Long-term germplasm conservation of two economical important Musa species via cryopreservation-dehidration technique

Year 2016, Volume: 9 Issue: 3, 178 - 182, 15.12.2016

Ergun Kaya

Abstract

An important alternative technique for plant germplasm conservation is offered by a biotechnology-based approache of cryopreservation and it refers to storage of plant material at ultra-low temperatures in liquid nitrogen. A procedure for cryopreservation of seeds was improved for two different Musa sp. in current study. Seed dehydration was performed at different exposure times, in the sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to cryopreservation when the seeds were dehydrated to a moisture content of 16.7% M. velutina H. Wendl. & Drude to 16.3% M. acuminata Colla subsp. burmanica Simmonds and the post-cryopreservation germinability also ranged respectively from 84.3% to 64.2%. Dehydration was beneficial for germination of all seeds of the tested Musa species after the liquid nitrogen exposure and the cryopreserved seedlings had well-formed shoot and roots, and their acclimatization to greenhouse conditions was easy

Keywords

Cryopreservation, Musa spp., viability and germination, seed moisture content, dehydration

Kriyoprezervasyon-dehidratasyon tekniği kullanılarak ekonomik öneme sahip iki Musa türünün germplazmasının uzun süreli saklanması

Abstract

Bitki germplazmasının korunmasında önemli, alternatif ve biyoteknoloji tabanlı bir teknik, sıvı azot içerisinde bitki materyallerinin ultra düşük sıcaklıklarda saklanması anlamına gelen kriyoprezervasyondur. Mevcut çalışmada iki farklı Musa sp.’ye ait tohumların kriyoprezervasyonu için bir yöntem geliştirilmiştir. Steril koşullarda, yatay akımlı kabin içerisinde farklı uygulama sürelerinde tohum dehidratasyonu gerçekleştirilmiştir. Test edilen kültür çeşitlerinde tohum nem içerikleri % 16.7 M. velutina H. Wendl. & Drude için ve % 16.3 M. acuminata Colla subsp. burmanica için oranlarına düşürüldüğünde, en yüksek kriyoprezervasyon toleransı görülmüştür ve ayrıca kriyoprezervasyon sonrası çimlenme de sırasıyla % 84.3 ve % 64.2 aralığında olmuştur. Dehidratasyon, sıvı azot uygulaması sonrası test edilen Musa türlerine ait tüm tohumlarının çimlenmesi üzerinde olumlu etkiye sahipti, kriyoprezervasyon sonrası elde edilen fidelerden gelişen sürgün ve kökler iyi formdaydı ve sera koşularına iklimlendirilmesi kolay oldu

Keywords

Kriyoprezervasyon, Musa spp., canlılık ve çimlenme, tohum nem içeriği, dehidratasyon

References

• Alao, J. S. (2009). Need for biodiversity conservation in Nasarawa State, Nigeria. Biological Diversity and Conservation, 2 (1), 14- 20.
• Bajaj, Y. P. S. (1995). Biotechnology in agriculture and forestry cryopreservation of plant germplasm I New York, Springer-Verlage.
• Belous, A. M., Grishchenko, V. I. (1994). Cryobiology. Naukova Dumka, Kiev.
• Benson, E. E. (1999). Plant cryopreservation, A Practical Guide. New York, Springer.
• Buitink J., Leprince, O. (2004). Glass formation in plant anhydrobiotes: survival in the dry state. Cryobiology, 48 (3), 215-228.
• Chetverikova, E. P. (2008). Dehydration in cryopreservation of moist plant tissues and seed maturation. Biophysics, 53 (4), 304-307.
• Chin, H. F. (1996). Germination and Storage of Banana Seeds. Kuala Lumpur.
• Crowe, J. H., Crowe, L. M., Carpenter, J. F., Rudolph A. S., Wistrom C. A., Spargo B. J., Anchordoguy T. J. (1988). Interactions of sugars with membranes. Biochimica et Biophysica Acta, 947 (2), 367-384.
• Gakhova, E. N., Uteshev, V. K., Shishova, N. V., Yashina, S. G. (2006). The Biophysics of the Live. Cell: Conservation of Genetic Resources. Moscow.
• González-Benito, M. E., Clavero-Ramírez, I., López-Aranda, J. M. (2004). The use of cryopreservation for germplasm conservation of vegetatively propagated crops. Spanish Journal of Agricultural Research, 2, 341-351.
• Kaya, E., Alves, A., Rodrigues, L., Jenderek, M., Hernandez-Ellis, M., Ozudogru, A., Ellis, D. (2013). Cryopreservation of Eucalyptus Genetic Resources. Cryoletters, 34 (6), 608-618.
• Kaya, E., Souza, F. V. D., Yılmaz Gökdoğan, E., Ceylan, M., Jenderek M. (2016). Cryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen. Turkish Journal of Biology, doi: 10.3906/biy-1603-92.
• Manuil’skii, V. D. (1992). Establishment of Frost and Cold Hardiness in Plants. Naukova Dumka, Kiev.
• Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
• Pixton, S. W. (1966). Moisture Content-Its Significance and Measurement in Stored Products. Journal of Stored Products Research, 3, 35-47.
• Rao, N. K. (2004). Plant genetic resources: Advancing conservation and use through biotechnology. African Journal of Biotechnology, 3(2), 136-145.
• Sharrock, S., Engels, J. (1997). Complementary Conservation. INIBAP annual report 1996, Montpellier (FRA).
• Steadman, K., Pritchard, H. W., Dey, P. M. (1996). Tissue-specific soluble sugars as indicators of seed storage category. Annals of Botany, 77(6), 667-674.
• Sun, W. O., Davidson, P., Chan, H. S. O. (1998). Protein stability in the amorphous carbohydrate matrix: relevance to anhydrobiosis. Biochimica et Biophysica Acta, 1425 (1), 245-254.
• Tikhonova, V. L. (1999). Long-Term Storage of Seeds. Russian Journal of Plant Physiology, 46, 467–476.
• Tribe, D. E. (1994). Feeding and greening the world. The rol of international agricultural research. CABI, Wallingford, Oxon, UK.
• Yücel, E., Duran, A., Türe, C., Böcük, H., Özaydın, B. (2008). Effects of different salt (NaCl), nitrate (KNO3) and acid (HCl and H2S04) concentrations on the germination of some Hesperis species seeds. Biological Diversity and Conservation, 1 (2), 91-104.