Background: This study aimed to determine the antiproliferative and cytotoxic activities of Papaver somniferum seed oil on human breast cancer (MDA-MB-231) cell lines under in vitro conditions.
Methods: Poppy seed oil extracted by cold press method were subjected to compositional analysis using GC-MS. In the study, control group, DMSO (0.1%) applied group and 5 different concentrations of Poppy seed oil applied group three main treatment groups were created. Cell viability was investigated by the trypan blue method. Some pharmacokinetic properties and toxicity profiles of the identified molecules were predicted using a Swiss ADME online server and ProTox-II webserver.
Results: The effect of Poppy seed oil on breast cancer cells increased relatively starting from the lowest concentration (87 µg/ml), and it was determined that the effect became more pronounced after a concentration of 350 µg/ml. Except Hexadecanoic acid and (E)-9- Octadecanoic acid, all other compounds were predicted to be non-hepatotoxic, non-carcinogenic, non-cytotoxic, non-immunotoxic and non-mutagenic. All compounds investigated are orally absorbable, suitable for dermal trans-distribution, have high gastrointestinal absorption, however, Hexadecanoic acid is permeable to the blood-brain barrier (BBB), while the other 4 compounds are not. The bioavailability values of all compounds investigated can be considered to be very well absorbed by the body.
Conclusion: In this study, it was determined that P. somniferum seed oil has antiproliferative and cytotoxic activity on breast cancer cells in vitro. P. somniforum seed oil may be a natural anticancer agent to treat breast cancer, but more studies are needed on this subject.
Conflicts of interest: The authors have no conflicts of interest to declare. Financial declaration: The authors have no relevant financial or non-financial interests to disclose. Authors' contributions: All authors contributed to the study conception and design. All authors read and approved the final manuscript.
Amaç: Bu çalışmada, Papaver somniferum tohum yağının insan meme kanseri (MDA-MB-231) hücre hatları üzerindeki antiproliferatif ve sitotoksik aktivitelerinin in vitro koşullarda belirlenmesi amaçlanmıştır.
Metod: Soğuk pres yöntemiyle ekstrakte edilen haşhaş tohumu yağı, GC-MS kullanılarak kompozisyon analizine tabi tutulmuştur. Çalışmada, kontrol grubu, DMSO (%0,1) uygulanan grup ve 5 farklı konsantrasyonda haşhaş tohumu yağı uygulanan grup olmak üzere üç ana tedavi grubu oluşturulmuştur. Hücre canlılığı tripan mavisi yöntemi ile araştırılmıştır. Tanımlanan moleküllerin bazı farmakokinetik özellikleri ve toksisite profilleri, bir İsviçre ADME çevrimiçi sunucusu ve ProTox-II web sunucusu kullanılarak tahmin edilmiştir.
Bulgular: Haşhaş tohumu yağının meme kanseri hücreleri üzerindeki etkisi en düşük konsantrasyondan (87 µg/ml) başlayarak nispeten artmış ve etkinin 350 µg/ml konsantrasyonundan sonra daha belirgin hale geldiği belirlenmiştir. Heksadekanoik asit ve (E)-9- Oktadekanoik asit hariç, diğer tüm bileşiklerin hepatotoksik, kanserojen, sitotoksik, immünotoksik ve mutajenik olmadığı öngörülmüştür. İncelenen tüm bileşikler oral yoldan emilebilir, dermal trans-dağılım için uygundur ve yüksek gastrointestinal emilime sahiptir; ancak Heksadekanoik asit kan-beyin bariyerine (BBB) geçirgenken, diğer 4 bileşik geçirgen değildir. İncelenen tüm bileşiklerin biyoyararlanım değerlerinin vücut tarafından çok iyi emildiği düşünülebilir.
Sonuç: Bu çalışmada, P. somniferum tohum yağının in vitro meme kanseri hücreleri üzerinde antiproliferatif ve sitotoksik aktiviteye sahip olduğu belirlenmiştir. P. somniforum tohum yağının meme kanserini tedavi etmek için doğal bir antikanser ajanı olabileceği, ancak bu konuda daha fazla çalışmaya ihtiyaç duyulduğu belirtilmiştir.
Conflicts of interest: The authors have no conflicts of interest to declare. Funding: No funding received. Ethical statement: This study does not require ethical approval. Financial declaration: The authors have no relevant financial or non-financial interests to disclose. Authors' contributions: All authors contributed to the study conception and design. All authors read and approved the final manuscript.
Primary Language | English |
---|---|
Subjects | Biochemistry and Cell Biology (Other), Medical Education |
Journal Section | Research Articles |
Authors | |
Early Pub Date | October 2, 2025 |
Publication Date | October 6, 2025 |
Submission Date | September 19, 2025 |
Acceptance Date | September 30, 2025 |
Published in Issue | Year 2025 Volume: 18 Issue: 3 |
❖ Indexes
{Additional Web of Science Indexes: Zoological Record] Clavariate Analytic, Medical Reads (RRS), CrossRef;10.46309/biodicon.
❖ Libraries
Aberystwyth University; All libraries; Bath University; Birmingham University; Cardiff University; City University London; CONSER (Not UK Holdings); Edinburgh University; Essex University; Eskişehir Teknik Üniversitesi Library, Exeter University; EZB Electronic Journals Library; Feng Chia University Library; GAZİ Gazi University Library; Glasgow University; HEC-National Digital Library; Hull University; Imperial College London; Kaohsinug Medical University Library; ANKOS; Anadolu University Library; Lancaster University; Libros PDF; Liverpool University; London Metropolitan University; London School of Economics and Political Science; Manchester University; National Cheng Kung University Library; National ILAN University Library; Nottingham University; Open University; Oxford University; Queen Mary,University of London;Robert Gordon University; Royal Botanic Gardens, Kew; Sheffield Hallam University; Sheffield University; Shih Hsin University Library; Smithsonian Institution Libraries; Southampton University; Stirling University; Strathclyde University; Sussex University; The National Agricultural Library (NAL); The Ohio Library and Information NetWork; Trinity College Dublin; University of Washington Libraries; Vaughan Memorial Library; York University.
❖ Articles published in the journal “Biological Diversity and Conservation” are freely accessible. No article processing charge (APC) is charged.
❖ Additional Web of Science Indexes: Zoological Record, Clavariate Analytic
❖ This journal is a member of CrossRef;10.46309/biodicon.
❖ For published articles and full details about the journal, please visit http://www.biodicon.com; https://dergipark.org.tr/tr/yayin/biodicon.
❖ Correspondence Address:: Prof. Ersin YÜCEL, Sazova Mahallesi, Ziraat Caddesi, No.277 F Blok, 26005 Tepebaşı-Eskişehir/Türkiye
❖ E-mail: biodicon@gmail.com;
❖ Web Address: http://www.biodicon.com; https://dergipark.org.tr/tr/pub/biodicon
❖ Biological Diversity and Conservation
❖ ISSN 1308-5301 Print; ISSN 1308-8084 Online
❖ Publication Start Date 2008
❖ © Copyright by Biological Diversity and Conservation/Biyolojik Çeşitlilik ve Koruma; Available online at www.biodicon.com. All rights reserved.
. ❖ Publisher: ERSİN YÜCEL (https://www.ersinyucel.com.tr)
❖ This Journal is published three times a year. It is printed in Eskişehir, Türkiye.
❖ The authors are solely responsible for the articles published in this Journal .
❖ Editör : Prof.Dr. Ersin YÜCEL, https://orcid.org/0000-0001-8274-7578