Ratlarda 7, 12-DMBA ile oluşturulan kanser modelinde α-lipoik asit ve resveratrol’un etkileri

Year 2015, Volume: 8 Issue: 1, 1 - 6, 15.04.2015

Muammer Bahsı Okkes Yılmaz

Abstract

α-Lipoik asit LA diyabetik polinöropati ve hepatik hasarları kapsayan çeşitli hastalıklarda terapatik ajan olarak yoğun olarak araştırılmıştır. Bununla birlikte LA ve indirgenmiş formu olan dihidrolipoik asit DHLA kanserin, kimyasal yollardan önlenmesi üzerine etkileri rapor edilmiştir. Bu çalışmada DHLA/LA’in 7,12-dimetilbenzentrasen tarafından oluşturulan kanser modeline etkileri araştırılmıştır. Diğer bir terapatik ajan olan resveratrol, antioksidan ve antimutajen olarak dut, üzüm, fıstık, çam ve şarapta yüksek konsatrasyonlarda bulunmuştur. Bunlara ek olarak LA ve resveratrol, ratlarda oluşturulan kanser modelinde kanserojen tedavide preneoplastik lezyonların gelişimini inhibe etmiştir. Bu çalışmada elde edilen veriler, resveratrol ve α-lipoik asitin insanların beslenmelerinde yaygın bir bileşen ve insanlarda kanserin kimyasal olarak önlenmesinde potansiyel bir ajan olduğunu göstermiştir

Keywords

α-Lipoik asit, resveratrol, DMBA, yağ asitleri, vitaminler

Effects of ?-lipoic acid and resveratrol on rats cancer model induced by 7, 12-DMBA

Abstract

α-Lipoic acid LA has been intensely investigated as a therapeutic agent for several diseases, including hepatic disorder and diabetic polyneuropathy. However, the effects of LA or its reduced form, dihydrolipoic acid DHLA , on cancer chemoprevention has been slightly reported. In the present study, we examined the effects of DHLA/LA on rat cancer model induced by 7,12-dimethylbenz a anthracene DMBA . Another therapeutic agent; Resveratrol, was found in high concentrations in berry, grape, peanut, pine and wine and act as an antioxidant and antimutagen. In addition, resveratrol and α-lipoic acid inhibited the development of preneoplastic lesions in carcinogen-treated rat cancer model. Datas in this study suggest that resveratrol and α-Lipoic acid, a common constituent of the human diet, merits investigation as a potential cancer chemopreventive agent in humans

