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Erodium hoefftianum (Geraniaceae)’un Doku Kültürü Yöntemleriyle Üretiminin Araştırılması

Year 2021, Volume 14, Issue 3, 418 - 426, 15.12.2021

Abstract

Bu yeni yaklaşım, doğrudan organogenesis yoluyla Erodium hoefftianum'un üretim yöntemini belirlemek için tasarlanmıştır. Eksplant olarak Murashige ve Skoog (MS) besin ortamında çimlenen tohumlardan elde edilen yan meristemler kullanılmıştır. Daha sonra, ayrı ayrı 1.0/0.1 mg/L zeatin (ZEA)/indol-3- bütirik asit (IBA), 1.0/0.1 mg/L kinetin (KIN)/IBA, 1.0/0.1 mg/L N6-[2-isopentenil]adenin (2iP)/IBA, 1.0/0.1 mg/L 6- Benziladenin (6-BA)/IBA, 1.0/0.1 mg/L thidiazuron (TDZ)/IBA ve bitki büyüme düzenleyicisi olmadan desteklenen MS besin ortamı etkin sürgün çoğalması için araştırılmıştır. 1.0/0.1 mg/L 6-BA/IBA kombinasyonu sürgün uzunluğu hariç tüm durumlarda en uygun bitki büyüme düzenleyicileri olarak bulundu. Zeatin/IBA kombinasyonu 37.28 mm ile sürgün uzunluğu için en ideal olarak belirlendi. Köklenme kabiliyeti ayrıca IBA, indol-3-bütirik asit (IAA) ve 1-naftalen asetik asit (NAA) (0,5 mg/L) veya oksinsiz (kontrol) ile MS kullanılarak da incelenmiştir. Kontrol grubu, %100 köklenme yüzdesi ile tüm durumlarda en yüksek değerlere sahiptir. Turba: perlit (2:1) ortamlarına nakledilen köklü bitkicikler daha sonra iklim odası koşulları altında iklimlendirildi ve botanik bahçesi koşullarına transfer edildi.

