Research Article

Analysis of secondary metabolites produced by Aspergillus niger and Bacillus sp. isolated from Wupa Wastewater Treatment Plant, Abuja

Number: 35 August 21, 2026
  • Umar Faruok Ibrahim *
  • Kabir Adamu
  • Sani Sambo Datsugwai Mohammed
  • Abdullahi Hassan Ndanusa
  • Aliyu-Paiko Mohammed

Analysis of secondary metabolites produced by Aspergillus niger and Bacillus sp. isolated from Wupa Wastewater Treatment Plant, Abuja

Abstract

Aquatic pollution is caused by the rapid development in industrialization. Therefore, using microorganisms to remediate industrial wastewater (IWW) is a green method that would stop or lessen aquatic contamination. This work explores the secondary metabolites generated by Bacillus sp. and Aspergillus niger that were identified from the Wupa Wastewater Treatment Plant in Abuja, Nigeria. Using morphological and molecular methods, the isolates were identified. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the secondary metabolites that the isolates generated. The extracts were extracted using liquid-liquid extraction. Potato Dextrose Agar and Nutrient Agar was supplemented with three different concentrations of each metal (5 to 80 mgL-1) Aspergillus niger and Bacillus sp. were selected for their potential to remediate heavy metal. Additionally, peptone broth was added with various heavy metal concentrations. By utilizing a UV spectrophotometer set at 600 nm to track the development of biomass, the remediation potential was determined. The results revealed a diverse array of bioactive compounds, including Octadec-9-enoic acid (27.16%), Butanoic acid (18.54%), Propanal, 3-methoxy- (9.33%), Oleoyl chloride (10.64%), n-Hexadecanoic acid (10.59), 2,3-Butanediol (6.92%), Octadec-9-enoic acid (16.28%) and other metabolites which have shown strong bioremediation potential applications in biotechnology and environmental management. The maximum potential for bioremediation was shown by Aspergillus niger and Bacillus sp., with optical densities of 1.080 in 40 mg FeL-1 and 10 mg ZnL-1, respectively. The importance of wastewater ecosystems as sources of beneficial secondary metabolites and their potential for heavy metal remediation are highlighted by this study.

Keywords

Thanks

The Authors wish to thank Ibrahim Badamasi Babngida University Lapai, Nile University of Nigeria, Abuja and Abuja Environmental Protection Agency for providing the necessary resources and facilities for conducting this research.

References

  1. Abioye, O. P., Oyewole, O. A., Oyeleke, S. B., Adeyemi, M., & Orukotan, A. (2018). Biosorption of lead, chromium and cadmium in tannery effluent using indigenous microorganisms. Braz J Biol Sci, 5, 25-32. https://doi.org/10.21472/bjbs.050903.
  2. Acosta-Rodríguez, I., Cárdenas-González, J. F., Rodríguez Pérez, A. S., Oviedo, J. T., & Martínez-Juárez, V. M. (2018). Bioremoval of different heavy metals by the resistant fungal strain Aspergillus niger. Bioinorganic chemistry and applications, 2018(1), 3457196. https://doi.org/10.1155/2018/3457196
  3. Akob, D. M., Mills, H. J., & Kostka, J. E. (2007). Metabolically active microbial communities in uranium-contaminated subsurface sediments. FEMS Microbiol Ecol 59(1). 95-107. https://doi.org/10.1111/j.1574-6941.2006.00203.x
  4. Al-Dhabi, N. A., Esmail, G. A., Mohammed-Ghilan, A. K., & Valan-Arasu, M. J. (2019). Optimizing the management of cadmium bioremediation capacity of metal-resistant Pseudomonas sp. strain Al-Dhabi-126 isolated from the industrial city of Saudi Arabian environment. IJEnvH. 16(23), 4788. https://doi.org/10.3390/ijerph16234788
  5. Al-Hawash, A.B., Zhang, J., Li, S., Liu, J., Ghalib, H. B., Zhang, X., & Ma, F. (2018). Biodegradation of n-hexadecane by Aspergillus sp. RFC-1 and its mechanism. Ecotoxicol Environ Saf. 164, 398-408. https://doi.org/10.1016/j.ecoenv.2018.08.049
  6. Alghamdi, M. D., Alshahrani, A. A., Alqarni, L. S., Alghamdi, S. Q., Moustafa, S. M. N., & Nassar, A. M. (2024). Chapter 2 - Green approaches in synthesis of surfactants. In: Manjunatha, J. G., & Hussain C. M. (Eds). Surfactant Based Electrochemical Sensors and Biosensors (pp. 21-68). Elsevier.
  7. Al-Imara, E. & Al-Gazzawy, G. (2020). The study of purified secondary metabolites extracts of Bacillus subtilis and its chemotaxis effect on biofilm-forming bacteria. J DYSONA Life Sci, 1(1), 1-13. https://doi.org/10.30493/dls.2020.103720
  8. Ali, B., Anushka, A., & Mishra A. (2022). Effects of dissolved oxygen concentration on freshwater fish: A review. Int J Fish Aquat, 10(4), 113-127. https://doi.org/10.22271/fish.2022.v10.i4b.2693

