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Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen

Year 2020, , 55 - 61, 30.12.2020
https://doi.org/10.38042/biost.2020.29.02.01

Abstract

In the present study, three entomopathogenic strains of Bacillus sphaericus (MBI5, MBI6, and MBI7) were isolated from greenhouse pest and larval habitat of Istanbul, Turkey. All of the MBI groups tested in laboratory bioassays were able to kill larvae, but not pupae and adults of Culex spp. in water. The most efficient larvicidal activity of the strains was found in the presence of two toxin genes, binA and binB for MBI5, MBI6, and MBI7 strains. Each B. sphaericus strains identified in this study were characterized as unique and novel in terms of fatty acid methyl ester (FAME) profiles and 16S rRNA sequencing data. The results of this study suggest that the three strains of B. sphaericus may be new sources of potential biocontrol agent of mosquito.

References

  • Berry, C. (2012). The bacterium, Lysinibacillus sphaericus, as an insect pathogen. Journal of Invertebrate Pathology, 109(1), 1-10.
Year 2020, , 55 - 61, 30.12.2020
https://doi.org/10.38042/biost.2020.29.02.01

Abstract

References

  • Berry, C. (2012). The bacterium, Lysinibacillus sphaericus, as an insect pathogen. Journal of Invertebrate Pathology, 109(1), 1-10.
There are 1 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Research Articles
Authors

Münevver Müge Çağal 0000-0002-1786-1216

Ali Umman Doğan This is me 0000-0002-9373-2766

Fatih Çakar This is me

Fikrettin Şahin This is me 0000-0002-9793-1455

Publication Date December 30, 2020
Published in Issue Year 2020

Cite

APA Çağal, M. M., Doğan, A. U., Çakar, F., Şahin, F. (2020). Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen. Biotech Studies, 29(2), 55-61. https://doi.org/10.38042/biost.2020.29.02.01


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