Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen

Münevver Müge Çağal; Ali Umman Doğan; Fatih Çakar; Fikrettin Şahin

doi:10.38042/biost.2020.29.02.01

EN

Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen

Abstract

In the present study, three entomopathogenic strains of Bacillus sphaericus (MBI5, MBI6, and MBI7) were isolated from greenhouse pest and larval habitat of Istanbul, Turkey. All of the MBI groups tested in laboratory bioassays were able to kill larvae, but not pupae and adults of Culex spp. in water. The most efficient larvicidal activity of the strains was found in the presence of two toxin genes, binA and binB for MBI5, MBI6, and MBI7 strains. Each B. sphaericus strains identified in this study were characterized as unique and novel in terms of fatty acid methyl ester (FAME) profiles and 16S rRNA sequencing data. The results of this study suggest that the three strains of B. sphaericus may be new sources of potential biocontrol agent of mosquito.

Keywords

References

Berry, C. (2012). The bacterium, Lysinibacillus sphaericus, as an insect pathogen. Journal of Invertebrate Pathology, 109(1), 1-10.

Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Münevver Müge Çağal ^*
0000-0002-1786-1216
Türkiye

Ali Umman Doğan This is me
0000-0002-9373-2766
Türkiye

Fatih Çakar This is me
United States

Fikrettin Şahin This is me
0000-0002-9793-1455
Türkiye

Publication Date

December 30, 2020

Submission Date

March 25, 2020

Acceptance Date

September 13, 2020

Published in Issue

Year 2020 Volume: 29 Number: 2

DOI

https://doi.org/10.38042/biost.2020.29.02.01

IZ

https://izlik.org/JA22KB78HY

Cite

RIS / Bibtex

APA

Çağal, M. M., Doğan, A. U., Çakar, F., & Şahin, F. (2020). Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen. Biotech Studies, 29(2), 55-61. https://doi.org/10.38042/biost.2020.29.02.01

AMA

1.Çağal MM, Doğan AU, Çakar F, Şahin F. Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen. Biotech Studies. 2020;29(2):55-61. doi:10.38042/biost.2020.29.02.01

Chicago

Çağal, Münevver Müge, Ali Umman Doğan, Fatih Çakar, and Fikrettin Şahin. 2020. “Isolation, Identification and Characterization of Three New Strains of Bacillus Sphaericus As Mosquito Pathogen”. Biotech Studies 29 (2): 55-61. https://doi.org/10.38042/biost.2020.29.02.01.

EndNote

Çağal MM, Doğan AU, Çakar F, Şahin F (December 1, 2020) Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen. Biotech Studies 29 2 55–61.

IEEE

[1]M. M. Çağal, A. U. Doğan, F. Çakar, and F. Şahin, “Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen”, Biotech Studies, vol. 29, no. 2, pp. 55–61, Dec. 2020, doi: 10.38042/biost.2020.29.02.01.

ISNAD

Çağal, Münevver Müge - Doğan, Ali Umman - Çakar, Fatih - Şahin, Fikrettin. “Isolation, Identification and Characterization of Three New Strains of Bacillus Sphaericus As Mosquito Pathogen”. Biotech Studies 29/2 (December 1, 2020): 55-61. https://doi.org/10.38042/biost.2020.29.02.01.

JAMA

1.Çağal MM, Doğan AU, Çakar F, Şahin F. Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen. Biotech Studies. 2020;29:55–61.

MLA

Çağal, Münevver Müge, et al. “Isolation, Identification and Characterization of Three New Strains of Bacillus Sphaericus As Mosquito Pathogen”. Biotech Studies, vol. 29, no. 2, Dec. 2020, pp. 55-61, doi:10.38042/biost.2020.29.02.01.

Vancouver

1.Münevver Müge Çağal, Ali Umman Doğan, Fatih Çakar, Fikrettin Şahin. Isolation, identification and characterization of three new strains of Bacillus sphaericus as mosquito pathogen. Biotech Studies. 2020 Dec. 1;29(2):55-61. doi:10.38042/biost.2020.29.02.01