Details

Primary Language

English

Subjects

Industrial Microbiology, Environmentally Sustainable Engineering

Journal Section

Research Article

Authors

Umar Faruok Ibrahim * This is me
0000-0002-8463-6115
Nigeria

Sani Sambo Datsugwai Mohammed This is me
0000-0002-3879-6740
Nigeria

Abdullahi Hassan Ndanusa This is me
0000-0001-8411-0044
Nigeria

Aliyu-Paiko Mohammed This is me
0000-0003-3400-7588
Nigeria

Publication Date

August 21, 2026

Submission Date

July 12, 2026

Acceptance Date

August 3, 2026

Published in Issue

Year 2026 Number: 35

APA
Ibrahim, U. F., Adamu, K., Mohammed, S. S. D., Ndanusa, A. H., & Mohammed, A.-P. (2026). Analysis of secondary metabolites produced by Aspergillus niger and Bacillus sp. isolated from Wupa Wastewater Treatment Plant, Abuja. Biotech Studies, 35, 2023369. https://doi.org/10.38042/biotechstudies.2023369
AMA
1.Ibrahim UF, Adamu K, Mohammed SSD, Ndanusa AH, Mohammed AP. Analysis of secondary metabolites produced by Aspergillus niger and Bacillus sp. isolated from Wupa Wastewater Treatment Plant, Abuja. Biotech Studies. 2026;(35):2023369. doi:10.38042/biotechstudies.2023369
Chicago
Ibrahim, Umar Faruok, Kabir Adamu, Sani Sambo Datsugwai Mohammed, Abdullahi Hassan Ndanusa, and Aliyu-Paiko Mohammed. 2026. “Analysis of Secondary Metabolites Produced by Aspergillus Niger and Bacillus Sp. Isolated from Wupa Wastewater Treatment Plant, Abuja”. Biotech Studies, nos. 35: 2023369. https://doi.org/10.38042/biotechstudies.2023369.
EndNote
Ibrahim UF, Adamu K, Mohammed SSD, Ndanusa AH, Mohammed A-P (August 1, 2026) Analysis of secondary metabolites produced by Aspergillus niger and Bacillus sp. isolated from Wupa Wastewater Treatment Plant, Abuja. Biotech Studies 35 2023369.
IEEE
[1]U. F. Ibrahim, K. Adamu, S. S. D. Mohammed, A. H. Ndanusa, and A.-P. Mohammed, “Analysis of secondary metabolites produced by Aspergillus niger and Bacillus sp. isolated from Wupa Wastewater Treatment Plant, Abuja”, Biotech Studies, no. 35, p. 2023369, Aug. 2026, doi: 10.38042/biotechstudies.2023369.
ISNAD
Ibrahim, Umar Faruok - Adamu, Kabir - Mohammed, Sani Sambo Datsugwai - Ndanusa, Abdullahi Hassan - Mohammed, Aliyu-Paiko. “Analysis of Secondary Metabolites Produced by Aspergillus Niger and Bacillus Sp. Isolated from Wupa Wastewater Treatment Plant, Abuja”. Biotech Studies. 35 (August 1, 2026): 2023369. https://doi.org/10.38042/biotechstudies.2023369.
JAMA
1.Ibrahim UF, Adamu K, Mohammed SSD, Ndanusa AH, Mohammed A-P. Analysis of secondary metabolites produced by Aspergillus niger and Bacillus sp. isolated from Wupa Wastewater Treatment Plant, Abuja. Biotech Studies. 2026;:2023369.
MLA
Ibrahim, Umar Faruok, et al. “Analysis of Secondary Metabolites Produced by Aspergillus Niger and Bacillus Sp. Isolated from Wupa Wastewater Treatment Plant, Abuja”. Biotech Studies, no. 35, Aug. 2026, p. 2023369, doi:10.38042/biotechstudies.2023369.
Vancouver
1.Umar Faruok Ibrahim, Kabir Adamu, Sani Sambo Datsugwai Mohammed, Abdullahi Hassan Ndanusa, Aliyu-Paiko Mohammed. Analysis of secondary metabolites produced by Aspergillus niger and Bacillus sp. isolated from Wupa Wastewater Treatment Plant, Abuja. Biotech Studies. 2026 Aug. 1;(35):2023369. doi:10.38042/biotechstudies.2023369


ULAKBIM TR Index, Scopus, Google Scholar, Crossref, Scientific Indexing Services