Isolation and identification of entomopathogenic fungi from coastal districts of Ordu province, Turkey

Abstract

A total of 250 soil samples were taken from the forest, hazelnut, kiwi, vegetable, and meadow-rangeland areas in the coastal regions of Ordu province, Turkey. Entomopathogenic fungi were isolated from these soil samples using the Galleria-bait method. Eighty-five fungal isolates were isolated from these soil samples, after which they were morphologically and molecularly identified. After morphological characterization, 64 out of 85 isolates were identified molecularly. Based on the molecular characterization results, twenty-three out of the 64 isolates were Beauveria bassiana (35.94%), 11 isolates were Metarhizium brunneum (17.19%), 8 isolates were Metarhizium anisopliae (12.5%), 6 isolates were Metarhizium robertsii (9.38%), 4 isolates were Purpureocillium lilacinum (6.25%), 4 isolates were Clonostachys rogersoniana (6.25%), 3 isolates were Fusarium solani (4.69%), 1 isolate was Clonostachys rossmaniae (1.56%), 1 isolate was Aspergillus flavus (1.56%), 1 isolate was Cordyceps cicadae (1.56%), 1 isolate was Cordyceps fumosorosea (1.56%), and 1 isolate was Fusarium oxysporum (1.56%). In the coastal area of Ordu province, the most common entomopathogen fungal genus is Metarhizium followed by Beauveria bassiana.

Keywords

• entomopathogenic fungi
• isolation
• biological control
• forest
• hazelnut
• Black Sea

Ordu ili kıyı ilçelerinden entomopatojen fungusların izolasyonu ve tanımlanması

Abstract

Ordu ili kıyı bölgelerindeki orman, fındık, kivi, sebze ve çayır-mera alanlarından toplam 250 adet toprak örneği alınmıştır. Bu toprak örneklerinden Galleria-tuzak yöntemi kullanılarak entomopatojen funguslar izole edilmiştir. İzolasyonlar sonucunda 85 fungal izolat elde edilmiştir. Morfolojik karakterizasyondan sonra 85 izolattan 64'ü moleküler olarak tanımlanmıştır. Moleküler karakterizasyon sonuçlarına göre 64 izolattan 23'ü Beauveria bassiana (%35.94), 11 izolat Metarhizium brunneum (%17.19), 8 izolat Metarhizium anisopliae (%12.5), 6 izolat Metarhizium robertsii (%9.38), 4 izolat Purpureocillium lilacinum (%6.25), 4 izolat Clonostachys rogersoniana (%6.25), 3 izolat Fusarium solani (%4.69), 1 izolat Clonostachys rossmaniae (%1.56), 1 izolat Aspergillus flavus (%1.56), 1 izolat Cordyceps cicadae (%1.56), 1 izolat Cordyceps fumosorosea (%1.56) ve 1 izolat Fusarium oxysporum (%1.56) idi. Ordu ilinin kıyı kesimlerinde en yaygın entomopatojen mantar türü Metarhizium'dur ve bunu Beauveria bassiana izlemektedir.

Keywords

• entomopatojen fungus
• izolasyon
• biyolojik mücadele
• orman
• fındık
• Karadeniz

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