Obtaining haploid plants by irradiated pollen culture in oil seed crops

Year 2023, Volume: 32 Issue: 1, 10 - 16, 30.06.2023

Hümeyra Yaman Nesrin Karaca Sanyürek

https://doi.org/10.38042/biotechstudies.1273824

Cited By: 1

Abstract

Haploid plant production is of great importance to shorten the breeding period in plant breeding programs. Obtaining pure lines in plant growing programs require an intensive work with huge labor and time. Obtaining one hundred percent homozy-gous pure lines is a key point for the improvement and development of new cultivars. Haploid plants with a single set of homozygous chromosomes have become a valua-ble tool in plant breeding. Dihaploid plants that are homozygous at all loci with dou-bling of their chromosomes can be propagated by seed and reach full homozygosity in a single generation. Traditional methods take seven years to reach homozygosity. Dihaploidization methods provide significant advantages in terms of gaining homo-zygosity in a short period of one year and bringing pure lines into agriculture. Anther culture and irradiated pollen technique are among the most widely used techniques in this respect; where physical or chemical agents are used to induce mutated pollen grains and anthers that are subsequently employed to develop dihaploids through in vitro cultures. These techniques are a good source to facilitate gene mapping, cyto-genetic research, and evolutionary studies. Irradiated pollen culture techniques have been applied to many oilseed crops to obtain pure lines. This study highlights some salient features of producing dihaploids using irradiated pollen grains and their maintenance.

