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Analysis of cytochrome c oxidase subunit I gene of Canis lupus in Turkey

Year 2025, Volume: 18 Issue: 1, 58 - 63
https://doi.org/10.46309/biodicon.2025.1456557

Abstract

This study is based on the analysis of the cytochrome c oxidase subunit I gene of 20 grey wolf samples obtained from different regions of Turkey between 2014-2018. The results from this study were compared with sequences from Canis lupus records from other localities available in GenBank. This comparison showed that Canis lupus in Turkey originates from the same genetic source as the Canis lupus sequences in GenBank with a very high degree of similarity in sequencing (97-100%).

References

  • [1] Avise, J.C. (2004). Molecular Markers, Natural History, and Evolution. 2nd Ed. Sunderland, MA; Oxford: Oxford University Press. http://dx.doi.org/10.1007/978-1-4615-2381-9
  • [2] Fonseca, R.R., Johnson, W.E., O'Brien, S.J., Ramos, M.J., Antunes, A. (2008). The adaptive evolution of the mammalian mitochondrial genome. BMC Genomics. 9 (119):1-22. https://doi.org/ 10.1186/1471-2164-9-119
  • [3] Hebert, P.D.N., Ratnasingham, S., Dewaard, J.R., 2003a. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings Of The Royal Society B: Biological Sciences. 270:96-99. [4] Hebert, P.D.N., Cywinska A, Ball SL, deWaard JR (2003b). Biological identifications through DNA barcodes. Proceedings Of The Royal Society of London Series B: Biological Sciences. 270: 313-321. https://doi.org/ 10.1098/rspb.2002.2218
  • [5] İbiş, O., Aksöyek, E., Özcan, S., Keten, A., Yorulmaz, T., & Tez, C. (2016). Genetic analysis of the Turkish gray wolf (Canis lupus) based on partial mitochondrial DNA sequences. Vertabrate Zoology, 66 (3), 427-435.
  • [6] Koblmuller, S., Vila, C., Lorente-Galdos, B., Dabad, M., Ramirez, O. et al. (2016). Whole mitochondrial genomes illuminate ancient intercontinental dispersals of grey wolves (Canis lupus). Journal of Biogeography, 43 (9): 1728-1738. https://doi.org/ 10.1111/jbi.12765
  • [7] Li, Y., Zhao, X., Pan, Z., Xie, Z., Liu, H. et al. (2011). The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding. Animal, 5 (12): 1868-1873. https://doi.org/ 10.1017/S1751731111001042
  • [8] Ölmez, B., & Demirbaş, Y. (2024). Türkiye’deki Canis lupus (Gri Kurt)’un Dağılımı ve Genetik Yapısı. International Journal of Engineering Research & Development (IJERAD), 16(2). https://doi.org/10.29137/umagd.1416917
  • [9] Thalmann, O., Shapiro, B., Cuıv, P., Schuenemann, V.J., Sawyer, S.K. (2013). Complete Mitochondrial Genomes of Ancient Canids Suggest a European Origin of Domestic Dogs. Science, 342 (6160): 871-874. https://doi.org/10.1126/science.1243650
  • [10] Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G. (1998). The CLUSTAL_X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools. Nucleic Acids Research, 25 (24): 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  • [11] Vilà, C., Amorim, I. R., Leonard, J. A., Posada, D., Castroviejo, J., Petrucci‐Fonseca, F., ... & Wayne, R. K. (1999). Mitochondrial DNA phylogeography and population history of the grey wolf Canis lupus. Molecular Ecology, 8 (12), 2089-2103. https://doi: 10.1046/j.1365-294x.1999.00825.x
  • [12] Zahn, L.M. (2016). Effects of mtDNA and nuclear genetic variation. Science, 353 (6305): 1246-1247. https://doi.org/ 10.1126/science.353.6305.1246-c.

Türkiye'deki Canis lupus'un sitokrom c oksidaz subunit I geninin analizi

Year 2025, Volume: 18 Issue: 1, 58 - 63
https://doi.org/10.46309/biodicon.2025.1456557

Abstract

Bu çalışma, 2014-2018 yılları arasında Türkiye'nin farklı bölgelerinden elde edilen 20 adet gri kurt örneğinin sitokrom c sksidaz subunit I geninin analizine dayanmaktadır. Bu çalışmadan elde edilen sonuçlar, GenBank'ta bulunan diğer bölgelerdeki Canis lupus kayıtlarından elde edilen dizilerle karşılaştırıldı. Bu karşılaştırma, Türkiye'deki Canis lupus'un Gen Bankası'ndaki Canis lupus dizileriyle aynı genetik kaynaktan geldiği ve dizilim açısından çok yüksek derecede benzerlik gösterdiğin (%97-100) kaydedildi.

