Research Article
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Year 2023, , 1 - 9, 30.06.2023
https://doi.org/10.38042/biotechstudies.1249244

Abstract

References

  • Arıcı, E. Ş. (2006). Somaklonal Varyasyondan Yararlanarak in vitro Seleksiyonla Buğday (Triticum aestivum L.)’da Başak Yanıklığına Dayanıklı Bitki Elde Edilmesi. [Yayımlanmamış Doktora Tezi]. Çukurova Üniversitesi.
  • Babaoğlu, M., Yorgancılar, M., & Akbudak, M. A. (2001). Doku Kültürü: Temel laboratuvar teknikleri, Bitki Biyoteknolojisi, Doku Kültürü ve Uygulamaları. Editörler: Babaoğlu, M. Gürel, E. Özcan, S. Konya, s.1-35.
  • Bado, S., Rafiri, M. A., El-Achouri, K., Sapey, E., Nielen, S., Ghanim, A. M. A., Forster, B.P., & Laimer, M. (2016). In vitro Methods for Mutation Induction in Potato (Solanum tuberosum L.). African Journal of Biotechnology 15(39): 2132-2145. https://doi.org/10.5897/AJB2016.15571
  • Ben-Hayyim, G., & Kochba, J. (1983). Aspects of Salt Tolerance in a NaCI-Selected Stable Cell Line of Citrus sinensis. Plant Physiol. 72: 685-690. https://doi.org/10.1104/pp.72.3.685
  • Bradley, P. M., El Fike, F., & Giles, K. L. (1984). Polyamines and Arginine Affect Somatic Embryogenesis of Daucus carota L. Plant Science Letters. 34: 397-401. https://doi.org/10.1016/S0304-4211(84)80021-8
  • Bükün, B., Gaines, T., Nissen, S. J., Westra, P., Shanner, D. L., Leach, J., Chisholm, S., Ward, S., Preston, C., Culpepper, S., Gray, T., Webster, T., Vencill, B., & Tranel, P. (2009). Amaranthus palmeri L.’nin Glyphosata Dayanıklılık Mekanizması. Türkiye III. Bitki Koruma Kongresi, 15-18 Temmuz, Van.
  • Dodds, J. H., & Roberts, L.W. (1982). Experiments in Plant Tissue Culture sec. Edi. Cambridge Univ. Press Presented by Britain.
  • El Hadrami, A., El Idrissi-Tourane, A., El Hassni, M. D. F., & El Hadrami, I. (2005). Toxin-Based in Vitro Selection and its Potential Application to Date Palm for Resistance to the Bayoud Fusarium wilt. C. R. Biologies. 328(8):732-744. https://doi.org/10.1016/j.crvi.2005.05.007
  • Emek, Y., & Erdağ, B. (2008). Bursa Siyahı (Ficus carica L. cv. Bursa Siyahı) İncir Çeşidinin in vitro Koşullarda NaCl Stresine Tepkileri. 19. Ulusal Biyoloji Kongresi, 23-27 Haziran, Trabzon.
  • Erişdi, H. (2015). Orta Anadolu Kökenli Mor Havuç Genotiplerinin Moleküler Karakterizasyonu. [Yayımlanmamış Yüksek Lisans Tezi]. Selçuk Üniversitesi.
  • Fujimura, T., & Komamine, A. (1979). Synchronization of Somatic Embryogenesis in a Carrot Cell Suspension Culture. Plant Physiol. 64, 162-164. https://doi.org/10.1104/pp.64.1.162
  • George, E.R., & Sherrington, P. D. (1984). Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories. Exegetics.
  • Herdem, S. (1998). Havuçta Hücre Süspansiyon Kültürü Yoluyla Somatik Embriyo Oluşumu Üzerinde Bir Araştırma. [Yayımlanmamış Yüksek Lisans Tezi]. Ankara Üniversitesi.
  • IAEA, (2022). Mutant Variety Database, Son Erişim: 15.05.2022. Link:https://mvd.iaea.org.
  • Ipek, A. (2002). Introduction of an Ac/Ds Based Two-Element Transposon Tagging System and Trans-Activation Of Ds In Carrot (Daucus carota L.) Doctor of Philosophy (Horticulture) University Of Wisconsin-Madison 185s, USA.