Keywords

Biotechnology, Pure line, Dihaploidization

References

• Ahmad, I., Day, J. P., Macdonald, M. V., & Ingram, D. S. (1991). Haploid culture and UV mutagenesis in rapid-cycling Brassica napus for the generation of resistance to chlorsulfuron and Alternaria brassicicola. Annals of Botany, 67(6), 519-521. https://doi.org/10.1093/oxfordjournals.aob.a088193
• Aktaş, Y. E. (2018). Induction of haploid plants in sunflower (Helianthus annuus L.) by pollen irradiation. [Yayım-lanmamış Yüksek Lisans Tezi]. Marmara Üniversitesi.
• Aktaş, Y. E., Uncuoğlu, A. A., & Aydin, Y. (2018, September, 2-5). Induction of Parthenogenetic Haploid Embryos and Plants After Pollination by Irradiated Pollen in Sunflo-wer. International Agricultural Biological Life Science Conference, Edirne, Türkiye.
• Al-Safadi, B., & Simon, P. (1990). The effects of gamma irradiation on the growth and cytology of carrot (Daucus carota L.) tissue culture. Environmental and Experimen-tal Botany, 30(3), 361-371. https://doi.org/10.12691/ajfst-1-3-5
• Amri-Tiliouine, W., Laouar, M., Abdelguerfi, A., Jankowicz-Cieslak, J., Jankulosk, L., & Till, B. J. (2018). Genetic Va-riability Induced by Gamma Rays and Preliminary Re-sults of Low-Cost Tıllıng on M2 Generation of Chickpea (Cicer arietinum L.). Frontiers in Plant Science, 9, 1568. https://doi.org/10.3389/fpls.2018.01568
• Arıoğlu, H. H., Kolsarıcı, Ö., Göksu, A. T., Güllüoğlu, L., Arslan, M., Çalışkan, S. (2010, January,11-15). Yağ Bitkileri Üre-timinin Arttırılması Olanakları. Ziraat Mühendisligi VII. Teknik Kongresi, Ankara, Türkiye.
• Arıoğlu, H., Kolsarıcı, Ö., Kurt, O., Çalışkan, S., Aslan, M., İşler, N., Göksoy, A. T., Başalma, D., Baydar, H., Özer, H., Uzun, B., Önemli, F., Kaya, Y., Sincik, M., Öztürk, Ö., Kıl-lı, F., Tunçtürk, R., Öztürk, E., İlker, E., Aslanoğlu, F., Ay-taç, S., Onat, B., Kurt, C., Çubukçu, P., Bakal, H. (2020, January, 13-17). Yağlı Tohumlar Üretiminde Mevcut Du-rum ve Gelecek. Türkiye Ziraat Mühendisliği IX. Teknik Kongresi, Ankara, Türkiye.
• Audu, M. A., Falusi, O. A., Muhammad, M. L., Daudu, O. A., & Abubakar, A. (2021). Pollen Viability and Germinability of Gamma Irradiated M4 Lines of Sesame. Badeggi Jo-urnal of Agricultural Research and Environment, 03(02), 11-18. https://doi.org10.35849/BJARE202102002
• Bajaj, Y. P. & Gill, M. S. (1997). In vitro induction of haploidy in cotton. In: S. M. Jain, S. K. Sopory, & R. E. Veilleux. (Eds.), In Vitro Haploid Production in Higher Plants 5 (p-p. 165-174). Springer Science & Business Media. https://doi.org/10.1007/978-94-017-1856-1
• Bidney, D. L., & Scelonge, C. J. (1997). Sunflower biotechno-logy. In Albert A. Schneiter. (Eds.), Sunflower Techno-logy and Production 35 (pp 559-593). Agron. Monogr. 35. ASA, CSSA and SSSA, Madison, USA. https://doi.org/10.2134/agronmonogr35.c11
• Blakeslee, A. F., Belling, J., Farnham, M. E., & Bergner, A. D. (1922). A haploid mutant in the jimson weed," Datura stramonium". Science, 55(1433), 646-647. https://doi.org/10.1126/science.55.1433.64
• Blasco, M., Badenes, M. L., & del Mar Naval, M. (2016). Induced parthenogenesis by gamma-irradiated pollen in loquat for haploid production. Breeding Science, 66 (4), 606-612. https://doi.org/10.1270/jsbbs.16021
• Bohanec, B. (2003). Ploidy determination using flow cyto-metry. In: M., Maluszynski, K.J., Kasha, B.P., Forster & I. Szarejko, (Eds.), Doubled Haploid Production in Crop Plants (pp. 397-403). Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_52
• Chahal, G. S., & Gosal, S. S. (2002). Principles and procedures of plant breeding: biotechnological and conventional approaches. Alpha Science Int'l Ltd, CRC Press.
• Dal, B., Sari, N., & Solmaz, I. (2016). Effect of different irra-diation sources and doses on haploid embryoinduction in Altinbas (Cucumis melo L. var. inodorus) melons. Turkish Journal of Agriculture and Forestry, 40(4), 552-559. https://doi.org/10.3906/tar-1511-27
• Demirel, D. (1995). Flow sitometrik DNA analizinin temel prensipleri. Türk Patoloji Dergisi, 11(2), 64-65.
• Demirel, F., Eren, B., Demirel, S. & Erol, A. (2020). Flow Sitometri ve Bitki Islahı. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 34(1), 213-223.
• Dwivedi, S. L., Britt, A. B., Tripathi, L., Sharma, S., Upadhyaya, H. D., & Ortiz, R. (2015). Haploids: constraints and opportunities in plant breeding. Biotechnology advances, 33(6), 812-829. https://doi.org/10.1016/j.biotechadv.2015.07.001
