Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii (Amaryllidaceae)

Abstract

Natural products have gained the great interest due to their broad spectrum of biological activities. Galanthus krasnovii was dried at shade then extracted with hexane, dichloromethane, and ethyl acetate successively. After removing of solvent by reduced pressure, crude extracts of each solvent were yielded. Antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation and reducing power assays were carried out for each extract. Moreover, total phenolic content was determined. Total phenolic content of hexane-, dichloromethane-, and ethyl acetate extracts were found as 60.95 mg GAE/g extract, 71.90 GAE/g extract and 58.90 GAE/g extract respectively. Dichloromethane and ethyl acetate extract revealed the moderate antioxidant activity. 

Keywords

• Galanthus krasnovii,natural product,antioxidant

Galanthus krasnovii (Amaryllidaceae)'nin toplam fenolik içeriği ile antioksidan kapasitenin değerlendirilmesi

Abstract

Doğal ürünler geniş spektrumlu biyolojik aktivite göstermelerinden dolayı yoğun ilgi görmektedirler. Galanthus krasnovii gölgede kurutulduktan sonra sırasıyla hekzan, diklorometan ve etil asetat ile ekstrakte edildi. Düşük basınçta çözücü uzaklaştırıldıktan sonra her bir çözücünün ekstraktı elde edildi. Her bir ekstraktın, 1,1-difenil-2- pikralhidrazil(DPPH) radikal, 2,2'-azino-bis(3- etilbenzotiazolin-6-sülfirik asit) (ABTS) radikal katyon ve indirgeme gücü antioksidan çalışmaları gerçekleştirildi. Ayrıca, ekstraktların toplam fenolik içerikleri belirlendi. Hekzan-, diklorometan- ve etil asetat ekstraktlarının toplam fenolik içerikleri sırasıyla 60.95 mg GAE/g ekstrakt, 71.90 GAE/g ekstrakt ve 58.90 GAE/g ekstrakt olarak belirlendi. Diklorometan ve etil asetat ekstraktları orta derece aktivite gösterdi.

---

Keywords

• Galanthus krashnovii,doğal ürün,antioksidan

References

1. Aksit H, Çelik SM, Sen Ö, Erenler R, Demirtas I, Telci I, Elmastas M (2014). Complete isolation and characterization of polar portion of Mentha dumetorum water extract. Records of Natural Products 8:277-280.
2. Apak R, Güçlü K, Özyürek M, Karademir SE (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry 52:7970-7981.
3. Ay EB, Gül M, Açikgöz MA, Yarilgaç T, Kara ŞM (2018). Assessment of antioxidant activity of giant snowdrop (Galanthus elwesii Hook) extracts with their total phenol and flavonoid contents. Indian Journal of Pharmaceutical Education and Research 52:S128-S132.
4. Berkov S, Codina C, Bastida J (2012). The genus Galanthus: A source of bioactive compounds. In: Phytochemicals-A Global Perspective of Their Role in Nutrition and Health. IntechOpen, 235-254.
5. Blois MS (1958). Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200.
6. Bokov D, Samylina I (2016). Identification of Flavonoids in Homeopathic Mother Tinctures of Galanthus woronowii Losinsk. and Galanthus nivalis L. by Ultra-Performance Liquid Chromatography with Photodiode Arrays and Tandem Quadrupole Mass-Selective Detectors. Pharmaceutical Chemistry Journal 50:458-464.
7. Davis AP, Özhatay N (2001). Galanthus trojanus: a new species of Galanthus (Amaryllidaceae) from north‐western Turkey. Botanical Journal of the Linnean Society 137:409-412.
8. Demirtas I, Erenler R, Elmastas M, Goktasoglu A (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry 136:34-40.