  • Kantoğlu, Y., Seçer, E., Erzurum, K., Kunter, B., Şekerci, S., Kayabaşı, N., Özçoban, M., Tutluer, İ., Peşkircioğlu, H., Sağel, Z., Maden, S., & Yanmaz, R. (2009). Kavun Solgunluk Etmeni Fusarium oxysporum f. sp. melonis Irk 1,2’e Karşı Doku Kültürü ve Mutasyon Teknikleri Kullanarak Dayanıklı Kavun Tiplerinin Seçilmesi Üzerinde Araştırmalar. X. Ulusal Nükleer Bilimler ve Teknolojileri Kongresi, 6-9 Ekim, 302-311.
  • Karataş, İ. (2013). Siyah Havuç (Daucus carota) Kallus ve Hücre Süspansiyon Kültüründe Antosiyanin üretimine Uv-C Stresinin ve Fiboflavinin Etkilerinin Belirlenmesi. [Yayımlanmamış Doktora Tezi]. Gaziosmanpaşa Üniversitesi.
  • Murashige, T., & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiol Plant. 15:473–97. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Montilla, E. C., Arzaba, M. R. Hillebrand, S.. & Winterhalter P, (2011). Anthocyanin composition of black carrot (Daucus carota ssp. sativus var. atrorubens Alef.) cultivars antonina, beta sweet, deep purple and purple haze. Journal of Agricultural and Food Chemistry. 59 pp. 3385-3390. https://doi.org/10.1021/jf104724k
  • Neumann, K. H., Kumar, A., & Imani, J. (2009). Plant Cell and Tissue Culture - A Tool in Biotechnology. Springer-Verlag, Berlin Heidelberg, 1-7.
  • Ozsan, T., Vural, G. E., & Onus, A. N. (2020). Do Jasmonic Acid and Activated Charcoal Increase the in vitro Development of Orange Carrot (Daucus carota L. subsp. sativus Hoffm.) and Purple Carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) Acta Sci. Pol. Hortorum Cultus. 19(5): 115–127. https://doi.org/10.24326/asphc.2020.5.12
  • Queiros, F., Fidalgo, F., Santos, I., & Salema, R. (2007). In vitro Selection of Salt Tolerant Cell Lines in Solanum tuberosum L. Biologia Plantarum. 51 (4): 728- 734. https://doi.org/10.1007/s10535-007-0149-y
  • Reinert, T. J. (1973). Aspects of Organization. Organogenesis and Embryogenesis, pp. 388-555
  • Rubatzky, V. E., Quiros, C. F., & Simon, P.W. (1999). Carrots and Related Vegetable Umbelliferae, Crop Production Science in Horticulture. CABI Publishing 7:1-8.
  • Rus, A. M., Segundo, R., Olmos, E., Santa-Cruz, A., Maria C., & Bolarin, M. C. (2000). Long-term Culture Modifies the Salt Responses Of Callus Lines Of Salt Tolerant and Salt-Sensitive Tomato Species J. Plant Physiol. 157: 413-420. https://doi.org/10.1016/S0176-1617(00)80026-7
  • Taner, K. Y. (2002). Plant Formation By Somatic Embryogenesis in Melon (Cucumis melo L.). [Unpublished PhD Thesis]. Ankara University.
  • Taner, Y., Beşirli, G., Kunter, B., & Yanmaz, R. (2004). Sarımsakta (Allium sativum L.) Radyasyonla Mutasyon Islahına Yönelik Olarak Etkili Mutasyon Dozunun Belirlenmesi. Bahçe. 33 (1-2):95-99.
  • Torres, K. C. (1989). Tissue Culture Techniques for Horticultural Crops. Published by Van Nostrand Reinhold. New York, 279 p.
  • Torun, A. A., Aka Kaçar, Y., Kıllı, O., Erdem, H., Yardım, P., Serçe, S., & İnan, S. (2007). Bazı Fragaria chiloensis Genotiplerinin Değişik NaCl Konsantrasyonlarına In Vitro Çoğaltma Sırasındaki Tepkileri, Batı Akdeniz Tarımsal Araştırma Enstitüsü Derim Dergisi. 24(2): 27–33.
  • Watad, A. A., Reinhold, L., & Lerner, H. R. (1983). Comparison Between a Stable NaCl Selected Nicotiana Cell Line and the Wild Type. Plant Physiol. 73: 624- 629. https://doi.org/10.1104/pp.73.3.624
  • Winicov, I. (1996). Characterization of Rice (Oryza sativa L.) Plants Regenerated from Salt-Tolerant Cell Lines. Plant Science. 113: 105-111. https://doi.org/10.1016/0168-9452(95)04274-1

