The determination of genetic relationships among someVicia L. Vetch taxa by using ISSR markers

Year 2013, Volume: 6 Issue: 3, 135 - 139, 15.12.2013

Meryem Bozkurt Kuddisi Ertuğrul Tuna Uysal

Abstract

The genetic relationship among some naturally growing Vicia taxa in Turkey V. sativa subsp. sativa, subsp.nigra subspstenophylla, V. hybrida, V. peregrinaandV. palaestina was assessed by helping ISSR markers. During PCR assays, although 17 primers were tested, only three primers produced informatics amplification yields for all studied taxa. These ISSR primers had considerably high number of polymorphic locus. According to the dendogram obtained from ISSR profiles, all studied taxa were separated into two main clusters; one of them consists of the section Vicia and the other section Cracca. While the section Vicia comprises the subspecies of V. sativa, V. hybridaand V. peregrina, the section Cracca involve in subgroupsof V. cracca and V. palaestina

Keywords

Vicia, vetch, ISSR markers, Turkey

ISSR markırlarıkullanılarakbazıVicia L. Fiğ taksonlarıarasındakigenetikakrabalıklarınbelirlenmesi

Abstract

Bu çalışmada, Türkiye’de doğal olarak yetişen bazı Vicia taksonları V. sativa subsp. sativa, subsp. nigra var. nigra, subsp incisa var. cordata, V. cracca subsp. cracca, subsp gerardii, subsp atroviolacea, subsp stenophylla, V. hybrida, V.peregrina ve V. palaestina arasındaki genetik akrabalıklar ISSR markırları yardımıyla değerlendirilmiştir. PCR denemeleri sırasında, 17 primer test edilmesine rağmen, sadece üç primer çalışılan tüm taksonlar için bilgi verici amplifikasyon ürünleri vermiştir. Seçilen ISSR primerleri tüm taksonlarda oldukça yüksek sayıda polimorfik lokus oluşturmuştur. ISSR bantlarından elde edilen dendrograma göre, incelenen taksonlardan Vicia seksiyonuna ait olanlar ile Cracca seksiyonuna ait olanlar iki alt grup olarak ayrılmıştır. Vicia seksiyonu alt kümesi, V. sativa’nın tür altı taksonları ile V. hybrida ve V. peregrina türlerinden; Cracca seksiyonu alt kümesi ise V. cracca’nın alt türleri ile V. palaestina türünden oluşmaktadır

Keywords

Vicia, fiğ, ISSR markır, Türkiye

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