qPCR analysis of Puccinia spp. in turfgrass areas in Türkiye

Abstract

In recent years, with the increase in parks, gardens, recreation areas, picnic areas and refuges with urbanization, the interest in turfgrass areas that beautify the aesthetic appearance of these areas has also increased. In these areas, rust diseases are also becoming increasingly common and causing problems. Rust diseases are an important pathogen group that needs to be monitored due to their ability to create new races and their airborne transmission. In this study, surveys were conducted in turfgrass areas in 8 provinces of Türkiye and 110 turfgrass leaf samples showing rust symptoms were collected. As a result of DNA isolation and qPCR analysis from pustules on the leaf surface, 37 Puccinia coronata, 32 P. graminis, 18 P. striiformis and 23 Puccinia spp. have been detected. It was determined that the most common rust species that causes disease in turfgrass areas in Türkiye is P. coronata. This species was followed by P. graminis and P. striiformis, respectively. While P. striiformis was mostly isolated from Kayseri and Istanbul provinces, P. coronata and P. graminis were mostly isolated from İstanbul.

Keywords

• turfgrass
• rust
• real time PCR
• Türkiye

Türkiye'deki çim alanlarında Puccinia spp.nin qPCR analizi

Öz

Son yıllarda şehirleşmeyle birlikte park, bahçe, rekreasyon alanları, piknik alanları ve refüjlerin artmasıyla birlikte bu alanların estetik görünümünü güzelleştiren çim alanlarına ilgi de artmıştır. Bu alanlarda pas hastalıkları da giderek yaygınlaşmakta ve sorun oluşturmaktadır. Pas hastalıkları, yeni ırk oluşturma kapasiteleri ve hava yoluyla taşınmaları sebebiyle takip edilmesi gereken önemli bir patojen grubudur. Bu çalışmada, Türkiye'nin 8 ilindeki çim alanlarında sürveyler düzenlenerek, 110 adet pas belirtisi gösteren çim yaprak örneği toplanmıştır. Yaprak yüzeyindeki püstüllerden DNA izolasyonu ve qPCR analizi sonucunda 37 adet Puccinia coronata, 32 adet P. graminis, 18 adet P. striiformis ve 23 adet Puccinia spp. tespit edilmiştir. Türkiye'de çim alanlarında hastalığa neden olan en yaygın pas türünün P. coronata olduğu saptanmıştır. Bu türü sırasıyla P. graminis ve P. striiformis takip etmiştir. P. striiformis en fazla Kayseri ve İstanbul ilinden izole edilirken, P. coronata ve P. graminis en fazla İstanbul ilinden izole edilmiştir.

Anahtar Kelimeler

• çim
• pas
• real time PCR
• Türkiye

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