Development of doubled haploid maize lines by using in vivo haploid technique

Year 2020, , 1 - 7, 30.06.2020

Rahime Cengiz Kayıhan Zahit Korkut

https://doi.org/10.38042/biost.2020.29.01.01

Cited By: 4

Abstract

The doubled haploid technology is now an integral component of modern maize breeding programs. In this study, the maternal haploid induction (gynogenesis) method was used to derive Doubled-Haploid (DH) lines from elite maize germplasm adapted to Turkey. Temperate haploid inducers (RWS, RWK-76, RWS x RWK-76 and WS14) were used as pollinators, and a set of 30 single-crossses (in FAO 650-700 maturity groups) were used as source materials. Putative haploid seeds were selected based on expression of R1-nj anthocyanin color marker. Highest haploid induction rate (20.42%) was recorded by using RWK-76 as inducer line, and the lowest haploid induction rate (17.75%) was obtained through WS14. Putative haploid seeds were germinated and seedlings were treated with 0.06% colchicine + 0.5% dimethylsulfoxide solution. Following transfer of seedlings into the field, 2178 D0 plants were obtained out of a total of 3012 treated haploids. Live plants were from 89% of 2178 seedlings which are planted to the field. Fertile plants were formed 57% of live plants. Inbreeding was succeeded in 31.23% of fertile plants and only 7.8% of inbreeding plants were able to produce seeds. Consequently, 27 doubled haploid lines were developed.

Keywords

Breeding, Chromosome doubling, Doubled haploid, In vivo maternal haploid, Maize

References

• Altuntaş, Y., Kocamaz, A. F., Cengiz, R. & Esmeray, M. (2018). Classification of Haploid and Diploid Maize Seeds Using by Image Processing Techniques and Support Vector Machines, 2018 26th Signal Processing and Communications Applications Conference (SIU), İzmir.
• Barnabás, B., Obert, B. & Kovács, G. (1999). Colchicine, an efficient genome-doubling agent for maize (Zea mays L.) microspores cultured in anthero. Plant Cell Reports, 18, 858-862.
• Cengiz, R., Cerit, İ., Tezel, M. & Pamukçu, M. (2013). Kendilenmiş hatların elde edilmesi. In Melez Mısırla 100 Yıl Çalıştayı Kitabı (p. 115-136). Ankara: BİSAB.
• Chaikam, V. & Prasanna, B. M. (2012). Maternal haploid detection using anthocyanin markers. In: Prasanna, B. M., Chaikam, V. & Mahukued, G. (eds.), Doubled Haploid Technology in Maize Breeding: Theory and Practice (p. 21-24). CIMMYT, Mexico, DF.
• Chase, S. S. (1951). Production of homozygous diploids of maize from monoploids. Agronomy, 44, 263-267.
• Coe, E. H. & Sarkar, K. R. (1964). The detection of haploids in maize. J. Heredity 55, 231-233.
• Deimling, S., Röber, F. & Geiger, H. H. (1997). Methodik und Genetik der in-vivo-Haploideninduktion bei Mais. Vortr Pflanzenzüchtung, 38, 203-224.
• Gallais, A. (1990). Quantitative genetics of doubled haploid populations and application to the theory of line development. Genetics, 124, 199-206.
• Geiger, H. H. (2009). Doubled haploids. In Bennetzen, J. L. & Hake, S. (eds.), Handbook of Maize. Springer, New York, NY.
• Lashermes, P. & Beckert, M. (1988). Genetic control of maternal haploidy in maize (Zea mays L.) and selection of haploid inducing lines. Theoretical and Applied Genetics, 76, 405-410.
• Melchinger, A. E., Schipprack, W., Friedrich, H. U. & Mirdita, V. (2014). In vivo haploid induction in maize: identification of haploid seeds by their oil content. Crop Science, 54(4), 1497-1504.
• Prigge, V., Sanchez, C., Dhillon, B. S., Schipprack, W., Araus, J. L., Banziger, M. & Melchinger, A. E. (2011). Doubled haploids in tropical maize: I. Effects of inducers and source germplasm on in vivo haploid induction rates. Crop Science, 51, 1498-1506.
• Rotarenco, V. A., Kirtoca, I. H. & Jacota, A. G. (2007). Possibility to identify kernels with haploid embryo by oil content. Maize Genetics Cooperation Newsletter, 81(11).
• Rotarenco, V. A., Dicu, G., State, D. & Fuia, S. (2010). New inducers of maternal haploids in maize. Maize Genetics Cooperation Newsletter, 84, 15.
• Röber, F. K., Gordillo, G. A. & Geiger, H. H. (2005). In vivo haploid induction in maize-performance of new inducers and significance of doubled haploid lines in hybrid breeding. Maydica, 50, 275-283.
• Spitkó, T., Sági, L., Pintér, J., Marton, L. C. & Barnabás, B. (2006). Haploid regeneration aptitude maize (Zea mays L.) lines of various origin and of their hybrids. Maydica, 51, 537-542.