References

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  • [3] Fiz, O., Vargas, P., Alarcon, M. L. & Aldasoro, J. J. (2006). Phylogenetic relationships and evolution in Erodium (Geraniaceae) based on trnL-trnF sequences. Systematic Botany, 31, 739-763. https://doi: 10.1600/036364406779695906
  • [4] Messing, S. & Byrne, R. (1998). Premission invasion of Erodium cicutarium in California. Journal of Biogeography, 25, 757-762. https://doi: 10.1046/j.1365-2699.1998.2540757.x
  • [5] Fiz-Palacios, O., Vargas, P., Vila, R., Papadopulos, A. S. & Aldasoro, J. J. (2010). The uneven phylogeny and biogeography of Erodium (Geraniaceae): radiations in the Mediterranean and recent recurrent intercontinental colonization. Annals of Botany, 106(6), 871-884. https://doi: 10.1093/aob/mcq184
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  • [17] Molares, S. & Ladio, A. (2009). Ethnobotanical review of the Mapuche medicinal flora: use patterns on a regional scale. Journal of ethnopharmacology, 122(2), 251-260. https://doi: 10.1016/j.jep.2009.01.003
  • [18] Munekata, P. E., Alcántara, C., Collado, M. C., Garcia-Perez, J. V., Saraiva, J. A., Lopes, R. P., Barba, F. J., Silva, L. P., Sant'Ana, A. S., Fierro, E. M. & Lorenzo, J. M. (2019). Ethnopharmacology, phytochemistry and biological activity of Erodium species: A review. Food research international, 126, 108659. https://doi: 10.1016/j.foodres.2019.108659
  • [19] Mosaddegh, M., Naghibi, F., Moazzeni, H., Pirani, A. & Esmaeili, S. (2012). Ethnobotanical survey of herbal remedies traditionally used in Kohghiluyeh va Boyer Ahmad province of Iran. Journal of ethnopharmacology, 141(1), 80-95. https://doi: 10.1016/j.jep.2012.02.004
  • [20] Rehman, K., Khan, M. A., Ullah, Z. & Chaudhary, H. J. (2015). An ethno botanical perspective of traditional medicinal plants from the Khattak tribe of Chonthra Karak, Pakistan. Journal of ethnopharmacology, 165, 251-259. https://doi: 10.1016/j.jep.2015.02.035
  • [21] Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. & Adıgüzel, N. (2000). Türkiye Bitkileri Kırmızı Kitabı. Ankara: Türkiye Tabiatını Koruma Derneği.
  • [22] Ostrolucká, M. G., Libiakov,á G., Ondrušková, E. & Gajdošová, A. (2004). In vitro propagation of Vaccinium species. Acta Universitatis Latviensis, 676, 207-212.
  • [23] Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi: 10.1111/j.1399-3054.1962.tb08052.x
  • [24] Lambardi, M., Sharma, K. K. & Thorpe, T. A. (1993). Optimization of in vitro bud induction and plantlet formation from mature embryos of Aleppo pine (Pinus halepensis Mill.). In Vitro Cellular & Developmental Biology – Plant, 29, 189-199.
  • [25] Koc, M., Hamzaoğlu, E. & Özhatay, F. N. (2014). Arenaria microcalyx (Caryophyllaceae), a new species from Turkey. Turkish Journal of Botany, 38(4), 653-657. https://doi: 10.3906/bot-1311-27
  • [26] Corral, P., Mallon, R., Oubina-Rodriguez, J. & Gonzalez, M. L. (2011). Multiple shoot ınduction and plant regeneration of the endengered species Crepis novoana. Plant Cell Tissue and Organ Culture, 105, 211-217. https://doi: 10.1007/s11240-010-9854-x
  • [27] Lemay, M., De Vriendt, L., Pellerin, S. & Poulin, M. (2015). Ex situ germination as a method for seed viability assessment in a peatland orchid, Plantanthera blephariglottis. American Journal of Botany, 102, 390-395. https://doi: 10.3732/ajb.1400441
  • [28] Zhang, T., Cao, Z. Y. & Wang, X. Y. (2005). Induction of somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants of Eruca sativa Mill. In Vitro Cellular & Developmental Biology-Plant, 41(5), 655-657. https://doi: 10.1079/IVP2005653
  • [29] Cüce, M. & Sökmen, A. (2017). In vitro production protocol of Vaccinium uliginosum L. (bog bilberry) growing in the Turkish flora. Turkish Journal of Agriculture and Forestry, 41, 294-304. https://doi: 10.3906/tar-1704-19
  • [30] Bekircan, T., Yaşar, A., Yıldırım, S., Sökmen, M. & Sökmen, A. (2018). Effect of cytokinins on in vitro multiplication, volatiles composition and rosmarinic acid content of Thymus leucotrichus Hal. shoots. 3 Biotech, 8(3), 180. https://doi: 10.1007/s13205-018-1206-2
  • [31] Cüce, M., Bekircan, T., Laghari, A. H., Sökmen, M., Sökmen, A., Önay Uçar, E. & Kılıç, A. O. (2019). Phenolic profiles, antimicrobial and cytotoxic properties of both micropropagated and naturally growing plantlets of Calamintha sylvatica subsp. sylvatica Bromf. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(4), 1145-1152. https://doi: 10.15835/nbha47411474
  • [32] Akın, B., Kocaçalışkan, İ. & Güleryüz, G. (2014). Micropropagation of Erodium sibthorpianum subsp. sibthorpianum, an endemic threatened species of Uludağ Mountain (Bursa-Turkey). Turkish Journal of Botany, 38(1), 148-155. https://doi: 10.3906/bot-1304-24
  • [33] Çetin, B., Eren, H., Oskay, D. & Bingöl, N. (2016). In vitro micropropagation of critically endangered (cr) endemic Erodium somanum. Celal Bayar University Journal of Science, 12(1), 115-120. https://doi: 10.18466/cbujos.10321
  • [34] Sevindik, B., Tutuncu, M., Izgu, T., Tagipur, E. M., Curuk, P., Yilmaz, O., Kaynak, G. & Mendi, Y. Y. (2017). Micropropagation of Erodium olympicum endemic to Turkey. American Journal of Plant Biology, 2(3-1), 24-27. https://doi: 10.11648/j.ajpb.s.2017020501.14
  • [35] Cüce, M., Bekircan, T., Laghari, A.H., Sökmen, M., Sökmen, A., Önay Uçar, E. & Kılıç, A.O. (2017). Antioxidant phenolic constituents, antimicrobial and cytotoxic properties of Stachys annua L. from both natural resources and micropropagated plantlets. Indian Journal of Traditional Knowledge, 16(3), 407-416.
  • [36] Rabuma, T. (2015). In vitro propagation of geranium (Pelargonium graveolens L.) from nodal culture. Journal of Agriculture Science and Technology, 4(2), 23-34.
  • [37] Ružić, D., Vujović, T., Libiakova, G., Cerović, R. & Gajdošova, A. (2012). Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). Journal of Berry Research, 2, 97-103. https://doi: 10.3233/JBR-2012-0