Callus and suspension culture techniques optimized for use in carrot breeding studies (Daucus carota ssp. sativus var. atrorubens Alef and D. carota)

Year 2023, , 1 - 9, 30.06.2023
https://doi.org/10.38042/biotechstudies.1249244

Abstract

In this study, studies were conducted to optimize callus and suspension culture methods for in vitro mutation breeding in purple and orange carrots. Following this, the developed mutant lines were subjected to in vitro salt stress selection. The study determined the optimal agar dose. The first germination, 50% germination, root-cotyledon formation and genuine leaf formation in carrot seedlings were analyzed daily in the study, which was continued with the determined 7g/L agar dose. The cotyledon and hypocotyl explants from the seedlings were cultivated for callus production in mixtures of MS-1, MS-2 and MS-3 nutritional medium. In the second and fourth weeks following the second subculture, callus production percentages and weights were measured. The MS-3 (1 mg/L 2,4-D + 0.5 mg/L kinetin) nutritional medium and hypocotyl explant were found to be particularly effective at producing callus. The second subculture's data on the number of plants that had successfully regenerated per callus, showed that the MS nutritional medium with 0.2 mg/L Thidiazuron (TDZ) was the best medium for plant regeneration. The dispersed calli were grown in a nutritional medium designed for suspension culture in a nutrient medium mixture comprising MS+0.1 mg/L kinetin. The results obtained with the optimization steps were used in the ongoing study.

