Time dependent cytotoxic role of Homalothecium sericeum extracts on glioma

Year 2012, Volume: 5 Issue: 1, 1 - 4, 15.04.2012

Pinar Oztopcuvatan Selda Kabadere Ruhi Uyar Filiz Savaroglu Gökhan Kus

Abstract

Bryophytes have been used as medicinal plants for more than 400 years in China, Europe and North America. There is also evidence confirming the antibiotic and anticancer activity of Bryophytes against, prokaryotes, fungi and different cancer cells. The purpose of the current study was to investigate cytotoxic property of Homalothecium sericeum hedw. schimp., which is a bryophyte, extracts on rat glioma C6 cells for 48 hrs, in vitro. We first collected two different acetone and A extracts from H. sericeum by two different extraction processes. C6 cells were seeded in 96 well plates 2x104 cells/well and incubated for 24 hrs. Following this incubation period, the medium was replaced with only medium control or medium with extracts at concentrations of 0.17, 1.7, 17, 85 or 170 µg/mL for 48 hrs. Cytotoxicity was determined by using 3- 4,5-dimethylthiazol-2yl - 2,5-diphenyltetrazolium bromide MTT colorimetric assay. Acetone extract of H. sericeum at 0.17, 1.7 and 17 µg/mL concentrations did not change the survival rate of C6, but 85 and 170 µg/mL inhibited about 16 % and 36 % after 48 hr p

Keywords

Homalothecium sericeum, Bryophyta, Glioma, MTT, Cytotoxicity

Homalothecium sericeum özütlerinin zamana bağlı olarak glioma hücrelerinin çoğalması üzerindeki etkileri

Abstract

Bryofitler 400 yıldan fazla bir süredir Çin, Avrupa ve Kuzey Amerika’da bitkisel ilaç olarak kullanılmaktadır. Bryofitlerin prokaryot, mantar türleri üzerinde antibiyotik ve farklı kanser hücreleri üzerinde antikanser etkinlik gösterdiği konusunda veriler bulunmaktadır. Bu çalışmanın amacı, bir Bryofit olan Homalothecium sericeum’un sıçan glioma C6 hücreleri üzerinde 48 saatlik sitotoksik etkinliğini araştırmaktır. İlk olarak, iki farklı özüt elde etme yöntemiyle aseton ve A özütlerini elde ettik. C6 hücreleri 96 kuyucuklu kültür kaplarına ekilerek 2x104 hücre/kuyucuk 24 saat inkübe edildi. İnkübasyon süresinin ardından kuyucuklara özütlerin 0.17, 1.7, 17, 85 ve 170 µg/mL konsantrasyonları eklenerek 48 saat muamele edildi. Sitotoksisite, MTT [3- 4,5-dimetiltiazol-2yl - 2,5difeniltetrazolyum bromid] yöntemi ile belirlendi. 48 saat sonunda aseton özütünün 0.17, 1.7 ve 17 µg/mL konsantrasyonları C6 hücre canlılığını değiştirmezken, 85 ve 170 µg/mL konsantrasyonları hücre canlılığını sırası ile %16 ve %36 oranında azalttı p

