Investigation of the effect of Beauveria bassiana (Balsamo) Vuillemin against potato beetle (Leptinotarsa decemlineata Say.) (Coleoptera: Chrysomelidae)

Abstract

In this study, the objective was to determine the efficacy of entomopathogenic fungi on the Colorado potato beetle [Leptinotarsa decemlineata Say. (Coleoptera: Chrysomelidae)]. To reach this goal, surveys were conducted in potato fields, and dead L. decemlineata and soil samples were collected to isolate entomopathogenic fungi. Pathogenicity tests were carried out using 30 entomopathogenic fungi that were obtained as a result of the analyses. According to the biological activity results, EP-1 isolate was identified and diagnosed as having 75% efficacy against L. decemlineata. Beauveria bassiana (EP-1), was identified as the most effective isolate. In the dose determination studies, 106, 107, and 108 conidia ml/l doses of B. bassiana's most effective isolate were used. The experiments were set up with five replications for each dose and control group. The spore suspension of the entomopathogenic fungus was sprayed on fully-grown potato plants cultivated in pots, targeting mature and 2nd or 3rd instar L. decemlineata. The number of live individuals was recorded on the 1st, 3rd, 5th, 7th, and 9th days after application to calculate the percentage of mortality. The most effective dose of B. bassiana isolate was determined to be 85% mortality on the 7th day after application with a dose of 108 conidia ml/l. According to the obtained data, it was observed that the mortality rates increased with the increase in dose on the 1st, 3rd, 5th, 7th, and 9th days after application. The highest impact was observed in applications with a dose of 108 conidia ml/l. In conclusion, the entomopathogenic fungus B. bassiana isolate, which is less harmful to humans and the environment, is considered suitable for use as a biological control agent against L. decemlineata.

Keywords

• potato
• potato beetle
• biological control
• mortality

Beauveria bassiana (Balsamo) Vuillemin'in Patates böceği (Leptinotarsa decemlineata Say.) (Coleoptera: Chrysomelidae)'ne karşı etkisinin araştırılması

Abstract

Entomopatojen fungusların Patates böceği (Leptinotarsa decemlineata) üzerindeki etkinliğini belirlemek amacıyla bu çalışma yapılmıştır. Bu amaçla patates ekim alanlarında sürveyler yapılarak entomopatojen fungusların izolasyonu için ölü patates böcekleri ile toprak örnekleri toplanmıştır. Yapılan analizler sonucunda elde edilen 30 adet entomopatojen fungus ile patajonisite testleri gerçekleştirilmiştir. Biyolojik etkinlik sonuçlarına göre L. decemlineata’ya karşı %75 etki oranında EP-1 izolatı belirlenmiş ve tanısı yapılmıştır. Beauveria bassiana’nın (EP-1) en etkili izolat olduğu belirlenmiştir. Doz belirleme çalışmalarında ise, B. bassiana izolatından 106, 107 ve 108 konidi ml/l dozları kullanılmıştır. Yapılan çalışmalarda, her doz ve kontrol grubu için beş tekrarlı deneme kurulmuştur. Kontrollü koşullarda saksılarda yetiştirilen patates bitkileri üzerindeki ergin, 2. ve 3. dönem Patates böceği larvalarına entomopatojen fungusların spor süspansiyonu püskürtülmüştür. Canlı bireylerin sayısı, uygulamadan sonraki 1., 3., 5., 7. ve 9. günlerde kaydedilmiş ve ölüm yüzdesini hesaplamak için kullanılmış, B. bassiana izolatının en etkili dozu, 108 konidi ml/l dozuyla uygulamanın 7. gününde %85 ölüm olarak belirlenmiştir. Elde edilen verilere göre, ölüm oranlarının dozun artmasıyla 1., 3., 5., 7. ve 9. günlerde arttığı gözlemlenmiştir. En yüksek etki, 108 konidi ml/l dozuyla yapılan uygulamalarda gözlenmiştir. Sonuç olarak, insanlar ve çevre için daha az zararlı olan entomopatojen fungus izolatı B. bassiana’nın, L. decemlineata'ya karşı biyolojik kontrol ajanı olarak kullanılması uygun görülmektedir.