References

  • Arıcı, E. Ş. (2006). Somaklonal Varyasyondan Yararlanarak in vitro Seleksiyonla Buğday (Triticum aestivum L.)’da Başak Yanıklığına Dayanıklı Bitki Elde Edilmesi. [Yayımlanmamış Doktora Tezi]. Çukurova Üniversitesi.
  • Babaoğlu, M., Yorgancılar, M., & Akbudak, M. A. (2001). Doku Kültürü: Temel laboratuvar teknikleri, Bitki Biyoteknolojisi, Doku Kültürü ve Uygulamaları. Editörler: Babaoğlu, M. Gürel, E. Özcan, S. Konya, s.1-35.
  • Bado, S., Rafiri, M. A., El-Achouri, K., Sapey, E., Nielen, S., Ghanim, A. M. A., Forster, B.P., & Laimer, M. (2016). In vitro Methods for Mutation Induction in Potato (Solanum tuberosum L.). African Journal of Biotechnology 15(39): 2132-2145. https://doi.org/10.5897/AJB2016.15571
  • Ben-Hayyim, G., & Kochba, J. (1983). Aspects of Salt Tolerance in a NaCI-Selected Stable Cell Line of Citrus sinensis. Plant Physiol. 72: 685-690. https://doi.org/10.1104/pp.72.3.685
  • Bradley, P. M., El Fike, F., & Giles, K. L. (1984). Polyamines and Arginine Affect Somatic Embryogenesis of Daucus carota L. Plant Science Letters. 34: 397-401. https://doi.org/10.1016/S0304-4211(84)80021-8
  • Bükün, B., Gaines, T., Nissen, S. J., Westra, P., Shanner, D. L., Leach, J., Chisholm, S., Ward, S., Preston, C., Culpepper, S., Gray, T., Webster, T., Vencill, B., & Tranel, P. (2009). Amaranthus palmeri L.’nin Glyphosata Dayanıklılık Mekanizması. Türkiye III. Bitki Koruma Kongresi, 15-18 Temmuz, Van.
  • Dodds, J. H., & Roberts, L.W. (1982). Experiments in Plant Tissue Culture sec. Edi. Cambridge Univ. Press Presented by Britain.
  • El Hadrami, A., El Idrissi-Tourane, A., El Hassni, M. D. F., & El Hadrami, I. (2005). Toxin-Based in Vitro Selection and its Potential Application to Date Palm for Resistance to the Bayoud Fusarium wilt. C. R. Biologies. 328(8):732-744. https://doi.org/10.1016/j.crvi.2005.05.007
  • Emek, Y., & Erdağ, B. (2008). Bursa Siyahı (Ficus carica L. cv. Bursa Siyahı) İncir Çeşidinin in vitro Koşullarda NaCl Stresine Tepkileri. 19. Ulusal Biyoloji Kongresi, 23-27 Haziran, Trabzon.
  • Erişdi, H. (2015). Orta Anadolu Kökenli Mor Havuç Genotiplerinin Moleküler Karakterizasyonu. [Yayımlanmamış Yüksek Lisans Tezi]. Selçuk Üniversitesi.
  • Fujimura, T., & Komamine, A. (1979). Synchronization of Somatic Embryogenesis in a Carrot Cell Suspension Culture. Plant Physiol. 64, 162-164. https://doi.org/10.1104/pp.64.1.162
  • George, E.R., & Sherrington, P. D. (1984). Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories. Exegetics.
  • Herdem, S. (1998). Havuçta Hücre Süspansiyon Kültürü Yoluyla Somatik Embriyo Oluşumu Üzerinde Bir Araştırma. [Yayımlanmamış Yüksek Lisans Tezi]. Ankara Üniversitesi.
  • IAEA, (2022). Mutant Variety Database, Son Erişim: 15.05.2022. Link:https://mvd.iaea.org.
  • Ipek, A. (2002). Introduction of an Ac/Ds Based Two-Element Transposon Tagging System and Trans-Activation Of Ds In Carrot (Daucus carota L.) Doctor of Philosophy (Horticulture) University Of Wisconsin-Madison 185s, USA.
  • Kantoğlu, Y., Seçer, E., Erzurum, K., Kunter, B., Şekerci, S., Kayabaşı, N., Özçoban, M., Tutluer, İ., Peşkircioğlu, H., Sağel, Z., Maden, S., & Yanmaz, R. (2009). Kavun Solgunluk Etmeni Fusarium oxysporum f. sp. melonis Irk 1,2’e Karşı Doku Kültürü ve Mutasyon Teknikleri Kullanarak Dayanıklı Kavun Tiplerinin Seçilmesi Üzerinde Araştırmalar. X. Ulusal Nükleer Bilimler ve Teknolojileri Kongresi, 6-9 Ekim, 302-311.
  • Karataş, İ. (2013). Siyah Havuç (Daucus carota) Kallus ve Hücre Süspansiyon Kültüründe Antosiyanin üretimine Uv-C Stresinin ve Fiboflavinin Etkilerinin Belirlenmesi. [Yayımlanmamış Doktora Tezi]. Gaziosmanpaşa Üniversitesi.