9. Ellis J, Nathan P, Villemagne V, Mulligan R, Saunder T, Young K, Smith C, Welch J, Woodward M, Wesnes K (2009). Galantamine-induced improvements in cognitive function are not related to alterations in α 4 β 2 nicotinic receptors in early Alzheimer’s disease as measured in vivo by 2-[18 F] Fluoro-A-85380 PET. Psychopharmacology 202:79-91.
10. Elmastas M, Celik SM, Genc N, Aksit H, Erenler R, Gulcin İ (2018). Antioxidant activity of an Anatolian herbal tea—Origanum minutiflorum: isolation and characterization of its secondary metabolites. International Journal of Food Properties 21:374-384.
11. Elmastas M, Erenler R, Isnac B, Aksit H, Sen O, Genc N, Demirtas I (2016). Isolation and identification of a new neo-clerodane diterpenoid from Teucrium chamaedrys L. Natural Product Research 30:299-304.
12. Elmastas M, Ozturk L, Gokce I, Erenler R, Aboul-Enein HY (2004). Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Analytical Letters 37:1859-1869.
13. Elmastaş M, Telci İ, Akşit H, Erenler R (2015). Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices/Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması. Turkish Journal of Biochemistry 40:456-462.
14. Erenler R, Demirtas I, Karan T, Altun M, Gul F (2017). Inhibitory Effect of 6, 7-dimethoxy-5-hydroxyflavone on Human Cervix Carcinoma in Vitro. International Journal of Secondary Metabolite 4:512-516.
15. Erenler R, Meral B, Sen O, Elmastas M, Aydin A, Eminagaoglu O, Topcu G (2017). Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology 55:1646-1653.
16. Erenler R, Sen O, Aksit H, Demirtas I, Yaglioglu AS, Elmastas M, Telci İ (2016). Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. Journal of the Science of Food and Agriculture 96:822-836.
17. Erenler R, Sen O, Yaglioglu AS, Demirtas I (2016). Bioactivity-guided isolation of antiproliferative sesquiterpene lactones from Centaurea solstitialis L. ssp. solstitialis. Combinatorial Chemistry & High Throughput Screening 19:66-72.
18. Erenler R, Telci İ, Elmastaş M, Akşit H, Gül F, Tüfekçi AR, Demirtaş İ, Kayir Ö (2018). Quantification of flavonoids isolated from Mentha spicata in selected clones of Turkish mint landraces. Turkish Journal of Chemistry 42:1695-1705.
19. Erenler R, Telci I, Ulutas M, Demirtas I, Gul F, Elmastas M, Kayir O (2015). Chemical Constituents, Quantitative Analysis and Antioxidant Activities of Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt. Journal of Food Biochemistry 39:622-630.
20. Erenler R, Yilmaz S, Aksit H, Sen O, Genc N, Elmastas M, Demirtas I (2014). Antioxidant activities of chemical constituents isolated from Echinops orientalis Trauv. Records of Natural Products 8(1): 32-36.
21. Genç N, Yıldız İ, Karan T, Eminağaoğlu Ö, Erenler R (2019). Antioxidant activity and total phenolic contents of Galanthus woronowii (Amaryllidaceae). Turkish Journal of Biodiversity 2(1):1-5.
22. Guzel A, Aksit H, Elmastas M, Erenler R (2017). Bioassay-guided isolation and identification of antioxidant flavonoids from Cyclotrichium origanifolium (Labill.) Manden. and Scheng. Pharmacognosy Magazine 13(50):316-320.
23. Karan T, Altuner Z, Erenler R (2017). Growth and Metabolite Production of Chroococcus minutus Under Different Temperature and Light Conditions. Journal of New Results in Science 6:47-52.
24. Karan T, Erenler R (2017a). Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum. International Journal of Secondary Metabolite 4:16-19.
25. Karan T, Erenler R (2018). Fatty acid constituents and anticancer activity of Cladophora fracta (OF Müller ex Vahl) Kützing. Tropical Journal of Pharmaceutical Research 17:1977-1982.
26. Karan T, Erenler R (2017b). Screening of norharmane from seven cyanobacteria by high-performance liquid chromatography. Pharmacognosy Magazine 13:723-725.
27. Karan T, Yildiz I, Aydin A, Erenler R (2018). Inhibition of various cancer cells proliferation of bornyl acetate and essential oil from Inula graveolens (Linnaeus) Desf. Records of Natural Products 12:273-283.
28. Karimi E, Mehrabanjoubani P, Homayouni-Tabrizi M, Abdolzadeh A, Soltani M (2018). Phytochemical evaluation, antioxidant properties and antibacterial activity of Iranian medicinal herb Galanthus transcaucasicus Fomin. Journal of Food Measurement and Characterization 12:433-440.
29. Kintsurashvili L, Vachnadze V (2007). Plants of the Amaryllidaceae family grown and introduced in Georgia: a source of galanthamine. Pharmaceutical Chemistry Journal 41:492-494.
30. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26:1231-1237.
31. Sarikaya BB, Kaya GI, Onur MA, Bastida J, Somer NU (2013). Phytochemical investigation of Galanthus woronowii. Biochemical Systematics and Ecology 51:276-279.
32. Sasaki YF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Taniguchi K, Tsuda S (2002). The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 519:103-119.
33. Singleton VL, Slinkard K (1977). Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture 28:49-55.
34. Topçu G, Erenler R, Çakmak O, Johansson CB, Çelik C, Chai H-B, Pezzuto JM (1999). Diterpenes from the berries of Juniperus excelsa. Phytochemistry 50:1195-1199.
35. Yaglıoglu AS, Akdulum B, Erenler R, Demirtas I, Telci I, Tekin S (2013). Antiproliferative activity of pentadeca-(8E, 13Z) dien-11-yn-2-one and (E)-1, 8-pentadecadiene from Echinacea pallida (Nutt.) Nutt. roots. Medicinal Chemistry Research 22:2946-2953.
36. Yildiz I, Sen O, Erenler R, Demirtas I, Behcet L (2017). Bioactivity–guided isolation of flavonoids from Cynanchum acutum L. subsp. sibiricum (willd.) Rech. f. and investigation of their antiproliferative activity. Natural Product Research 31:2629-2633.
37. Zubov DA, Davis AP (2012). Galanthus panjutinii sp. nov.: A new name for an invalidly published species of Galanthus (Amaryllidaceae) from the northern Colchis area of Western Transcaucasia. Phytotaxa 50:55-63.