Erodium hoefftianum (Geraniaceae)’un Doku Kültürü Yöntemleriyle Üretiminin Araştırılması

Year 2021, Volume 14, Issue 3, 418 - 426, 15.12.2021

Abstract

This current and novel approach was designed to occur the production method of Erodium hoefftianum via direct organogenesis. Lateral meristems obtained from seeds germinated in Murashige and Skoog (MS) basal medium were preferred as explants. Then, MS medium supplemented with individually 1.0/0.1 mg/L zeatin (ZEA)/indole-3- butyric acid (IBA), 1.0/0.1 mg/L kinetin (KIN)/IBA, 1.0/0.1 mg/L N6-[2-isopentenil]adenine (2iP)/IBA, 1.0/0.1 mg/L 6- Benzyladenine (6-BA)/IBA, 1.0/0.1 mg/L thidiazuron (TDZ)/IBA and without plant growth regulators (PGRs) were investigated for the effective shoot multiplication. 1.0/0.1 mg/L 6-BA/ IBA combination was found to be the most appropriate PGRs in all cases except shoot length. Zeatin/IBA combination was determined to be most ideal for shoot length with 37.28 mm. The rooting capability was also studied by using MS with IBA, indole-3- butyric acid (IAA), and 1-naphthaleneacetic acid, (NAA) (0.5 mg/L) or without auxin (control). The control group has the highest values in all cases with the rooting percentage at 100%. Well rooted and healty plantlets were transplanted into rich peat: perlite (2:1) substrates and subsequently acclimatized carefully under climate room conditions and transfer to the botanical garden conditions.

