Türkiye için endemik bir türün çekirdek DNA miktarı: Silene sangaria

Year 2013, Volume: 6 Issue: 1, 88 - 92, 15.04.2013

Çiler Meriç Necmettin Güler

Abstract

Türkiye florası için endemik bir tür olan Silene sangaria Coode & Cullen ‘nın çekirdek DNA miktarı flov sitometri yöntemiyle analiz edildi. Bitkiler doğal ortamlarından toplandı ve saksılara ekilerek büyüme kabinine yerleştirildi. Flow sitometri analizi için, genç yapraklar buz üzerinde, bir petri içinde propidium iodid ilaveli MgSO4tamponu içinde parçalandı. Propidium iodid ile boyanan çekirdekler EPICS XL Beckmann Coulter model flov sitometri ile analiz edildi. Silene sangaria’ nın çekirdek DNA miktarı 2C-değeri 4.76 ± 0.20 pg olarak hesaplandı. Türün somatik kromozom sayısı klasik karyolojik metodları kullanılarak belirlendi ve 2n = 4x = 48 olarak sayıldı. Bu çalışmanın angiosperm taksonlarının çekirdek DNA miktarı bilgilerine katkı sağladığı düşünülmektedir

Keywords

Caryophyllaceae, Silene sangaria, Endemik tür, Çekirdek DNA içeriği, Flow sitometri

Nuclear DNA content of an endemic species for Turkey: Silene sangaria

Abstract

The nuclear DNA content of Silene sangaria Coode & Cullen, an endemic species for Turkey, was determined by flow cytometrical analyses techniques. The plant samples were collected from their natural habitats. They transferred into pots and placed in the growth chamber. For flow cytometry analysis, young leaves were chopped in an MgSO4buffer with propidium iodide on ice in a petri dish. Nuclei, which were stained with propidium iodide, were analyzed on an EPICS XL model flow cytometer. The nuclear DNA content 2C-value of S. sangaria was found to be 4.76 ± 0.20 pg. The somatic chromosome number of the species was determined using conventional karyological methods and counted as 2n = 4x = 48. This study contributes to the data on the nuclear DNA content of angiosperm taxa

Keywords

Caryophyllaceae, Silene sangaria, Endemic species, Nuclear DNA content, Flow cytometry

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