Keywords

α-Lipoic acid, resveratrol, DMBA, fatty acids, vitamins

References

• Anbuselvam, C., Vijayavel, K., Balasubramanian M.P. 2007. Protective effect of Operculina turpethum against 7,12- dimethylbenz(a)anthracene induced oxidative stress with reference to breast cancer in experimental rats. Chem- Bio Int. 168: 229–236.
• Arivazhagan, P., Panneerselvam, S.R., Panneerselvam, C. 2003. Effect of DL-alpha-lipoic acid on the status of lipid peroxidation and lipids in aged rats. J Gerontol A Biol Sci Med Sci. 58: 788–791.
• Bastianetto, S. & Quirion, R. 2010. Heme oxygenase 1: another possible target to explain the neuroprotective action of resveratrol, a multifaceted nutrient-based molecule. Exp Neurol. 225: 237–239.
• Bloch, B. 1921. Drefuss W: Veber die experimentelie erzuegung carcinomen mitlymphdrüsen and iungenmetastagen Durch Teerbestandteile. Schweiz Med, Noschshr. 5: 1033-1037.
• Bobermin, L.D., Quincozes-Santos, A., Guerra, M.C., Leite, M.C., Souza, D.O., Goncalves, C.A., Gottfried, C. 2012. Resveratrol prevents ammonia toxicity in astroglial cells. PLoS One. 7 (12): e52164.
• Brener, R.R. 2000. Effects of experimental diabetes on the fatty acids composition, molecular species of phosphatidylcholina and physical properties of hepatic microsomal membranes. Prost Leuk and Ess F Acids. 63:167-176.
• Çelik, S. & Özkaya, A. 2002. Effects of intraperitoneally administered lipoic acid, vitamin E, and linalool on the level of total lipid and fatty acids in guinea pig brain with oxidative stres induced by H2O2. J of Bioch and Mol Biol. 35(6): 547–52.
• Çelik, S. & Özkaya, A. 2002. Increased unsaturated fatty acid levels in liver and muscle of guinea pig induced by vitamin-E, ALA and linalool. Pak J of Biol Sci. 5(8): 881–884.
• Christie, W.W. 1992. Gas chromatography and lipids. The Oil Pres Glaskow 302.
• Fukui, M., Choi, H.J., Zhu, B.T. 2010. Mechanism for the protective effect of resveratrol against oxidative stress- induced neuronal death. Free Radic Biol Med. 49: 800–813.
• Guyton, A.C. & Hall, J.E. 1996. Textbook of medical physiology. W.B, Sauders Company, Philadelphia.
• Hara, A. & Radin, N.S. 1978. Lipid extraction of tissues with a low-toxicity solvent. Anal Bioch. 90: 420-426.
• Ide, T., Azechi, A., Suzuki N., Kunimatsu, Y., Nakajima, C., Kitade, S. 2013. Effects of dietary a-lipoic acid enantiomers on hepatic fatty acid metabolism in rats. J of func foods. 5: 71-79.
• Jelinska, M., Tokarz, A., Oledzka, R., Alicja, C.S. 2003. Effects of dietary linseed, evening primrose or fish oils on fatty acid and prostaglandin E2 contents in the rat liver and 7,12-dimethylbenz(a)anthracene-induced tumours. Bioch et Biophy Acta (BBA)- mol Basis of Dis. 1637: 193–199.
• Kan, A. 2014. A new plant for Turkey; Buckwheat (Fagopyrum esculentum). Biological Diversity and Conservation. 7/2: 154-158
• Katsanidis, E. & Addis, P.B. 1999. Novel HPLC analysis of tocopherols and cholesterol in tissue. Free Radic Biol Med. 27: 1137-1140.
• Keser, S. 2006. The effect of trans-3,5,4'-trihydroxystilbene on biochemical variations in some tissues of old female rats exposed to effect of potassium bromated. Firat Uni Sci Ins mast thesis, Elazig.
• Khaidakov, M., Bishop, M.E., Manjanatha, M.G., Lyn-Cook, L.E., Desai, V.G.,Chen, J.E., Aidoo, A. 2001. Influence of dietary antioxidants on the mutagenicity of 7,12- dimethylbenz(a)anthracene and bleomycin in female rats. Fund and Mol Mech of Mut. 480–481: 163–170.
• Magdalena, D., Joanna, K., Marek, B., Leszek, N., Małgorzata, Z., Anna, B.W.W., Małgorzata, I., Monika, O., Iwona, Z., Jacek, S., Lidia, W., Barbara, F. 2014. Alpha lipoic acid protects the heart against myocardial post ischemia– reperfusion arrhythmias via KATP channel activation in isolated rat hearts. Pharmacological Reports. 66: 499– 504.
• Mukhopadhyay, S., Rajaratnam, V., Mukherjee, S., Das, S.K. 2008. Control of peripheral benzodiazepine receptor- mediated breast cancer in rats by soy protein. Mol Carcino. 47: 310-319.
• Navari-Izzo, F., Quartacci, M.F., Sgherri, C. 2002. Lipic acid: a unique antioxidant in the detoxification of activated oxygen species. Plant Physiol Biochem. 40: 463–470.
• Ntambi, J.M. 1999. Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal of Lip Res. 40(9): 1549–1558.
• Packer, L. 1998. α-lipoic acid: a metabolic antioxidant which regulates NF-kB signal transduction and protects against oxidative injury. Drug Metab Rev. 30: 245–275.
• Park, S.J., Ahmad, F., Philp, A., Baar, K., Williams, T., Luo, H., Ke, H., Rehmann, H., Taussig, R., Brown, A.L., Kim, M.K., Beaven, M.A., Burgin, A.B., Manganiello, V., Chung, J.H. 2012. Resveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. Cell. 148: 421–433.
• Scholich, H., Murpy, M.E., Sies, H. 1989. Antioxidant activity of dihydrolipoate against microsomal lipid peroxidation and its dependence on alpha-tocopherol. Biochem Biophys Acta. 1001: 256–261.
• Senturk, H., Bayramoglu, G., Dokumacioglu, A., Aral, E., Kanbak, G., İnal, M.,Oglakci A. 2012. Protective effects of Echinacea purpurea in an acetic acid induced rat model of colitis. Biological Diversity and Conservation. 5/2: 59-65
• Shetty, A.K. 2011. Promise of resveratrol for easing status epilepticus and epilepsy. Pharmacol Ther. 131: 269–286.
• Shimaa, M.E., Mohamed, A.E., Waleed, B. 2014. Insights in the mechanism underlying the protective effect of α-lipoic acid against acetaminophen-hepatotoxicity. Euro J of Pharm. 726: 116-123.
• Shimaa, O.A., Hebatallah, A.D., Nabila, A.I. 2014. Modulatory effects of curcumin, silybin-phytosome and alpha-R- lipoic acid against thioacetamide-induced liver cirrhosis in rats. Chem-Biol Int. 216: 26-33.
• Sylvia, H., Robert, D., Longquan, X., Jennifer, R., Witold, W., Oscar, A., Stephen, S., Nobuyo, M., Xianwen, Y. 2014. α-Lipoic acid protects mitochondrial enzymes and attenuates lipopolysaccharide-induced hypothermia in mice. Free Rad Bio and Med. 71: 362-367.
• Yang, C.Z., Casey, W., Stoner, M.A., Kollessery, G.J., Wong, A.W., Bittner, G.D. 2014. A robotic MCF-7:WS8 cell proliferation assay to detect agonist and antagonist estrogenic activity. Toxicol Sci. 137(2): 335-349.
• Zinser, G.M., MeEleney, K., Welsh, J. 2003. Characterization of mammary tumor cell lines type and vitamin D-3 receptor knockout mice. Mol and Cell Endoc. 200 (1–2): 67–80.