Karyological study on endemic Astragalus stereocalyx Bornm. Milk-vetch in Turkey

Year 2010, Volume: 3 Issue: 3, 153 - 157, 15.12.2010

Özlem Çetin Esra Martin Ahmet Duran Ayşe Özdemir

Abstract

Chromosome number and karyotype of Astragalus stereocalyx Bornm. Milk-vetch in the section Stereocalyx Bornm. distributing naturally in Turkey were analyzed in detail. This species is endemic to Turkey and growing in Eskişehir and Konya provinces. Squash preparation method was used for chromosome study in this species. The somatic chromosome number was determined as 2n = 16 in A. stereocalyx. The basic chromosome number was observed as x = 8. The chromosome number and morphology of A. stereocalyx belonging to the genus Astragalus was investigated using Image Analysis System. The karyotype formula of this species consists of three median chromosome pairs and five submedian chromosome pairs. The somatic chromosome length between 1.07-2.23 µm. Total haploid chromosomes length was measured as 11.73 µm. The karyogram and ideogram were drawn based on centromeric index and arranged in the decreasing size order

Keywords

Astragalus stereocalyx, Karyotype, Leguminosae, Turkey

Türkiye’deki endemik Astragalus stereocalyx Bornm. Geven türünde karyolojik çalışma

Abstract

Türkiye’de doğal olarak yetişen Stereocalyx Bornm. seksiyonunda yer alan Astragalus stereocalyx Bornm. Geven türünde kromozom sayı ve karyotipi detaylı olarak analiz edildi. Bu tür Türkiye için endemiktir, Eskişehir ve Konya illerinde yetişmektedir. Bu türün kromozom çalışmasında ezme yayma preparasyon yöntemi kullanıldı. A. stereocalyx türünde somatik kromozom sayısı 2n = 16 olarak belirlendi. Temel kromozom sayısı x = 8 şeklinde gözlendi. Astragalus cinsine ait olan A. stereocalyx’in kromozom sayı ve morfolojisi Görüntü Analiz Sistemi kullanılarak araştırıldı. Türün karyotip formülü üç median ve beş submedian kromozom tiplerinden oluşmaktadır. Somatik kromozom boyu 1.07-2.23 µm arasındadır. Toplam haploid kromozom uzunluğu 11.73 µm olarak ölçüldü. Karyogram ve idiyogramı sentromerik indeks ve büyükten küçüğe doğru azalan oranda çizildi

Keywords

Astragalus stereocalyx, Karyotip, Leguminosae, Türkiye

References

• Açık, L., Ekici, M., Çelebi, A. 2004. Taxonomic relationships in Astragalus sections Hololeuce and Synochreati (Fabaceae): Evidence from RAPD-PCR and SDS-PAGE of seed proteins. Annales Botanici Fennici. 41. 305-317.
• Akan, H., Aytaç, Z. 2004. Astragalus ovabaghensis (Fabaceae), a new species from Turkey. Annales Botanici Fennici. 41. 209-212.
• Aytaç, Z. 1997. The revision of the Section Dasyphyllium Bunge of the genus Astragalus L. of Turkey. Turkish Journal of Botany. 21. 31-57.
• Chamberlain, D. F., Matthews, V. A. 1970. Astragalus L. In: Flora of Turkey and the East Aegean Islands. Davis PH (Ed.). Edinburgh University Press, Edinburgh. 3. 249-254.
• Davis, P. H., Mill, R. R., Tan, K. 1988. Astragalus L. In: Flora of Turkey and the East Aegean Islands (Supplement). Davis, P. H., Mill, R. R., Tan, K. (Ed.). Edinburgh University Press, Edinburgh, 10. 114-124.
• Duman, H., Akan, H. 2003. New species of Astragalus (sect. Alopecuroidei: Leguminosae) from Turkey. Botanical Journal of the Linnean Society. 143. 201-205.
• Dural, H., Tugay, O., Ertuğrul, K., Uysal, T., Demirelma, H. 2007. Astragalus turkmenensis (Fabaceae), a new species from Turkey. Annales Botanici Fennici. 44. 399-402.
• Duran, A., Aytaç Z. 2005. Astragalus nezaketae (Fabaceae), a new species from Turkey. Annales Botanici Fennici. 42. 381-385.
• Duran A., Martin E., Öztürk, M., Çetin,Ö., Dinç, M., Özdemir, A. Morphological, karyological and ecological features of halophytic endemic Sphaerophysa kotschyana (Leguminosae) in Turkey. Biological Diversity and Conservation. 3. 163-169.
• Ekici, M., Aytaç, Z. 2001. Astragalus dumanii (Fabaceae), a new species from Anatolia, Turkey. Annales Botanici Fennici. 38. 171-174.
• Ekici, M., Yüzbaşıoğlu, D., Aytaç, Z. 2005. Morphology, pollen, seed structure and karyological study on Astragalus ovalis Boiss. and Balansa (Sect. Ammodendron) in Turkey. International Journal of Botany. 1. 74-78.
• Ghahremaninejad, F., Behçet, L. 2003. Astragalus subhanensis (Fabaceae), a new species from Turkey. Annales Botanici Fennici. 40. 209-211.
• Hamzaoğlu, E. 2003. Astragalus hamzae (Fabaceae), a new species from Central Anatolia, Turkey. Annales Botanici Fennici. 40. 291-294.
• Kandemir, N., Korkmaz, H., Engin, A. 1996. The Morphological and Anatomical Properties of Astragalus barba-jovis DC. var. barba-jovis (Fabaceae). Turkish Journal of Botany. 20. 291-299.
• Kandemir, N. 2010. A karyological investigation on the two varieties of Galanthus fosteri Baker (Amaryllidaceae). Biological Diversity and Conservation. 2: 20-25.
• Karagöz, A., Turgut-Kara, N., Çakır, Ö., Demirgan, R., Arı, Ş. 2007. Cytotoxic activity of crude extracts from Astragalus chrysochlorus (Leguminosae). Biotechnology & Biotechnological Equipment. 220-222.
• Levan, A., Fredga, K., Sandberg, AA. 1964. Nomenclature for centromeric position on chromosomes. Hereditas. 52: 201-220.
• Madadi, R., Asghari-Zakaria, R., Fathi, M. 2009. Karyotype study in several populations of Papaver dubium from North West of Iran. Biological Diversity and Conservation. 2: 18-22.
• Martin, E., Duran, A., Dinç, M., Erişen, S., Babaoğlu, M.2008. Karyotype Analyses of four Astragalus L. (Fabaceae) species from Turkey. Phytologia. 90. 133-146.
• Podlech, D. 1986. Taxonomic and phytogeographical problems in Astragalus of the Old World and South West Asia. Proceedings of the Royal Society. 89. 37-43.
• Podlech, D. 1999. New Astragali and Oxytropis from North Africa and Asia, including some new combinations and remarks on some species. Sendtnera. 6. 135-171.
• Spellenberg, R. 1976. Chromosome numbers and their cytotaxomic significance for North American Astragalus (Fabaceae). Taxon. 25. 463-476.
• Tang, W. 1992. Chinese Drugs of Plant Origin. Springer Verlag, Berlin. 191-197.