• Fletcher, R., Coventry, J., & Kott, L. S. (1998). Doubled haploid technology for spring and winter Brassica napus (revi-sed ed.). Technical Bulletin p. OAC Publication, Univer-sity of Guelph, Ontario, Canada
• Friederich, J. (2020). Doubled haploid breeding methods in maize and soybean. [Doctoral dissertation]. Lowa State University.
• Germana, M. A. (2011). Anther culture for haploid and doub-led haploid production. Plant Cell, Tissue and Organ Cul-ture (PCTOC), 104, 283-300. https://doi: 10.1007/s11240-010-9852-z
• Giovannini, A., Macovei, A., Caser, M., Mansuino, A., Ghione, G. G., Savona, M., Carbonera, D., Scariot, V., & Balest-razzi, A. (2017). Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars. Plants (Basel, Switzerland), 6(2), 17. https://doi.org/10.3390/plants6020017
• Gonzalo, M. J., Claveria, E., Monforte, A. J., & Dolcet-Sanjuan, R. (2011). Parthenogenic haploids in melon: generation and molecular characterization of a doubled haploid li-ne population. Journal of the American Society for Hor-ticultural Science, 136(2), 145-154. https://doi.org/10.21273/JASHS.136.2.145
• Grouh, M. S. H., Sousaraei, N., Akbari, M., Rahimi, V., & Bayat, H. (2015). Induction of haploid plants in iris (Iris pseudacorus) by pollen irradiation. Turkish Journal of Agriculture and Forestry, 39(4), 596-600. https://doi.org/10.3906/tar-1407-18
• Hayati, P. D., Wıtarı, S., Rozen, N., Sutoyo, S., & Wıdıarsıh, S. (2022). Effect of gamma irradiation on the germina-tion, pollen viability, and morpho-agronomic of Pachyrhizus erosus cv. Kota Padang. Biodiversitas Jour-nal of Biological Diversity, 23(3), 1231-1238. https://doi.org/10.13057/biodiv/d230306
• Kaya, Y. (2004). Ayçiçeği Biyoteknolojisinde son gelişmeler ve ıslahında kullanım olanakları. Trakya Univ J Sci, 5(2),141-147. Retrieved from https://dergipark.org.tr/en/pub/trakyafbd/issue/23037/246275
• Korkunç, M., Bardak, A., Ekinci, R., & Haliloğlu, K. (2017). Farklı pamuk çeşitlerinde anter kültürü tekniğiyle kallus-tan embriyoid elde edilmesi. Dicle Üniversitesi Fen Bi-limleri Enstitüsü Dergisi, 6(3), 130-136. Retrieved from. https://dergipark.org.tr/en/download/articlenfile/991906
• Kučera, V., Vyvadilová, M., & Klíma, M. (2002). Utilisation of Doubled Haploids in Winter Oilseed Rape (Brassica na-pus L.) Breeding. Czech J. Genet. Plant Breed. 38(1), 50-54. Retrieved from. https://www.agriculturejournals.cz/pdfs/cjg/2002/01/06.pdf
• Kundu, M., Dubey, A., & Malik, S. (2016). Effect of gamma ray irradiation doses on pollen viability and in vitro germi-nation in Citrus. Indian J Agr Sci, 86(10), 106-109.
• Kundu, M., Dubey, A., Srivastav, M., Malik, S., & Singh, B. (2014). Effect of gamma ray irradiation and cryopreser-vation on pollen stainability, in vitro germination, and fruit set in Citrus. Turkish Journal of Biology, 38(1), 1-9. https://doi.org/10.3906/biy-1303-55
• Kurtar, E. S., Seymen, M., Çetin, A. N., & Türkmen, Ö. (2020). Dihaploidization in Promising Summer Squash Genoty-pes (Cucurbita pepo L.) via Irradiated Pollen Technique. Yuzuncu Yıl University Journal of Agricultural Sciences, 31(1), 42-51. https://doi.org/10.29133/yyutbd.800475
• Lerouge, S., & Simmons, A. (Eds.). (2012).Sterilisation of biomaterials and medical devices. Elsevier.
• Ludovici, G. M., Souza, S. O., Chierici, A., Cascone, M. G., d'Errico, F., & Malizia, A. (2020). Adaptation to ionizing radiation of higher plants: From environmental radioac-tivity to chernobyl disaster. Journal of Environmental Radioactivity, 222,106375. https://doi: 10.1016/j.apradiso.2019.109030
• Lulsdorf, M. M., Croser, J. S., & Ochatt, S. (2011). Androgene-sis and Doubled-Haploid Production. In Food Legumes. P. Aditya, & K. Jitendra (Eds.), Biology and Breeding of Food Legumes (pp 159-177). Wallingford UK: CABI. https://doi: 10.1079/9781845937669.0159
• Musial, K. & Przywara L. (1998) Influence of irradiated pollen on embryo and endosperm development in kiwifruit. Annals of Botany, 82(6), 747-756. https://doi.org/10.1006/anbo.1998.0747
• Özer, S. (2016). Bazı Yabani Ayçiçeği Türlerinin (Helianthus Spp.) Morfolojik ve Fenolojik Karakterizasyonu ve Türler Arası Melez Performanslarının in vıtro ve in vıvo Koşul-larda Araştırılması. [Yayımlanmamış Doktora Tezi]. Uludağ Üniversitesi.
• Pérez-Jiménez, M., Tallón, C. I., & Pérez-Tornero, O. (2020). Inducing mutations in Citrus spp.: Sensitivity of diffe-rent sources of plant material to gamma radiation. Applied Radiation and Isotopes, 157, 109030. https://doi: 10.1016/j.apradiso.2019.109030
