Genetic variation of high yielding drought resistant sweet potato as evident by RAPD markers

Year 2008, Volume: 1 Issue: 1, 28 - 39, 01.01.2008

Anath Bandhu Das Samir Kanti Naskar

Abstract

Detailed agronomical analysis and random amplified polymorphic RAPD markers were used for determining the genetic diversity among ten varieties of sweet potato Ipomoea batatas L. Lam , one of the leading tuber crop belonging to the family Convolvulaceae. Varieties developed by Central Tuber Crops Institute, Trivandrum and its Regional Centre, Bhubaneswar with diverse parentage from diverse eco-geographical areas was analyzed for their genetic relationship. A total 1035 amplicons were generated among the ten varieties out of which ~79% bands were found polymorphic. Inter-varietal polymorphism among ten varieties of I. batatas varied between 60.0 to 89.7%. Clustering based on similarity index was done following Unweighted Pair Group with Arithmetic mean UPGA method and intra-genetic relationships were analysed. The resultant dendrogram of the RAPD data exhibited prominent patterns of inter-varietal relationships that are discussed in the light of the their physio-morphological characters. It was evident from RAPD data that high degree of genetic divergence exists only in var. Kalinga and var. Sourin; two pink skinned tuber producing varieties. However, no much genetic variation were found among var. ST-10, ST-13 and ST-14 except presence of 3000bp marker in ‘ST-14’ and 800bp marker in ‘ST-14’ and ‘ST-13’ besides their common marker of 900bp in all the studied varieties in OPD-12. Omission of 600bp and 700bp DNA bands in ‘ST-10’ differentiate with other ‘ST-13’ and ‘ST-14’ in OPN-4. ‘Kalinga’ and ‘Sourin’ showed more closer genetic affinity forming an out group from the rest of the varieties as per the phylogenetic tree is concerned. It suggests their adaptation in various conditions suitable for the specific habitat of particular varieties

Keywords

Agronomic traits, Ipomea, RAPD markers, tuber crop, tuber yield

References

• Bovell-Benjamin, A.C. 2007. Sweet Potato: A review of its past. Present and future role in human nutrition, In (Ed.) Taylor, S.L., Advances in Food and Nutrition Research, Academic Press, UK, pp 1-48.
• Buteler, M. I., Jarret, R. L., La Bonte, D. R. 1999. Sequence characterization of microsatellite in diploid and polyploid Ipomoea. Theor. Appl. Genet. 99. 123-132.
• Connolly, A. G., Godwin, M., Cooper, I. D., Delacy, I. H. 1994. Interpretation of randomly amplified polymorphic DNA marker data for fingerprinting Sweet potato (Ipomoea batatas L.) genotypes. Theor. Appl. Genet. 88. 332-336.
• Dawson, I. K., Chalmers, K. J., Waugh, R., Powell, W. 1993. Detection and analysis of genetic variation in Hordeum spontaneum population from Israel using RAPD markers. Mol. Eco. 3. 151-159.
• Dhillon, N. P. S., Ishiki, K. 1999. Genomic variation and genetic relationships in Ipomoea spp. Plant Breeding 118. 161- 165.
• Dizon, A. E., Lockyer, C., Perrin, W. F., DeMaster, D. P., Sisson, J. 1992. Rethinking the stock concept: aphylogeogr aphic approach. Conser. Biol. 6. 24-36.
• Gichuki, S. T. 2003.Genetic diversity in sweet potato in relationship to geographic sources as assessed with RAPD markers. Genetic Resources and Crop Evolution. 50. 429-437.
• He, G., Prakash, C. S., Jarret, R. L. 1995. Analysis of genetic diversity in a sweet potato (Ipomoea batatas) germplasm collection using DNA amplification fingerprinting. Genome. 38. 938-945.
• Jarret R. L., Austin, D. F. 1994. Genetic diversity and systematic relationships in sweet potato (Ipomoea batatas (L.) Lam) and related species as revealed by RAPD analysis. Genet. Resour. Crop Evl. 41. 165-173.
• Jones, A., Deonier, M. T. 1965. Interspecific crosses among Ipomoea ramoni, Ipomoea trichocarpa and Ipomoea triloba. Botanical Gazette. 126. 226-232.
• Naskar, S. K., Mukherjee, A., Ray, R. C., Moorthy, S. N. 2007. Breeding sweet potato: value addition for food, feed and industrial use. Proceedings NSRTC-2, 2005, CTCRI, Thiruvananthapuram, Kerala, India. pp. 308-314.
• Nei, M. 1975. Molecular population genetics and evolution. American Elsevier, NewYork.
• Rohlf, F. J. 1993. Ntsys-PC. Numerical taxonomy and multivariate analysis system Version 1. 80-Setauket, NY, Exeter Software.
• Sagredo, B., Hinrichsen, P., Lopez, H. , Cubillos, A., Munoz, C. 1998. Genetic variation of sweet potatoes (Ipomoea batatas L.) cultivated in Chile determined by RAPDs. Euphytica 10. 193-198.
• Tring, Y. C., Kehr, A. E., Miller, J. C. 1957. A cytological study of the sweet potato plant Ipomoea batatas (L.) Lam. and its related species. The American Naturalist. 91. 197-203.
• Tioutine M. G. 1935. Breeding and selection of sweet potatoes. J. Hered. 26. 3-10.
• Waples, R. S. 1995. Evolutionary significant units and the conservation of biological diversity under the endangered species act, In (Ed.) Nielsen, J. L., Evolution and the aquatic ecosystem: defining unique units in population conservation. American Fisheries Society, Bethesda MD. Pp 8-27.
• Williams, J. G. K., Kulelik, A. R., Liver, J., Rafalski, A., Tingey, S. V., 1990. DNA polymorphism identification by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18. 6531-6535
• Zhang, D. P., Ghislain, M. , Huaman, Z., Golmirzaie, A., Hijmans, R. 1998. RAPD variation in sweet potato cultivars from South America and Papua New Guinea. Genetic Resources and Crop Evolution. 45. 271-277.
• Zhang, D., Cervantes, J., Huamán, Z., Carey, E., Ghislain, M. 2000. Assessing Genetic Diversity of sweet potato (Ipomoea batatas (L.) Lam.) cultivars from tropical America using AFLP. Genetic Resources and Crop Evolution. 47/6. 659-665.