Research Article
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Anti-oxidative and apoptotic properties of curcumin as a mechanism of its antineoplastic potential in U251 cells

Year 2021, Volume 14, Issue 2, 258 - 263, 15.08.2021
https://doi.org/10.46309/biodicon.2021.958768

Abstract

Glioblastoma is the most aggressive of all types of brain cancer. Especially in cancer research, the effectiveness of new drug candidates is being investigated with in vitro studies. In this study, we aimed to investigate the antineoplastic effects of curcumin administration at different doses on U251 cells. We hypothesize that curcumin inhibits U251 cell viability by modulating prooxidant-antioxidant mechanisms, triggering ROS production, apoptosis activity, and inflammation. In this study, IL-1β, TNF-α, caspase 3/9 levels, total antioxidant (TAS) and total oxidant levels (TOS) were measured. Cell viability was determined by MTT and 10, 20 and 40 µM curcumin doses were found to be effective doses. It was found that the administration of 40 µM curcumin induced oxidative stress, inflammation and apoptosis compared to the control group and other dose groups (p<0.05). No statistical difference was found in the 10 µM curcumin group. We found that 40 µM curcumin had a dose-dependent effect on proliferation and migration of U251 glioblastoma cells and significantly inhibited their proliferation.

References

  • [1] Ostrom, Q. T., Bauchet, L., Davis, F. G., Deltour, I., Fisher, J. L., Langer, C. E., ... & Barnholtz-Sloan, J. S. (2014). The epidemiology of glioma in adults: a “state of the science” review. Neuro-oncology, 16(7), 896-913.
  • [2] Vitovcova, B., Skarkova, V., Rudolf, K., & Rudolf, E. (2020). Biology of Glioblastoma Multiforme—Exploration of Mitotic Catastrophe as a Potential Treatment Modality. International Journal of Molecular Sciences, 21(15), 5324.

Kurkumin’in U251 hücrelerinde antineoplastik potansiyellerinin mekanizması olarak anti-oksidatif ve apoptotik özellikleri

Year 2021, Volume 14, Issue 2, 258 - 263, 15.08.2021
https://doi.org/10.46309/biodicon.2021.958768

Abstract

Glioblastoma beyin kanser türleri arasında en agresif olanıdır. Özellikle kanser araştırmalarında in vitro çalışmalar ile yeni ilaç adaylarının etkinliği araştırılmaktadır. Bu çalışma ile U251 hücreleri üzerinde farklı dozlarda kurkumin uygulamasının antineoplastik etkilerini araştırmayı amaçladık. Kurkumin’in, prooksidan-antioksidan mekanizmaları modüle ederek ROS üretimini, apoptoz aktivitesini ve inflamasyonu tetikleyerek U251 hücre canlılığını inhibe ettiğini varsayıyoruz. Bu çalışmada, IL-1β, TNF-α, kaspaz 3/9 seviyeleri, toplam antioksidan (TAS) ve toplam oksidan seviyeleri (TOS) ölçüldü. MTT ile hücre canlılıkları belirlendi ve 10, 20 ve 40 µM kurkumin dozlarının efektif dozlar olduğu tespit edildi. 40 µM kurkumin uygulamasının kontrol grubu ve diğer doz grupları ile karşılaştırıldığında oksidatif stresi, inflamasyonu ve apoptozu indüklediği bulundu (p<0.05). 10 µM kurkumin grubunda istatistiksel olarak fark tespit edilmedi. 40 µM kurkuminin U251 glioblastoma hücrelerinin proliferasyonu ve migrasyonu üzerinde doza bağlı bir etkiye sahip olduğunu ve proliferasyonunu önemli ölçüde inhibe ettiğini bulduk

References

  • [1] Ostrom, Q. T., Bauchet, L., Davis, F. G., Deltour, I., Fisher, J. L., Langer, C. E., ... & Barnholtz-Sloan, J. S. (2014). The epidemiology of glioma in adults: a “state of the science” review. Neuro-oncology, 16(7), 896-913.
  • [2] Vitovcova, B., Skarkova, V., Rudolf, K., & Rudolf, E. (2020). Biology of Glioblastoma Multiforme—Exploration of Mitotic Catastrophe as a Potential Treatment Modality. International Journal of Molecular Sciences, 21(15), 5324.

Details

Primary Language Turkish
Subjects Biochemistry and Molecular Biology
Journal Section Research Article
Authors

Fatih KAR (Primary Author)
Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Eskişehir, Türkiye
0000-0001-8356-9806
Türkiye


Ceyhan HACIOĞLU
DUZCE UNIVERSITY
0000-0002-0993-6118
Türkiye

Publication Date August 15, 2021
Application Date June 28, 2021
Acceptance Date July 21, 2021
Published in Issue Year 2021, Volume 14, Issue 2

Cite

APA Kar, F. & Hacıoğlu, C. (2021). Kurkumin’in U251 hücrelerinde antineoplastik potansiyellerinin mekanizması olarak anti-oksidatif ve apoptotik özellikleri . Biyolojik Çeşitlilik ve Koruma , 14 (2) , 258-263 . DOI: 10.46309/biodicon.2021.958768

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❖ Biological Diversity and Conservation/ Biyolojik Çeşitlilik ve Koruma
❖ ISSN 1308-5301 Print; ISSN 1308-8084 Online
❖ Start Date Published 2008
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