Research Article
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Topraktan izole edilen aktinomisetlerin lipaz aktivitelerinin belirlenmesi

Year 2021, Volume 14, Issue 3, 487 - 492, 15.12.2021
https://doi.org/10.46309/biodicon.2021.988928

Abstract

Bu çalışmada farklı toprak örneklerinden izole edilmiş 156 aktinomiset izolatı kullanılmıştır. İzolatlar uygun besiyerine ekilmiş ve 28°C’de 7-10 gün inkübe edilmiştir. İzolatların kültürel ortamda lipolitik aktivitelerinin incelenmesi amacıyla, hazırlanan lipaz besiyerinde izolatların oluşturdukları şeffaf zon cetvel ile ölçülmüştür. Bunlardan 53 tanesi (%34) 10 mm ve daha az, 99 tanesi (%63) 20 mm ve daha az, 4 tanesi (%3) ise 20 mm’den daha fazla şeffaf zon oluşturmuştur. Tribütirinli besiyerinde en fazla şeffaf zon oluşturan 47 numaralı (21,5 mm) izolatın 16S rRNA gen bölgesine göre yapılan dizi analizi sonucunda GenBank numarası EU283368.1 olan ‘uncultured actinobacterium clone AS42 16S ribosomal RNA gene’ e %97 oranında benzerlik gösterdiği görülmüştür. İzolatın elde edilen 16S rRNA dizi bilgisi GenBank’a KY172825 erişim numarası ile kaydedilmiştir.

References

  • [1] Ventura, M., Canchaya, C., Tauch, A., Chandra, G., Fitzgerald, G. F., Chater, K. F., van Sinderen, D. (2007). Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum. Microbiology and Molecular Biology Reviews, 71(3), 495–548. http://doi.org/10.1128/MMBR.00005-07.
  • [2] Boyd, R.F. (1988). General microbiology. St. Louis, USA: Times Mirror/Mosby College Publishing.
  • [3] Gupta, R., Gupta, N., Rathi, P. (2004). Bacterial lipases: An overview of production, purification and biochemical properties. Applied Microbiology and Biotechnology, 64(6), 763–781. http://doi.org/10.1007/s00253-004-1568-8.
  • [4] Treichel, H., de Oliveira, D., Mazutti, M. A., Di Luccio, M., Oliveira, J. V. (2010). A review on microbial lipases production. Food and Bioprocess Technology, 3(2), 182–196. http://doi.org/10.1007/s11947-009-0202-2.
  • [5] Sharma, A. D., Singh, J. (2005). A nonenzymatic method to isolate genomic DNA from bacteria and actinomycete. Analytical Biochemistry, 337(2), 354–356. http://doi.org/10.1016/j.ab.2004.11.029.
  • [6] Ramesh, S., Mathivanan, N. (2009). Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World Journal of Microbiology and Biotechnology, 25(12), 2103–2111. http://doi.org/10.1007/s11274-009-0113-4.
  • [7] Shah, A. M., Shakeel-u-Rehman, Hussain, A., Mushtaq, S., Rather, M. A., Shah, A., Ahmad, Z., Khan I. A., Hassan, Q. P. (2017). Antimicrobial investigation of selected soil actinomycetes isolated from unexplored regions of Kashmir Himalayas, India. Microbial Pathogenesis, 110, 93–99. http://doi.org/10.1016/j.micpath.2017.06.017.
  • [8] Hasan, N. A., Nawahwi, M. Z., Yahya, N., Othman, N. A. (2018). Identification and optimization of lipase producing bacteria from palm oil contaminated waste. Journal of Fundamental and Applied Sciences. 10(2S), 300-310. http://dx.doi.org/10.4314/jfas.v10i2s.23.
  • [9] Praveen Kumar, P., Sagaya Jansi, R., Saravana Kumar, P., Nimal Christhudas, I. V. S., Preetam Raj, J. P., Vijayakumar, A., Agastian, P., Ignacimuthu, S. (2017). Optimization of biosynthesis parameters, partial purification and characterization of extracellular lipase from soil derived Streptomyces sp. Loyola Lipase-1. Biocatalysis and Agricultural Biotechnology, 12(10), 241–247. http://doi.org/10.1016/j.bcab.2017.10.011.
  • [10] Veerapagu, M., Narayanan, A. S., Ponmurugan, K., Jeya, K. R. (2013). Screening Selection Identification Production and Optimization of Bacterial Lipase From Oil Spilled Soil. Asian Journal of Pharmaceutical and Clinical Research. 6(3), 62-67.

