Effects of parasitization and envenomation by the endoparasitic Pimpla turionellae on Hemolymph protein profile of its Host Galleria mellonella

Year 2013, Volume: 6 Issue: 1, 62 - 70, 15.04.2013

Ekrem Ergın Hülya Altuntaş Fevzi Uçkan

Abstract

The effects of dose-dependent envenomation and by parasitization of Pimpla turionellae L. Hymenoptera: Ichneumonidae on the hemolymph protein profile of its host Galleria mellonella L. Lepidoptera: Pyralidae were investigated. Hemolymph proteins were analyzed using spectrophotometry and sodium dodecyl sulphate polyacrylamide gel electrophoresis SDS-PAGE . The gel was subsequently scanned and the optical densities OD of the bands were analyzed. The quantities of proteins detected 4, 8, and 24 h post-treatments in hemolymph of parasitized and envenomated host pupae did not differ much when compared with those of controls. Of the seventeen different protein bands detected at a range of 19.6-181.12 kDa in the hemolymph, there were only changes in OD values of bands at 23.418, 24.714, 32.434, 34.811, and 45.385 kDa following envenomation and parasitism. The electrophoretic pattern of hemolymph proteins from venom injected and control groups of larvae did not differ much from that of pupae except for new protein bands detected at 33.823 and 41.553 kDa. However, three bands with 45.385, 99.000, and 126.850 kDa were not detected in larvae. Hemolymph protein quantity remained steady at all time points tested except for increases for some bands at 8 h following envenomation. The amount of 34.811 kDa protein decreased immediately at 8 h postinjection of 0.02 and 0.05 VRE of venom whereas injection all venom doses except 0.1 VRE resulted in an increase in the amount for 41.553 and 43.412 kDa proteins. There were no qualitative changes in term of novel protein bands in the hemolymph of hosts. Therefore, we suggest that host regulation of G. mellonella by parasitism or envenomation of P. turionellae involves quantitative changes in the host plasma proteins but does not lead to the up-regulation of novel proteins

Keywords

Hemolymph proteins, Galleria mellonella, Pimpla turionellae, Venom, Parasitism

Endoparazitik arı Pimpla turionellae L. Hymenoptera: Ichneumonidae ’nın parazitleme ve zehirinin konağı Galleria mellonella L. Lepidoptera: Pyralidae ’nın hemolenf proteinleri üzerine etkisi

Abstract

Pimpla turionellae L. Hymenoptera: Ichneumonidae ’nın farklı dozlardaki zehrinin ve parazitlemesinin konağı Galleria mellonella L. Lepidoptera: Pyralidae ’nın hemolenf proteinleri üzerine olan etkileri araştırıldı. Hemolenf proteinleri spektrofotometrik ve Sodyum Dodesil Sülfat Poliakrilamid Jel Elektroforez SDS-PAGE teknikleri kullanılarak analiz edildi. Jeller tarandıktan sonra, bantların optik densitometrik değerleri analiz edildi. Parazitlenmiş ve zehirle muamele edilen konak pup hemolenfindeki protein miktarları kontrol gruplarıyla karşılaştırıldığında, muameleden 4, 8 ve 24 saat sonrasında değişiklik göstermedi. Hemolenfte belirlenen 19.6-181.12 kDa aralığındaki on yedi farklı proteinden 23.418, 24.714, 32.434, 34.811 ve 45.385 kDa olanların miktarları parazitleme ve zehir etkisine bağlı olarak değişiklik gösterdi. Larvaların hemolenfindeki proteinlerin elektroforetik dağılımlarında hem kontrol grubunda hem de zehir enjekte edilen gruplarda pupa hemolenfinden farklı olarak 33.823 ve 41.553 kDa büyüklüğünde iki yeni protein belirlendi. Bununla birlikte, larvalarda 45.385, 99.000 ve 126.850 kDa büyüklüğündeki üç band belirlenmedi. Larvalarda hemolenf proteinlerinden bazıları enjeksiyondan 8 saat sonrasında artış gösterirken, diğer proteinler tüm zaman noktalarında değişiklik göstermedi. 34.811 kDa’luk proteinin miktarı 0.02 ve 0.05 kese eşdeğeri zehir KEZ dozlarının enjeksiyonundan 8 saat sonra azalma gösterirken 41.553 ve 43.412 kDa büyüklüğündeki proteinler 0.1 kese eşdeğeri zehir KEZ dozu haricindeki tüm dozların enjeksiyonundan sonra artış gösterdi. Konak hemolenfinde zehirleme ve zehir enjeksiyonuna bağlı olarak proteinlerde kalitatif bir değişiklik yani yeni bir protein belirlenmedi. Bu nedenle, P. turionellae parazitleme veya zehirleme yoluyla konak G. mellonella’yı düzenlemesinde konağın plazma proteinlerinde kantitatif değişikliklere neden olduğunu ancak yeni proteinlerin salgılanmasının düzenlenmesinde etkili olmadığını ileri sürmekteyiz

Keywords

Hemolenf Proteinleri, Galleria mellonella, Pimpla turionellae, Zehir, Parazitlik

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