References

  • [1] Takhtajan, A. (1997). Diversity and classification of flowering plants. New York: Columbia University Press.
  • [2] Aldasoro, J. J., Navarro, C., Vargas, P., Sa´Ez, L. L. & Aedo, C. C. (2002). A new genus of Geraniaceae endemic to the Southwest of North America. Anales del Jardin Botanico de Madrid, 59, 209-216. https://doi: 10.3989/ajbm.2001.v
  • [3] Fiz, O., Vargas, P., Alarcon, M. L. & Aldasoro, J. J. (2006). Phylogenetic relationships and evolution in Erodium (Geraniaceae) based on trnL-trnF sequences. Systematic Botany, 31, 739-763. https://doi: 10.1600/036364406779695906
  • [4] Messing, S. & Byrne, R. (1998). Premission invasion of Erodium cicutarium in California. Journal of Biogeography, 25, 757-762. https://doi: 10.1046/j.1365-2699.1998.2540757.x
  • [5] Fiz-Palacios, O., Vargas, P., Vila, R., Papadopulos, A. S. & Aldasoro, J. J. (2010). The uneven phylogeny and biogeography of Erodium (Geraniaceae): radiations in the Mediterranean and recent recurrent intercontinental colonization. Annals of Botany, 106(6), 871-884. https://doi: 10.1093/aob/mcq184
  • [6] Davis, P.H. 1988. Flora of Turkey and East Egean Islands. Edinburgh, UK: Edinburgh University Press, 10, 105-106.
  • [7] Güner, A., Özhatay, N., Ekim, T. & Başer, K. H. C. (2000). Flora of Turkey and East Eagean Islands. Edinburgh, UK: Edinburgh University Press, 11, 74-75.
  • [8] Yıldırımlı, Ş. & Doğru-Koca, A. (2004). A new species from Turkey, Erodium aytacii Yıldırımlı & A. Doğru-Koca (Geraniaceae). Ot Sistematik Botanik Dergisi, 11, 1-6.
  • [9] Radhia, A., Hanen, N., Abdelkarim, B. A. & Mohamed, N. (2018). Phytochemical screening, antioxidant and antimicrobial activities of Erodium glaucophyllum (L.) L’Hérit. Journal of Biomedical Sciencies, 7(4). https://doi: 10.4172/2254-609X.100092
  • [10] Bilić, V. L., Gašić, U., Milojković‐Opsenica, D., Nemet, I., Rončević, S., Kosalec, I. & Rodriguez, J. V. (2020). First Extensive Polyphenolic Profile of Erodium cicutarium with Novel Insights to Elemental Composition and Antioxidant Activity. Chemistry & Biodiversity, 17(9), e2000280. https://doi.org/10.1002/cbdv.202000280
  • [11] Yıldırım, H., Bekircan, Ç. & Bektaş, E. (2019). Antifeedant effects of essential oil of Mentha longifolia subsp. longifolia L. HUDSON (Lamiaceae) on Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae). Biological Diversity and Conservation, 12(2), 103-108. https://doi: 10.5505/biodicon.2019.27928
  • [12] Edenharder, R. & Tang, X. (1997). Inhibition of the mutagenicity of 2-nitrofluorene, 3-nitrofluoranthene and 1-nitropyrene by flavonoids, coumarins, quinones and other phenolic compounds. Food and Chemical Toxicology, 35(3-4), 357-372. https://doi: 10.1016/S0278-6915(97)00125-7
  • [13] Narayana, K. R., Reddy, M. S., Chaluvadi, M. R. & Krishna, D. R. (2001). Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian journal of pharmacology, 33(1), 2-16.
  • [14] Seyoum, A., Asres, K. & El-Fiky, F. K. (2006). Structure–radical scavenging activity relationships of flavonoids. Phytochemistry, 67(18), 2058-2070.
  • [15] Middleton, E., Kandaswami, C. & Theoharides, T. C. (2000). The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological reviews, 52(4), 673-751.
  • [16] Kekilli, E. B., Orhan, I. E., Deniz, F. S. S., Eren, G., Emerce, E., Kahraman, A., & Aysal, I. A. (2021). Erodium birandianum Ilarslan & Yurdak. shows anti-gout effect through xanthine oxidase inhibition: Combination of in vitro and in silico techniques and profiling of main components by LC-Q-ToF-MS. Phytochemistry Letters, 43, 80-87. https://doi.org/10.1016/j.phytol.2021.03.010
  • [17] Molares, S. & Ladio, A. (2009). Ethnobotanical review of the Mapuche medicinal flora: use patterns on a regional scale. Journal of ethnopharmacology, 122(2), 251-260. https://doi: 10.1016/j.jep.2009.01.003
  • [18] Munekata, P. E., Alcántara, C., Collado, M. C., Garcia-Perez, J. V., Saraiva, J. A., Lopes, R. P., Barba, F. J., Silva, L. P., Sant'Ana, A. S., Fierro, E. M. & Lorenzo, J. M. (2019). Ethnopharmacology, phytochemistry and biological activity of Erodium species: A review. Food research international, 126, 108659. https://doi: 10.1016/j.foodres.2019.108659
  • [19] Mosaddegh, M., Naghibi, F., Moazzeni, H., Pirani, A. & Esmaeili, S. (2012). Ethnobotanical survey of herbal remedies traditionally used in Kohghiluyeh va Boyer Ahmad province of Iran. Journal of ethnopharmacology, 141(1), 80-95. https://doi: 10.1016/j.jep.2012.02.004
