Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii (Amaryllidaceae)

Öz

Natural products have gained the great interest due to their broad spectrum of biological activities. Galanthus krasnovii was dried at shade then extracted with hexane, dichloromethane, and ethyl acetate successively. After removing of solvent by reduced pressure, crude extracts of each solvent were yielded. Antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation and reducing power assays were carried out for each extract. Moreover, total phenolic content was determined. Total phenolic content of hexane-, dichloromethane-, and ethyl acetate extracts were found as 60.95 mg GAE/g extract, 71.90 GAE/g extract and 58.90 GAE/g extract respectively. Dichloromethane and ethyl acetate extract revealed the moderate antioxidant activity. 

Anahtar Kelimeler

• Galanthus krasnovii,natural product,antioxidant

Galanthus krasnovii (Amaryllidaceae)'nin toplam fenolik içeriği ile antioksidan kapasitenin değerlendirilmesi

Öz

Doğal ürünler geniş spektrumlu biyolojik aktivite göstermelerinden dolayı yoğun ilgi görmektedirler. Galanthus krasnovii gölgede kurutulduktan sonra sırasıyla hekzan, diklorometan ve etil asetat ile ekstrakte edildi. Düşük basınçta çözücü uzaklaştırıldıktan sonra her bir çözücünün ekstraktı elde edildi. Her bir ekstraktın, 1,1-difenil-2- pikralhidrazil(DPPH) radikal, 2,2'-azino-bis(3- etilbenzotiazolin-6-sülfirik asit) (ABTS) radikal katyon ve indirgeme gücü antioksidan çalışmaları gerçekleştirildi. Ayrıca, ekstraktların toplam fenolik içerikleri belirlendi. Hekzan-, diklorometan- ve etil asetat ekstraktlarının toplam fenolik içerikleri sırasıyla 60.95 mg GAE/g ekstrakt, 71.90 GAE/g ekstrakt ve 58.90 GAE/g ekstrakt olarak belirlendi. Diklorometan ve etil asetat ekstraktları orta derece aktivite gösterdi.

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Anahtar Kelimeler

• Galanthus krashnovii,doğal ürün,antioksidan

Kaynakça

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