellikleri rutin bantlama yöntemleri kullanılarak incelenmiştir. Araştırmada dil balığı örnekleri Batı ve Orta Karadeniz sahili boyunca Zonguldak ve Ordu illeri arası bazı bölgelerden avcılıkla elde edilmiştir. Altı saat süre ile kolşisin % 0,05 muamelesi uygulanan balık örneklerinden diseksiyonla çıkarılan solungaç ve yüzgeç epitel dokuları % 0,4 KCl solüsyonunda inkübe edilmiştir ve sonrasında Carnoy fiksatifi ile muamele edilmiştir. Yapılan karyolojik incelemeler sonucunda P. lascaris balığının 42 diploit sayıda ve 6 metasentrik, 8 submetasentrik, 12 subtelosentrik ve 16 akrosentrik kromozomdan meydana gelen karyotipi NF=56 olduğu tespit edilmiştir

Keywords

Dil balığı, Pegusa lascaris, Pleuronectiformes, yassı balıklar, karyotip

References

• Akşıray, F. (1987). Türkiye Deniz Balıkları ve Tayin Anahtarı. İstanbul: İstanbul Üniversitesi Yayınları.
• Arai, R. (2011). Fish Karyotypes: A Check List. Tokyo: Springer.
• Barker, C.J. (1972). A method for the display of chromosomes of plaice, Pleuronectes platessa, and other marine fishes. Copeia, 2, 362-365.
• Berendzen, P.B., Dimmick, W.W. (2002). Phylogenetic relationships of Pleuronectiformes based on molecular evidence. Copeia, 3, 642-652. Stable URL: http://www.jstor.org/stable/1448146 (10.02.2005)
• Berg, L.S. (1947). Classification of fishes, both recent and fossil. Russia: Trav. Institute of Zoological Academy of Sciences.
• Borsa, P., Quignard, J.P. (2001). Systematics of the Atlantic-Mediterranean soles Pegusa impar, P. lascaris, Solea aegyptiaca, S. senegalensis, and S. solea and S. solea (Pleuronectiformes: Soleidae). Canadian Journal of Zoology, 79, 2297-2302. DOI: 10.1139/z01-176
• Chapleau, F. (1993). Pleuronectiform relationships: a cladistic reassessment. Bulletin of Marine Science, 52, 516-540.
• Cross, I., Merlo, A., Manchado, M., Infante, C., Cañavate, J.P., Rebordinos, L. (2006). Cytogenetic characterization of the sole Solea senegalensis (Teleostei: Pleuronectiformes: Soleidae): Ag-NOR, (GATA)n, (TTAGGG)n and ribosomal genes by one-color and two-color FISH. Genetica, 128, 253-259. DOI: 10.1007/s10709-005-5928-9
• Demirsoy, A. (2005). Yaşamın Temel Kuralları Omurgalılar/Anamniyota . Cilt- 3/Kısım 1. Ankara: Meteksan A.Ş.
• Denton, T.E. (1973). Fish Chromosome Methodology. NewYork: Charles C. Thomas Publisher.
• Duran-Gonzalez, A., García-Rucias, C.E., Laguarda-Figueras, A. (1990). The karyotype and G-bands of Haemulon aurolineatum Cuvier 1829 (Pisces: Hamulidae). Anales Del Instituto De Ciencias Del Mar Y Limnologia, 17, 2, 1- 10.
• Dutrillaux, B., Coutourier, J. (1981). La pratigue de l’analyse chromosomique techniques de laboratoire. No 12. Paris: Masson S.A. Pub.
• Emiroğlu, Ö., Uyanoğlu, M., Başkurt, S., Sülün, Ş., Köse, E., Tokatlı, C., Uysal, K., Arslan, N., Çiçek, A. (2013).
• Erythrocyte deformations in Rutilus rutilus provided from Porsuk Dam Lake. Biological Diversity and Conservation, 6(1), 13-17.
• Froese, R., Pauly, D. (2016). Fishbase. http://www.fishbase.org version (Accessed Date: 01.02/2016).
• García-Cegarra, A., Merlo, M.A., Ponce, M., Portela-Bens, S., Cross, I., Manchado, M., Rebordinos, L. (2013). A Preliminary Genetic Map in Solea senegalensis (Pleuronectiformes, Soleidae) Using BAC-FISH and NextGeneration Sequencing. Cytogenetic and Genome Research, 141, 227-240. DOI: 10.1159/000355001