  • [20] Rehman, K., Khan, M. A., Ullah, Z. & Chaudhary, H. J. (2015). An ethno botanical perspective of traditional medicinal plants from the Khattak tribe of Chonthra Karak, Pakistan. Journal of ethnopharmacology, 165, 251-259. https://doi: 10.1016/j.jep.2015.02.035
  • [21] Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. & Adıgüzel, N. (2000). Türkiye Bitkileri Kırmızı Kitabı. Ankara: Türkiye Tabiatını Koruma Derneği.
  • [22] Ostrolucká, M. G., Libiakov,á G., Ondrušková, E. & Gajdošová, A. (2004). In vitro propagation of Vaccinium species. Acta Universitatis Latviensis, 676, 207-212.
  • [23] Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi: 10.1111/j.1399-3054.1962.tb08052.x
  • [24] Lambardi, M., Sharma, K. K. & Thorpe, T. A. (1993). Optimization of in vitro bud induction and plantlet formation from mature embryos of Aleppo pine (Pinus halepensis Mill.). In Vitro Cellular & Developmental Biology – Plant, 29, 189-199.
  • [25] Koc, M., Hamzaoğlu, E. & Özhatay, F. N. (2014). Arenaria microcalyx (Caryophyllaceae), a new species from Turkey. Turkish Journal of Botany, 38(4), 653-657. https://doi: 10.3906/bot-1311-27
  • [26] Corral, P., Mallon, R., Oubina-Rodriguez, J. & Gonzalez, M. L. (2011). Multiple shoot ınduction and plant regeneration of the endengered species Crepis novoana. Plant Cell Tissue and Organ Culture, 105, 211-217. https://doi: 10.1007/s11240-010-9854-x
  • [27] Lemay, M., De Vriendt, L., Pellerin, S. & Poulin, M. (2015). Ex situ germination as a method for seed viability assessment in a peatland orchid, Plantanthera blephariglottis. American Journal of Botany, 102, 390-395. https://doi: 10.3732/ajb.1400441
  • [28] Zhang, T., Cao, Z. Y. & Wang, X. Y. (2005). Induction of somatic embryogenesis and plant regeneration from cotyledon and hypocotyl explants of Eruca sativa Mill. In Vitro Cellular & Developmental Biology-Plant, 41(5), 655-657. https://doi: 10.1079/IVP2005653
  • [29] Cüce, M. & Sökmen, A. (2017). In vitro production protocol of Vaccinium uliginosum L. (bog bilberry) growing in the Turkish flora. Turkish Journal of Agriculture and Forestry, 41, 294-304. https://doi: 10.3906/tar-1704-19
  • [30] Bekircan, T., Yaşar, A., Yıldırım, S., Sökmen, M. & Sökmen, A. (2018). Effect of cytokinins on in vitro multiplication, volatiles composition and rosmarinic acid content of Thymus leucotrichus Hal. shoots. 3 Biotech, 8(3), 180. https://doi: 10.1007/s13205-018-1206-2
  • [31] Cüce, M., Bekircan, T., Laghari, A. H., Sökmen, M., Sökmen, A., Önay Uçar, E. & Kılıç, A. O. (2019). Phenolic profiles, antimicrobial and cytotoxic properties of both micropropagated and naturally growing plantlets of Calamintha sylvatica subsp. sylvatica Bromf. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(4), 1145-1152. https://doi: 10.15835/nbha47411474
  • [32] Akın, B., Kocaçalışkan, İ. & Güleryüz, G. (2014). Micropropagation of Erodium sibthorpianum subsp. sibthorpianum, an endemic threatened species of Uludağ Mountain (Bursa-Turkey). Turkish Journal of Botany, 38(1), 148-155. https://doi: 10.3906/bot-1304-24
  • [33] Çetin, B., Eren, H., Oskay, D. & Bingöl, N. (2016). In vitro micropropagation of critically endangered (cr) endemic Erodium somanum. Celal Bayar University Journal of Science, 12(1), 115-120. https://doi: 10.18466/cbujos.10321
  • [34] Sevindik, B., Tutuncu, M., Izgu, T., Tagipur, E. M., Curuk, P., Yilmaz, O., Kaynak, G. & Mendi, Y. Y. (2017). Micropropagation of Erodium olympicum endemic to Turkey. American Journal of Plant Biology, 2(3-1), 24-27. https://doi: 10.11648/j.ajpb.s.2017020501.14
  • [35] Cüce, M., Bekircan, T., Laghari, A.H., Sökmen, M., Sökmen, A., Önay Uçar, E. & Kılıç, A.O. (2017). Antioxidant phenolic constituents, antimicrobial and cytotoxic properties of Stachys annua L. from both natural resources and micropropagated plantlets. Indian Journal of Traditional Knowledge, 16(3), 407-416.
  • [36] Rabuma, T. (2015). In vitro propagation of geranium (Pelargonium graveolens L.) from nodal culture. Journal of Agriculture Science and Technology, 4(2), 23-34.
  • [37] Ružić, D., Vujović, T., Libiakova, G., Cerović, R. & Gajdošova, A. (2012). Micropropagation in vitro of highbush blueberry (Vaccinium corymbosum L.). Journal of Berry Research, 2, 97-103. https://doi: 10.3233/JBR-2012-0

