Research Article

Effect of acetate as a substrate on the microbial flora: a metagenomic approach

Number: 35 June 29, 2026

Effect of acetate as a substrate on the microbial flora: a metagenomic approach

Abstract

Acetates possess broad utility across diverse sectors including food, industry, concrete production, and medical applications. In this study, two different Winogradsky columns were prepared from muddy waters collected from Lake Akkaya (Nigde-Turkiye). Acetate was used as substrate in one of two mineral media in order to understand its impact on microbial inoculant from column under light and at room temperature for metagenomic studies. The results indicated that, the supplementation of acetate limited the growth of several members of the Proteobacteria phylum and reduced overall microbial diversity. Conversely, it has promoted the growth of various phototrophic and photoheterotrophic bacteria, particularly purple non-sulfur photosynthetic bacteria. In the absence of acetate, the most dominant bacterial species were Phyllobacterium members (34.97%), Aquimonas (13.32%), and Pandoraea (9.78%) respectively. In the acetate-supplemented medium, Rhodoplanes (35.15%), Pelolinea (25.53%), and Tiocapsa (6.87%) were predominant. These findings suggest that acetate promoted the growth of photosynthetic purple bacteria capable of utilizing aromatic compounds, indicating potential environmental benefits. Furthermore, acetate-supplementation may improve the efficiency wastewater treatment processes.

Keywords

Thanks

The authors are thankful to Prof. Meysun Ibrahim Abdullah for his valuable suggestions.

References

  1. Abdelsalam, N. A., Hegazy, S. M., Aziz, R. K. (2023). The curious case of Prevotella copri. Gut Microbes, 15, 2249152. https://doi.org/10.1080/19490976.2023.2249152
  2. Abdullah, M. I., Öztürk, A., Bayol, E. (2021). Biosorption of astrazon red dye by the bacterium Rhodopseudomonas sp. strain 51ATA. Environ. Earth Sci., 80, 49. https://doi.org/10.1007/s12665-020-09314-7
  3. Aggarwal, N., Kitano, S., Puah, G. R. Y., Kittelmann, S., Hwang, I. Y., Chang, M. W. (2023). Microbiome and Human Health: Current understanding, engineering, and enabling technologies. Chem. Rev., 123, 31–72. https://doi.org/10.1021/acs.chemrev.2c00431
  4. Allegue, L. D., Puyol, D., Melero, J. A. (2020). Food waste valorization by purple phototrophic bacteria and anaerobic digestion after thermal hydrolysis. Biomass Bioenergy, 142, 105803. https://doi.org/10.1016/j.biombioe.2020.105803
  5. Ayilara, M. S., Babalola, O. O. (2023). Bioremediation of environmental wastes: The role of microorganisms. Front. Agron., 5, 1183691. https://doi.org/10.3389/fagro.2023.1183691
  6. Barbosa, M. J., Rocha, J. M. S., Tramper, J., Wijffels, R. H. (2001). Acetate as a carbon source for hydrogen production by photosynthetic bacteria. J. Biotechnol., 85, 25–33. https://doi.org/10.1016/S0168-1656(00)00368-0
  7. Bolyen, E., Rideout, J. R., Dillon, M. R., Bokulich, N. A., Abnet, C. C., Al-Ghalith, G. A., ... and Caporaso, J. G. (2019). Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nature Biotechnology, 37, 852-857. https://doi.org/10.1038/s41587-019-0209-9
  8. Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A., and Holmes, S. P. (2016). DADA2: High-resolution sample inference from Illumina amplicon data. Nature Methods, 13(7), 581-583. https://doi.org/10.1038/nmeth.3869

Details

Primary Language

English

Subjects

Microbial Genetics

Journal Section

Research Article

Publication Date

June 29, 2026

Submission Date

May 11, 2025

Acceptance Date

June 16, 2026

Published in Issue

Year 2026 Number: 35

APA
Fetoshi, O., & Öztürk, A. (2026). Effect of acetate as a substrate on the microbial flora: a metagenomic approach. Biotech Studies, 35, 1981142. https://doi.org/10.38042/biotechstudies.1981142
AMA
1.Fetoshi O, Öztürk A. Effect of acetate as a substrate on the microbial flora: a metagenomic approach. Biotech Studies. 2026;(35):1981142. doi:10.38042/biotechstudies.1981142
Chicago
Fetoshi, Osman, and Ayten Öztürk. 2026. “Effect of Acetate As a Substrate on the Microbial Flora: A Metagenomic Approach”. Biotech Studies, nos. 35: 1981142. https://doi.org/10.38042/biotechstudies.1981142.
EndNote
Fetoshi O, Öztürk A (June 1, 2026) Effect of acetate as a substrate on the microbial flora: a metagenomic approach. Biotech Studies 35 1981142.
IEEE
[1]O. Fetoshi and A. Öztürk, “Effect of acetate as a substrate on the microbial flora: a metagenomic approach”, Biotech Studies, no. 35, p. 1981142, June 2026, doi: 10.38042/biotechstudies.1981142.
ISNAD
Fetoshi, Osman - Öztürk, Ayten. “Effect of Acetate As a Substrate on the Microbial Flora: A Metagenomic Approach”. Biotech Studies. 35 (June 1, 2026): 1981142. https://doi.org/10.38042/biotechstudies.1981142.
JAMA
1.Fetoshi O, Öztürk A. Effect of acetate as a substrate on the microbial flora: a metagenomic approach. Biotech Studies. 2026;:1981142.
MLA
Fetoshi, Osman, and Ayten Öztürk. “Effect of Acetate As a Substrate on the Microbial Flora: A Metagenomic Approach”. Biotech Studies, no. 35, June 2026, p. 1981142, doi:10.38042/biotechstudies.1981142.
Vancouver
1.Osman Fetoshi, Ayten Öztürk. Effect of acetate as a substrate on the microbial flora: a metagenomic approach. Biotech Studies. 2026 Jun. 1;(35):1981142. doi:10.38042/biotechstudies.1981142


ULAKBIM TR Index, Scopus, Google Scholar, Crossref, Scientific Indexing Services