  • Murashige, T., & Skoog, F. (1962). A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiol Plant. 15:473–97. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • Montilla, E. C., Arzaba, M. R. Hillebrand, S.. & Winterhalter P, (2011). Anthocyanin composition of black carrot (Daucus carota ssp. sativus var. atrorubens Alef.) cultivars antonina, beta sweet, deep purple and purple haze. Journal of Agricultural and Food Chemistry. 59 pp. 3385-3390. https://doi.org/10.1021/jf104724k
  • Neumann, K. H., Kumar, A., & Imani, J. (2009). Plant Cell and Tissue Culture - A Tool in Biotechnology. Springer-Verlag, Berlin Heidelberg, 1-7.
  • Ozsan, T., Vural, G. E., & Onus, A. N. (2020). Do Jasmonic Acid and Activated Charcoal Increase the in vitro Development of Orange Carrot (Daucus carota L. subsp. sativus Hoffm.) and Purple Carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) Acta Sci. Pol. Hortorum Cultus. 19(5): 115–127. https://doi.org/10.24326/asphc.2020.5.12
  • Queiros, F., Fidalgo, F., Santos, I., & Salema, R. (2007). In vitro Selection of Salt Tolerant Cell Lines in Solanum tuberosum L. Biologia Plantarum. 51 (4): 728- 734. https://doi.org/10.1007/s10535-007-0149-y
  • Reinert, T. J. (1973). Aspects of Organization. Organogenesis and Embryogenesis, pp. 388-555
  • Rubatzky, V. E., Quiros, C. F., & Simon, P.W. (1999). Carrots and Related Vegetable Umbelliferae, Crop Production Science in Horticulture. CABI Publishing 7:1-8.
  • Rus, A. M., Segundo, R., Olmos, E., Santa-Cruz, A., Maria C., & Bolarin, M. C. (2000). Long-term Culture Modifies the Salt Responses Of Callus Lines Of Salt Tolerant and Salt-Sensitive Tomato Species J. Plant Physiol. 157: 413-420. https://doi.org/10.1016/S0176-1617(00)80026-7
  • Taner, K. Y. (2002). Plant Formation By Somatic Embryogenesis in Melon (Cucumis melo L.). [Unpublished PhD Thesis]. Ankara University.
  • Taner, Y., Beşirli, G., Kunter, B., & Yanmaz, R. (2004). Sarımsakta (Allium sativum L.) Radyasyonla Mutasyon Islahına Yönelik Olarak Etkili Mutasyon Dozunun Belirlenmesi. Bahçe. 33 (1-2):95-99.
  • Torres, K. C. (1989). Tissue Culture Techniques for Horticultural Crops. Published by Van Nostrand Reinhold. New York, 279 p.
  • Torun, A. A., Aka Kaçar, Y., Kıllı, O., Erdem, H., Yardım, P., Serçe, S., & İnan, S. (2007). Bazı Fragaria chiloensis Genotiplerinin Değişik NaCl Konsantrasyonlarına In Vitro Çoğaltma Sırasındaki Tepkileri, Batı Akdeniz Tarımsal Araştırma Enstitüsü Derim Dergisi. 24(2): 27–33.
  • Watad, A. A., Reinhold, L., & Lerner, H. R. (1983). Comparison Between a Stable NaCl Selected Nicotiana Cell Line and the Wild Type. Plant Physiol. 73: 624- 629. https://doi.org/10.1104/pp.73.3.624
  • Winicov, I. (1996). Characterization of Rice (Oryza sativa L.) Plants Regenerated from Salt-Tolerant Cell Lines. Plant Science. 113: 105-111. https://doi.org/10.1016/0168-9452(95)04274-1
There are 31 citations in total.

Details

Primary Language English
Subjects Botany, Agricultural Engineering
Journal Section Research Articles
Authors

Damla Turan Büyükdinç

Yaprak Kantoğlu This is me

Arzu Karataş This is me

Şeküre Şebnem Ellialtıoğlu This is me

Early Pub Date June 5, 2023
Publication Date June 30, 2023
Published in Issue Year 2023

Cite

APA Turan Büyükdinç, D., Kantoğlu, Y., Karataş, A., Ellialtıoğlu, Ş. Ş. (2023). Callus and suspension culture techniques optimized for use in carrot breeding studies (Daucus carota ssp. sativus var. atrorubens Alef and D. carota). Biotech Studies, 32(1), 1-9. https://doi.org/10.38042/biotechstudies.1249244


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