Keywords

Homalothecium sericeum, Bryofit, Glioma, MTT, Sitotoksisite

References

• Abe, K., Matsuki, N., 2000. Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT. Neuroscience Research. 38: 325-29.
• Asakawa, Y. 2001. Recent advances in phytochemistry of bryophytes-acetogenins, terpenoids and bis (bibenzyl)s from selected Japanese, Taiwanese, New Zealand, Argentinean and European liverworts. Phytochemistry. 56: 297-312.
• Basile, A., Sorbo, S., Giordano, S., Lavitola, A., Castaldo-Cobianchi, R. 1998. Antibacterial activity in Pleurochaete squarrosa extract (Bryophyta). International Journal of Antimicrobial Agents. 10: 169-172.
• Basile, A., Giordano, S., Lopez-Saez, J.A., Castaldo-Cobianchi, R. 1999. Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry. 52: 1479-1482.
• Collet, B., Guitton, N., Saikali, S., Avril, T., Pineau, C., Hamlat, A., Mosser, J., Quillien, V. 2011. Differential analysis of glioblastoma multiforme proteome by a 2D-DIGE approach. Proteome Science. 9/1: 1-26.
• Ezer, T., Kara, R., Düzenli, A. 2010. The life strategies of bryophytes which form epiphytic vegetation on Mount Musa/Turkey. Biological Diversity and Conservation. 3/3: 75-84.
• Hahn, H., Seeger, T., Geiger, H., Zinsmeister, H.Z., Markham, K.R., Wong, H. 1995. The first biaurone, a triflavone and biflavonoids from two Aulacomnium species. Phytochemistry. 40: 573-576.
• Ivanova, V., Kolarova, M., Aleksieva, K., Dornberger, K.J., Haertl, A.., Moellmann, U., Dahse, H.M., Chipev, N., 2007. Sanionins: Anti-inflammatory and antibacterial agents weak cytotoxicity from the Antarctic Moss Sanionia georgico-uncinata. Preparative Biochemistry and Biotechnology. 37: 343-352.
• Krzaczkowski, L., Wright, M., Reberioux, D., Massiot, G., Etievant, C., Gairin, J.E. 2009. Pharmacological screening of bryophyte extracts that inhibit growth and induce abnormal phenotypes in human HeLa cancer cells. Fundamental & Clinical Pharmacology. 23/4: 473-482.
• Liu, K.W., Feng, H., Bachoo, R., Kazlauskas, A., Smith, E.M., Symes, K., Hamilton, R.L., Nagane, M., Nishikawa, R., Hu, B., Cheng, S.Y. 2011. SHP-2/PTPN11 mediates gliomagenesis driven by PDGFRA and INK4A/ARF aberrations in mice and humans. The Journal of Clinical Investigation. 121/3: 905-917.
• Matsuo, A., Sato, A. 1991. Sterols of Mosses. Phytochemistry. 30: 2305-2306.
• Mossmann, T. 1983. Rapid colorimetric assay of cellular growth and survival: application to proliferation and cytotoxicity assay. Journal Immunology Method. 65: 55-63.
• Oztopcu-Vatan, P., Savaroglu, F., Filik-Iscen, C., Kabadere, S., Ilhan, S., Uyar, R. 2011. Antimicrobial and antiproliferative activities of Homalothecium sericeum (Hedw.) Schimp. extracts. Phresenius Environmental Bulletin. 20: 461-466.
• Sabovljević, A., Soković, M., Sabovljević, M., Grubišić, D. 2006. Antimicrobial activity of Bryum argenteum. Fitoterapia. 77: 144-145.
• Salford, L.G., Siesjö, P., Skagerberg, G., Persson, B.R.R., Larsson, E.M., Lindvall, M., Visse, E., Widegren, B. 2002. Search for effective therapy against glioblastoma multiforme-clinical immunisation with autologous glioma cells transduced with the human interferon-g gene. International Congress Series. 1247: 211-220.
• Saritas, Y., Sonwa, M.M., Iznaguen, H., König, W.A., Muhle, H., Mues, R. 2001. Volatile constituents in mosses (Musci). Phytochemistry. 57: 443-457.
• Savaroglu, F., Filik-Iscen, C., Oztopcu-Vatan, P., Kabadere, S., Ilhan, S., Uyar, R. 2011.Determination of antimicrobial and antiproliferative activities of the aquatic moss Fontinalis antipyretica Hedw. Turkish Journal of Biology. 35: 361-369.
• Singh, M., Rawat, A.K.S., Govindarajan, R. 2007. Antimicrobial activity of some Indian mosses. Fitoterapia. 78: 156- 158.
• Yamada, P., Isoda, H., Han, J.K., Talorete, T.P., Yamaguchi, T., Abe, Y. 2007. Inhibitory effect of fulvic acid extracted from Canadian sphagnum peat on chemical mediator release by RBL-2H3 and KU812 cells. Bioscience, Biotechnology and Biochemistry. 71: 1294-1305.
• Zinsmeister, H.D., Mues, R. 1987. Moose als Reservoir bemerkenswerter sekundaerer Inhaltsstoffe. GIT Fachzeitscchrift für das Laboratorium. Gıt Verlag. 31: 499-512.
• Zinsmeister, H.D., Becker, H., Eicher, T. 1991. Bryophytes, a source of biologically active, naturally occurring material. Angewandte Chemie-International Edition in English. 30: 130-147.