Determination of lipase activities of actinomycetes isolates from soil

Year 2021, Volume 14, Issue 3, 487 - 492, 15.12.2021
https://doi.org/10.46309/biodicon.2021.988928

Abstract

In this study 156 actinomycetes isolates which were isolated from different soil samples were used. Isolates were inoculated proper medium and incubated at 28°C for 7-10 days. In order to investigate the lipolytic activities of the isolates in the culture medium, the transparent zone formed by isolates in the prepared lipase medium was measured with a ruler. 53 of them (34 %) 10 mm and less, 99 of them (63 %) 20 mm and less, 4 of them (3 %) more than 20 mm transparent zone produced. The isolate which showed the highest lipase activity molecular identification has been made which based on 16S rRNA gene sequence. The 16S rRNA sequence information obtained from the isolate was recorded to GenBank with the access number KY172825. As a result of the study has been made on the NCBI database by using BLAST program, it was observed that, isolate showed 97% similarity ‘uncultured actinobacterium clone AS42 16S ribosomal RNA gene’ which’s access number on the database is EU283368.1.

References

  • [1] Ventura, M., Canchaya, C., Tauch, A., Chandra, G., Fitzgerald, G. F., Chater, K. F., van Sinderen, D. (2007). Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum. Microbiology and Molecular Biology Reviews, 71(3), 495–548. http://doi.org/10.1128/MMBR.00005-07.
  • [2] Boyd, R.F. (1988). General microbiology. St. Louis, USA: Times Mirror/Mosby College Publishing.
  • [3] Gupta, R., Gupta, N., Rathi, P. (2004). Bacterial lipases: An overview of production, purification and biochemical properties. Applied Microbiology and Biotechnology, 64(6), 763–781. http://doi.org/10.1007/s00253-004-1568-8.
  • [4] Treichel, H., de Oliveira, D., Mazutti, M. A., Di Luccio, M., Oliveira, J. V. (2010). A review on microbial lipases production. Food and Bioprocess Technology, 3(2), 182–196. http://doi.org/10.1007/s11947-009-0202-2.
  • [5] Sharma, A. D., Singh, J. (2005). A nonenzymatic method to isolate genomic DNA from bacteria and actinomycete. Analytical Biochemistry, 337(2), 354–356. http://doi.org/10.1016/j.ab.2004.11.029.
  • [6] Ramesh, S., Mathivanan, N. (2009). Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World Journal of Microbiology and Biotechnology, 25(12), 2103–2111. http://doi.org/10.1007/s11274-009-0113-4.
  • [7] Shah, A. M., Shakeel-u-Rehman, Hussain, A., Mushtaq, S., Rather, M. A., Shah, A., Ahmad, Z., Khan I. A., Hassan, Q. P. (2017). Antimicrobial investigation of selected soil actinomycetes isolated from unexplored regions of Kashmir Himalayas, India. Microbial Pathogenesis, 110, 93–99. http://doi.org/10.1016/j.micpath.2017.06.017.
  • [8] Hasan, N. A., Nawahwi, M. Z., Yahya, N., Othman, N. A. (2018). Identification and optimization of lipase producing bacteria from palm oil contaminated waste. Journal of Fundamental and Applied Sciences. 10(2S), 300-310. http://dx.doi.org/10.4314/jfas.v10i2s.23.
  • [9] Praveen Kumar, P., Sagaya Jansi, R., Saravana Kumar, P., Nimal Christhudas, I. V. S., Preetam Raj, J. P., Vijayakumar, A., Agastian, P., Ignacimuthu, S. (2017). Optimization of biosynthesis parameters, partial purification and characterization of extracellular lipase from soil derived Streptomyces sp. Loyola Lipase-1. Biocatalysis and Agricultural Biotechnology, 12(10), 241–247. http://doi.org/10.1016/j.bcab.2017.10.011.
  • [10] Veerapagu, M., Narayanan, A. S., Ponmurugan, K., Jeya, K. R. (2013). Screening Selection Identification Production and Optimization of Bacterial Lipase From Oil Spilled Soil. Asian Journal of Pharmaceutical and Clinical Research. 6(3), 62-67.

Details

Primary Language Turkish
Subjects Biodiversity Conservation, Biochemistry and Molecular Biology
Journal Section Research Article
Authors

Gizem ARIK (Primary Author)
ANKARA MEDİPOL ÜNİVERSİTESİ
0000-0002-3673-3543
Türkiye


Nalan YILMAZ SARIÖZLÜ
ESKİŞEHİR TEKNİK ÜNİVERSİTESİ
0000-0003-2485-2016
Türkiye

Supporting Institution Eskişehir Teknik Üniversitesi
Project Number 1005F114
Publication Date December 15, 2021
Application Date August 31, 2021
Acceptance Date November 12, 2021
Published in Issue Year 2021, Volume 14, Issue 3

Cite

APA Arık, G. & Yılmaz Sarıözlü, N. (2021). Topraktan izole edilen aktinomisetlerin lipaz aktivitelerinin belirlenmesi . Biyolojik Çeşitlilik ve Koruma , 14 (3) , 487-492 . DOI: 10.46309/biodicon.2021.988928

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❖ Biological Diversity and Conservation/ Biyolojik Çeşitlilik ve Koruma
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