References

  • [1] Avise, J.C. (2004). Molecular Markers, Natural History, and Evolution. 2nd Ed. Sunderland, MA; Oxford: Oxford University Press. http://dx.doi.org/10.1007/978-1-4615-2381-9
  • [2] Fonseca, R.R., Johnson, W.E., O'Brien, S.J., Ramos, M.J., Antunes, A. (2008). The adaptive evolution of the mammalian mitochondrial genome. BMC Genomics. 9 (119):1-22. https://doi.org/ 10.1186/1471-2164-9-119
  • [3] Hebert, P.D.N., Ratnasingham, S., Dewaard, J.R., 2003a. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings Of The Royal Society B: Biological Sciences. 270:96-99. [4] Hebert, P.D.N., Cywinska A, Ball SL, deWaard JR (2003b). Biological identifications through DNA barcodes. Proceedings Of The Royal Society of London Series B: Biological Sciences. 270: 313-321. https://doi.org/ 10.1098/rspb.2002.2218
  • [5] İbiş, O., Aksöyek, E., Özcan, S., Keten, A., Yorulmaz, T., & Tez, C. (2016). Genetic analysis of the Turkish gray wolf (Canis lupus) based on partial mitochondrial DNA sequences. Vertabrate Zoology, 66 (3), 427-435.
  • [6] Koblmuller, S., Vila, C., Lorente-Galdos, B., Dabad, M., Ramirez, O. et al. (2016). Whole mitochondrial genomes illuminate ancient intercontinental dispersals of grey wolves (Canis lupus). Journal of Biogeography, 43 (9): 1728-1738. https://doi.org/ 10.1111/jbi.12765
  • [7] Li, Y., Zhao, X., Pan, Z., Xie, Z., Liu, H. et al. (2011). The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding. Animal, 5 (12): 1868-1873. https://doi.org/ 10.1017/S1751731111001042
  • [8] Ölmez, B., & Demirbaş, Y. (2024). Türkiye’deki Canis lupus (Gri Kurt)’un Dağılımı ve Genetik Yapısı. International Journal of Engineering Research & Development (IJERAD), 16(2). https://doi.org/10.29137/umagd.1416917
  • [9] Thalmann, O., Shapiro, B., Cuıv, P., Schuenemann, V.J., Sawyer, S.K. (2013). Complete Mitochondrial Genomes of Ancient Canids Suggest a European Origin of Domestic Dogs. Science, 342 (6160): 871-874. https://doi.org/10.1126/science.1243650
  • [10] Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G. (1998). The CLUSTAL_X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools. Nucleic Acids Research, 25 (24): 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  • [11] Vilà, C., Amorim, I. R., Leonard, J. A., Posada, D., Castroviejo, J., Petrucci‐Fonseca, F., ... & Wayne, R. K. (1999). Mitochondrial DNA phylogeography and population history of the grey wolf Canis lupus. Molecular Ecology, 8 (12), 2089-2103. https://doi: 10.1046/j.1365-294x.1999.00825.x
  • [12] Zahn, L.M. (2016). Effects of mtDNA and nuclear genetic variation. Science, 353 (6305): 1246-1247. https://doi.org/ 10.1126/science.353.6305.1246-c.
There are 11 citations in total.

Details

Primary Language English
Subjects Animal Systematics and Taxonomy
Journal Section Research Articles
Authors

Elif Yildiz Ay 0000-0003-4549-7789

İrfan Albayrak 0000-0002-9340-5749

Early Pub Date February 24, 2025
Publication Date
Submission Date March 25, 2024
Acceptance Date October 28, 2024
Published in Issue Year 2025 Volume: 18 Issue: 1

Cite

APA Yildiz Ay, E., & Albayrak, İ. (2025). Analysis of cytochrome c oxidase subunit I gene of Canis lupus in Turkey. Biological Diversity and Conservation, 18(1), 58-63. https://doi.org/10.46309/biodicon.2025.1456557

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