Potential for the Use of the Entomopathogenic Fungus Beauveria bassiana in the Biological Control of Cryptolestes ferrugineus and Acanthoscelides obtectus

Year 2023, Volume: 6 Issue: 2, 88 - 96, 31.12.2023

Nasibe Tekiner , Elif Tozlu , Göksel Tozlu

https://doi.org/10.38059/biodiversity.1404980

Cited By: 2

Abstract

In this study, the effectiveness of different conidial suspensions of the entomopathogenic fungus Beauveria bassiana (Balsamo) on stored product pests Acanthoscelides obtectus (Bean Seed Beetle) (Coleoptera, Chrysomelidae) and Cryptolestes ferrugineus (Rusty Grain Beetle) (Coleoptera, Laemophloeidae) was aimed to be determined. For this purpose, suspensions of B. bassiana isolate at concentrations of 106, 107, and 108 conidia/ml were used, with sterile water as a negative control and a recommended Declare (EC 25 g/l) as a positive control. The application was set up in three replicates, with 10 adults in each replicate, and applied using the spraying method. To calculate the percentage of mortality, Petri dishes were regularly checked every 24 hours from the application, and the numbers of live and dead adults were recorded. According to the obtained data, it was determined that B. bassiana isolate was effective against C. ferrugineus at a rate of 80% and against A. obtectus at a rate of 56.67% after 168 hours. Among the applied suspensions, the most effective suspension was recorded at 108 conidia/ml. As a result, the entomopathogenic fungus B. bassiana (ET 10) isolate used as a biological control agent, is considered a promising biological control agent against the storage pests C. ferrugineus and A. obtectus.

Keywords

Acanthoscelides obtectus , Beauveria bassiana , Biological Control , Cryptolestes ferrugineus

Ethical Statement

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Supporting Institution

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Project Number

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Thanks

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Entomopatojen fungus Beauveria bassiana’nın Cyptolestes ferrugineus ve Acanthoscelides obtectus’un biyolojik mücadelesinde kullanılma potansiyeli

Abstract

Bu çalışmada, depolanmış ürün zararlılarından Acanthoscelides obtectus (Fasulye Tohum Böceği) (Coleoptera, Chrysomelidae) ve Cryptolestes ferrugineus (Küçük Kırma Biti) (Coleoptera, Laemophloeidae) üzerine entomopatojen fungus Beauveria bassiana (Balsamo)’nın farklı conidia süspansiyonlarının etkinliğinin belirlenmesi amaçlanmıştır. Bu amaçla, B. bassiana izolatının 106, 107 ve 108 conidia/ml süspansiyonları, negatif kontrol olarak steril su ve pozitif kontrol olarak ise tavsiye edilen Declare (EC 25 g/l Delthametrin) kullanılmıştır. Uygulama 3 tekerrürlü ve her tekerrürde 10’ar adet ergin olacak şekilde kurulmuş ve püskürtme yöntemi ile uygulama yapılmıştır. Yüzde ölüm oranlarını hesaplamak amacıyla uygulamadan itibaren düzenli olarak 24 saat aralıklarla Petrilerin kontrolleri yapılmış, canlı ve ölü ergin sayıları kaydedilmiştir. Elde edilen verilere göre; 168 saat sonunda B. bassiana (ET 10) izolatının C. ferrugineus’a karşı %80, A. obtectus’a karşı %56.67 oranlarında etkili olduğu belirlenmiştir. Uygulanan süspansiyonlar arasında en etkili süspansiyonun 108 conidia/ml olduğu kaydedilmiştir. Sonuç olarak biyolojik mücadele ajanı olarak kullanılan entomopatojen fungus B. bassiana (ET 10) izolatının depo zararlısı olan C. ferrugineus ve A. obtectus’a kaşı ümitvar bir biyolojik mücadele etmeni olarak kullanabileceği düşünülmektedir.

Keywords

Acanthoscelides obtectus , Beauveria bassiana , Biyolojik mücadele , Cryptolestes ferrugineus

Project Number

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