• Puolimatka, M., & Pauk, J. (2000). Effect of induction duration and medium composition on plant regeneration in wheat (Triticum aestivum L.) anther culture. Journal of plant physiology, 156(2), 197-203. https://doi.org/10.1016/S0176-1617(00)80306-5
• Riviello-Flores, M. D. L. L., Cadena-Iñiguez, J., Ruiz-Posadas, L. D. M., Arévalo-Galarza, M. D. L., Castillo-Juárez, I., Soto Hernández, M., & Castillo-Martínez, C. R. (2022). Use of gamma radiation for the genetic improvement of underutilized plant varieties. Plants, 11(9), 1161. https://doi: 10.1016/j.apradiso.2019.109030
• Ryu, J. H., Doo, H. S., & Kwon, T. H. (1992). Induction of hap-loid plants by anther culture in sesame (Sesamum indi-cum L.)-(1)-effects of growth regulators and difference between genotypes on callus induction. Korean Journal of Plant Tissue Culture (Korea Republic), 19(3), 171-177.
• Sakiroglu, M., & Kaya, M. M. (2012). Estimating genome size and confirming ploidy levels of wild tetraploid alfalfa accessions (Medicago sativa subsp.× varia) using flow cytometry. Turkish Journal of Field Crops, 17(2), 151-156.
• Sestili, S., & Ficcadenti, N. (1996). Irradiated pollen for hap-loid production. In Vitro Haploid Production in Higher Plants, 263-274. https://doi:10.1007/978-94-017-1860-8_15
• Seyis, F., Aydın, E., & Çatal, M. İ. (2014). Haploids in the improvement of Crucifers. Turkish Journal of Agricultu-ral and Natural Sciences, 1(2), 1419-1424. Retrieved from https://dergipark.org.tr/en/pub/turkjans/issue/13311/160923
• Shahhosseini, G. (2022). Evaluation of Growth and Immuno-logical Indices of Allfemale Caspian salmon Salmo trut-ta caspius Induced by Gamma Irradiation. Brazilian Archives of Biology and Technology, 64. https://doi.org/10.1590/1678-4324-2021200752
• Shu, Q. Y., Forster, B. P., Nakagawa, H., & Nakagawa, H. (Eds.). (2012). Plant mutation breeding and biotechno-logy. Cabi.
• Shuryak, I., Tkavc, R., Matrosova, V. Y., Volpe, R. P., Grichen-ko, O., Klimenkova, P. Conze, I. H., Balygina, I. A., Gai-damakova, E. K., & Daly, M. J. (2019). Chronic gamma radiation resistance in fungi correlates with resistance to chromium and elevated temperatures, but not with resistance to acute irradiation. Scientific reports, 9(1), 11361. https://doi: 10.1038/s41598-019-47007-9
• Spencer-Lopes, M. M., Forster, B. P., & Jankuloski, L. (2018). Manual on mutation breeding (No. Ed. 3). Food and Agriculture Organization of the United Nations (FAO).
• Stanley, R. G. & Linskens, H. F. (1974). Pollen: Biology Bioc-hemistry and Management. Springer, Berlin. https://doi.org/10.1007/978-3-642-65905-8
• Sulusoglu, M., & Cavusoglu, A. (2014). In vitro pollen viability and pollen germination in cherry laurel (Prunus lauroce-rasus L.). The Scientific World Journal, 2014,1-7. https://doi.org/10.1155/2014/657123
• Şanver, P., & Göksoy, A. T. (2019). Hibrid ayçiçeği genotiple-rinde korelasyon ve path analizi. Journal of Agricultural Faculty of Bursa Uludag University, 33(2), 235-248. Ret-rieved from. https://dergipark.org.tr/en/pub/bursauludagziraat/issue/50138/554361
• Todorova, M., Ivanov, P., Shindrova, P., Christov, M., & Ivano-va, I. (1997). Doubled haploid production of sunflower (Helianthus annuus L.) through irradiated pollen-induced parthenogenesis. Euphytica, 97(3), 249-254. https://doi.org/10.1023/A:1002966824988
• Wanner, G., Formanek, H., Martin, R., & Herrmann, R. G. (1991). High resolution scanning electron microscopy of plant chromosomes. Chromosoma, 100(2), 103-109.
• Yaman, H. (2014). Aspir (Carthamus tinctorius L.) Çeşitlerine Uygulanan Farklı Gama Işını Dozlarının M1 ve M2 Bitki-lerinin Bazı Tarımsal Özellikleri ve In Vitro Adventif Sür-gün Rejenerasyonu Üzerine Etkileri [Yayımlanmamış Doktora Tezi]. Ankara Üniversitesi.
• Yorgancılar, M., Yaşar, M. A., & Atalay, E. (2019). Mısır Isla-hında İndirgeyici Hatların Kullanımı ve Dihaploidizasyon. Journal of Bahri Dagdas Crop Research, 8(1), 170-177. from. https://dergipark.org.tr/en/pub/bdbad/issue/47287/596070
• Zhao, Q., Zhang, M., Qi, H., & Yang, X. (2022). Haploid induc-tion and plant production in bottle gourd by pollination with gamma irradiated pollen. Plant Cell, Tissue and Organ Culture (PCTOC), 1-8. https://doi.org/10.21203/rs.3.rs-2019755/v1
• Zheng, M., Liu, W., Weng, Y., Polle, E., & Konzak C. (2001). Culture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals. Plant Cell Rep, 20(8), 685-690. https://doi.org/10.1007/s00299-001-0393-0