Details

Primary Language English
Subjects Biodiversity Conservation
Journal Section Research Article
Authors

Mustafa CÜCE (Primary Author)
GİRESUN ÜNİVERSİTESİ
0000-0001-7406-171X
Türkiye

Publication Date December 15, 2021
Application Date August 11, 2021
Acceptance Date December 4, 2021
Published in Issue Year 2021, Volume 14, Issue 3

Cite

APA Cüce, M. (2021). Erodium hoefftianum (Geraniaceae)’un Doku Kültürü Yöntemleriyle Üretiminin Araştırılması . Biyolojik Çeşitlilik ve Koruma , 14 (3) , 418-426 . Retrieved from https://dergipark.org.tr/en/pub/biodicon/issue/66302/981474

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❖ Please visit ” http:// www.biodicon.com“ for instructions about articles and all of the details about journal


❖  Correspondance Adres: Prof. Ersin YÜCEL, P.K. 86, İki Eylül PTT Merkez, 26000 Eskişehir / Turkey
E-posta / E-mail: biodicon@gmail.com;
Web Address: http://www.biodicon.com; https://dergipark.org.tr/en/pub/biodicon
❖ Biological Diversity and Conservation/ Biyolojik Çeşitlilik ve Koruma
❖ ISSN 1308-5301 Print; ISSN 1308-8084 Online
❖ Start Date Published 2008
© Copyright by Biological Diversity and Conservation/Biyolojik Çeşitlilik ve Koruma-Available online at www.biodicon.com/All rights reserved
Publisher : Ersin YÜCEL
❖ This journal is published three numbers in a year. Printed in Eskişehir/Turkey.
❖ All sorts of responsibilities of the articles published in this journal are belonging to the authors
Editör / Editor-In-Chief : Prof.Dr. Ersin YÜCEL, https://orcid.org/0000-0001-8274-7578