• Helfman, G.S., Collette, B.B., Facey, D.E., Bowen, B.W. (2009). The diversity of fishes: Biology, Evolution, and Ecology (2nd ed.). West Sussex: John Wiley & Sons Ltd.
• Jankun, M., Ocalewicz, K., Pardo, B.G., Martinez, P., Woznicki, P., Sanchez, L. (2003). Chromosomal characteristics of rDNA in European grayling Thymallus thymallus (Salmonidae). Genetica, 119, 219-224. DOI: 10.1023/A:1026022415908
• Karahan, A. (2016). Karyotype analysis and chromosome banding of Upeneus moluccensis (Bleeker, 1855) from the north-eastern Mediterranean. Caryologia, 69, 2, 141-146. DOI: 10.1080/00087114.2016.1139415
• Kligerman, A.D., Bloom, S.E. (1977). Rapid chromosome preparations from solid tissues of fishes. Journal of the Fisheries Research Board of Canada, 34, 266-269.
• Levan, A., Fredga, K., Sandberg, A.A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52, 201-220. DOI: 10.1111/j.1601-5223.1964.tb01953.x
• Libertini, A., Mandrioli, M., Colomba, M.S., Bertotto, D., Francescon, A., Vitturi, R. (2002). A cytogenetic study of the common sole, Solea solea, from the northern Adriatic Sea. Chromosome Science, 6, 63–66.
• Molina-Luzon, M.J., Lopez, J.R., Robles, F., Navajas-Perez, R., Ruiz-Rejon, C., De La Herran, R., Navas, J.I. (2015).
• Chromosomal manipulation in Senegalese sole (Solea senegalensis Kaup, 1858): Induction of triploidy and gynogenesis. Journal of Applied Genetics, 56, 77–84. DOI: 10.1007/s13353-014-0233-x
• Nelson, J.S., Grande, T., Wilson, M.V.H. (2016). Fishes of the World. (5th ed.). Hoboken, New Jersey: John Wiley and Sons Inc.
• Pardo, B.G., Bouza, C., Castro, J., Martinez, P., Sanchez, L. (2001). Localization of ribosomal genes in Pleuronectiformes using Ag-, CMA3-banding and in situ hybridization. Heredity, 86, 531-536. DOI: 10.1046/j.1365-2540.2001.00802.x
• Patro, R., Prasad, R.P.G. (1981). Chromosomal studies in five Indian flatfishes. Copeia, 2: 498-503.
• Reeves, A. (2001). MicroMeasure: A new computer program for the collection and analysis of cytogenetic data. Genome, 44, 439-443. DOI: 10.1139/g01-037
• Romer, A.S. (1960). The Vertebrate Story (4th ed.). Chicago: University of Chicago Press.
• Rossi, A.R., Crosetti, D., Gornung, E., Sola, L. (1996). Cytogenetic analysis of global populations of Mugil cephalus (Striped mullet) by different staining techniques and fluorescent in situ hybridization. Heredity, 76, 77-82. DOI: 10.1038/hdy.1996.10
• Sofradzija, A. (1985). Citogenticka istrazivanja Jadranskilriba Uransocopus scaber L., Solea lutea (Risso), Serranus scriba (L.). Biljeske Notes, 64, 1-8.
• Thorgaard, G.H., Disney, J.E. (1990). Chromosome Preparation and Analysis. In: Methods for Fish Biology (Schreck C.B. & Moyle P.B., eds.) (pp. 171-190). Bethesda, Maryland: American Fisheries Society.
• Vasiliev, V.P. (1978). Kariotipy plati vidov ryb Tschernogo morja. Tsitologia, 20, 1092-1094.
• Vega, L., Díaz, E., Cross, I., Rebordinos, L. (2002). Caracterización citogenética e isoenzimá tica del lenguado Solea senegalensis Kaup, 1858. Boletín Instituto Español De Oceanografía, 18, 245–250.
• Vitturi, R., Catalano, E., Schillaci, A. (1993). Karyotypic characterization of 16 Microchirus ocellatus (L.) specimens (Pisces, Soleidae) using conventional and silver staining (NORs). Caryologia, 46(1), 41-45. DOI: 10.1080/00087114.1993.10797244.