Research Article

Microencapsulation of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12 in alginate and pectin, and the effects on bacterial acid and bile resistance

Number: 35 March 5, 2026

Microencapsulation of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12 in alginate and pectin, and the effects on bacterial acid and bile resistance

Abstract

This study aimed to encapsulate Lactobacillus acidophilus LA-5 (LA-5) and Bifidobacterium animalis subsp. lactis BB-12 (BB-12) in alginate and pectin, and to determine the effects of acid and bile resistance. The emulsion method was used; different gel and CaCl2 concentrations and stirring rate were studied comparatively for the characterization of microcapsules. Alginate microcapsules were smaller and exhibited agglomeration behavior, while pectin microcapsules were fairly homogeneous and had a smooth shape. Encapsulation efficiency (EE) percentages of the bacteria cells encapsulated with alginate and pectin varied within a very narrow range of 83 to 85%. The size of microcapsules increased as the gel concentration increased, and decreased as the CaCl2 concentration and stirring rate increased. Microencapsulated cells had more acid and bile resistance than free cells. No significant difference was found between alginate and pectin microcapsules of the two strains in terms of acid and bile resistance. The probiotic strains encapsulated with pectin and alginate maintained higher levels of viability under acid conditions at pH 2 and 3 than at pH 1 after 24 hours of incubation.

Keywords

Supporting Institution

This study was supported by Coordinatorship of Scientific Research Projects of Hacettepe University (Project number: 0601602009).

Project Number

Project number: 0601602009

Thanks

The authors are grateful to Coordinatorship of Scientific Research Projects of Hacettepe University for the financial support.

References

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Details

Primary Language

English

Subjects

Industrial Microbiology, Enzyme and Microbial Biotechnology in Agriculture

Journal Section

Research Article

Early Pub Date

December 26, 2025

Publication Date

March 5, 2026

Submission Date

February 18, 2025

Acceptance Date

November 10, 2025

Published in Issue

Year 2026 Number: 35

APA
Mumcu, A., Temiz, A., & Denkbas, E. B. (2026). Microencapsulation of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12 in alginate and pectin, and the effects on bacterial acid and bile resistance. Biotech Studies, 35, 1849883. https://doi.org/10.38042/biotechstudies.1849883
AMA
1.Mumcu A, Temiz A, Denkbas EB. Microencapsulation of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12 in alginate and pectin, and the effects on bacterial acid and bile resistance. Biotech Studies. 2026;(35):1849883. doi:10.38042/biotechstudies.1849883
Chicago
Mumcu, Ayla, Ayhan Temiz, and Emir Baki Denkbas. 2026. “Microencapsulation of Lactobacillus Acidophilus LA-5 and Bifidobacterium Animalis Subsp. Lactis BB-12 in Alginate and Pectin, and the Effects on Bacterial Acid and Bile Resistance”. Biotech Studies, nos. 35: 1849883. https://doi.org/10.38042/biotechstudies.1849883.
EndNote
Mumcu A, Temiz A, Denkbas EB (March 1, 2026) Microencapsulation of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12 in alginate and pectin, and the effects on bacterial acid and bile resistance. Biotech Studies 35 1849883.
IEEE
[1]A. Mumcu, A. Temiz, and E. B. Denkbas, “Microencapsulation of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12 in alginate and pectin, and the effects on bacterial acid and bile resistance”, Biotech Studies, no. 35, p. 1849883, Mar. 2026, doi: 10.38042/biotechstudies.1849883.
ISNAD
Mumcu, Ayla - Temiz, Ayhan - Denkbas, Emir Baki. “Microencapsulation of Lactobacillus Acidophilus LA-5 and Bifidobacterium Animalis Subsp. Lactis BB-12 in Alginate and Pectin, and the Effects on Bacterial Acid and Bile Resistance”. Biotech Studies. 35 (March 1, 2026): 1849883. https://doi.org/10.38042/biotechstudies.1849883.
JAMA
1.Mumcu A, Temiz A, Denkbas EB. Microencapsulation of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12 in alginate and pectin, and the effects on bacterial acid and bile resistance. Biotech Studies. 2026;:1849883.
MLA
Mumcu, Ayla, et al. “Microencapsulation of Lactobacillus Acidophilus LA-5 and Bifidobacterium Animalis Subsp. Lactis BB-12 in Alginate and Pectin, and the Effects on Bacterial Acid and Bile Resistance”. Biotech Studies, no. 35, Mar. 2026, p. 1849883, doi:10.38042/biotechstudies.1849883.
Vancouver
1.Ayla Mumcu, Ayhan Temiz, Emir Baki Denkbas. Microencapsulation of Lactobacillus acidophilus LA-5 and Bifidobacterium animalis subsp. lactis BB-12 in alginate and pectin, and the effects on bacterial acid and bile resistance. Biotech Studies. 2026 Mar. 1;(35):1849883. doi:10.38042/biotechstudies.1849883


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