Keywords

• patates
• patates böceği
• biyolojik kontrol
• ölüm oranı

References

1. Alisdair R., Fernie R., Willmitzer L., 2001. Update on tuber formation, dormancy and sprouting molecular and biochemical triggers of potato tuber development. Plant Physiology, 127, 1459-1465.
2. Behie S.W., Zelisko M.P., Bidochka M.J., 2012. Endophytic insect-parasitic fungi translocate nitrogen directly from insects to plants. Science, 336, 1576–1577. 10.1126/science.1222289
3. Boucias D.G., Pendland J.C., 1998. Entomopathogenic fungi: fungi imperfecti. In: Principles of Insect Pathology. Boucias, D.G., Pendland, J. C., (Eds.). Klewer Academic Publisher, Boston, 321-364 p.
4. Butt T.M., Ibrahim L., Ball B.V., Clark S.J., 1994. Pathogenicity of the entomogenous fungi Metarhizium anisopliae and Beauveria bassiana against crucifer pests and the honey bee. Biocontrol Science and Technology, 4 (2), 207-214, doi:10.1080/09583159409355328
5. Calbrix R., Laval K., Barray S., 2005. Analysis of the potential functional diversity of the bacterial community in soil: a reproducible procedure using sole-carbon-source utilization profiles. European Journal of Soil Biology, 41 (1), 11-20. doi:10.1016/j.ejsobi.2005.02.004
6. Çam H., Gökçe A., Yanar Y., Kadıoğlu İ., 2002. Entomopatojen fungus Beauveria bassiana (Bals.) Vuill.'nın patates böceği, Leptinotarsa decemlineata (Say.) üzerindeki etkisi. 359-364. Türkiye 5. Biyolojik Mücadele Kongresi, 4-7 Eylül 2002, Erzurum, Türkiye, 483 s.
7. Erdoğan P., 2015. Insecticidal effects of extracts of Capsicum annuum L. (Solanaceae and Allium sativum L. (Amaryllidaceae) on Myzus persicae (Sulzer) [(Hemiptera: Aphididae)]. Plant Protection Bulletin (Bitki Koruma Bülteni), 55 (4), 305-315.
8. Fernandez S., Groden E., Vandenberg J.D., Furlong M.J., 2001. The effect of mode of exposure to Beauveria bassiana on conidia acquisition and host mortality of Colorado potato beetle, Leptinotarsa decemlineata. Journal of Invertebrate Pathology, 77 (3), 217-26. doi: 10.1006/jipa.2001.5022.

9. Kia S.H., Glynou K., Nau T., Thines M., Piepenbring M., Macia-Vicente J.G., 2017. Influence of phylogenetic conservatism and trait convergence on the interactions between fungal root endophytes and plants. ISME Journal, 11 (3), 777-790. doi: 10.1038/ismej.2016.140.
10. McKinnon A.C., Glare T.R., Ridgway H.J., Mendoza-Mendoza A., Holyoake A., Godsoe W.K., Bufford J.L., 2018. Detection of the entomopathogenic fungus Beauveria bassiana in the rhizosphere of wound-stressed Zea mays plants. Frontiers in Microbiology, 9, 1161. doi: 10.3389/fmicb.2018.01161
11. Meyling N.V., Eilenberg J., 2007. Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: potential for conservation biological control. Biological Control, 43, 145–155. 10.1016/j.biocontro1.2007.07.007
12. Oerke E.C., Dehne H.W., Schonbeck F., Weber A., 1994. Crop production and crop protection: estimated losses in mojor food and cash crops. Elsevier, Amsterdam, Netherlands, 808 pp.
13. Rath A.C., 2000. The use of entomopathogenic fungi for control of termites. Biocontrol Science and Technology, 10, 563-581. https://doi.org/10.1080/095831500750016370
14. Saruhan I., Erper I., Tuncer C., Akca I., 2015. Efficiency of some entomopathogenic fungi as biocontrol agents against Aphis fabae Scopoli (Hemiptera: Aphididae). Pakistan Journal Agriculture Science, 52 (2), 273–278.
15. Todorova S.I., Coderre D., Côté J.C., 2000. Pathogenicity of Beauveria bassiana isolates toward Leptinotarsa decemlineata [Coleoptera: Chrysomelidae], Myzus persicae [Homoptera: Aphididae] and their predator Coleomegilla maculata lengi [Coleoptera: Coccinellidae]. Phytoprotection, 81 (1), 15-22.
16. TUİK, 2022. Ülkemizdeki patates üretim alanları. www.tuik.gov.tr (accessed date: 05.02.2023).
17. Uysal H.M., Demirözer O., Uzun Yiğit A., 2022. Determination of lethal effects of some entomopathogenic fungi on different biological stages of Leptinotarsa decemlineata Say. KSU Journal of Agriculture and Nature, 25 (5), 1036-1041. https://doi.org/10.18016/ksutarimdoga.vi.928366
18. Uzuner S., Güner B.G., Ayar Ö., Yaman M., 2017. The effects of entomopathogenes used in biological control on bees. Bee Studies, 9 (1), 9-19.
19. Vidal S., Jaber L.R., 2015. Entomopathogenic fungi as endophytes: plant-endophyte-herbivore interactions and prospects for use in biological control. Current Science, 109, 46–54.
20. Wraight S.P., Ramos M.E., 2015. Delayed efficacy of Beauveria bassiana foliar spray applications against Colorado potato beetle: impacts of number and timing of applications on larval and next generation adult populations. Biological Control, 83, 51-67. https://doi.org/10.10 16/j.
21. Yüceer S., 2011. Patates Böceği (Leptinotarsa decemlineata Say.)’ne Dayanıklı Bitkiler Elde Etmek Amacıyla Patates (Solanum tuberosum L.)’in Genetik Transformasyonu, Doktora Tez Projesi, Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Adana, 134 s.
22. Zimmermann G., 1986. The ‘Galleria bait method’ for detection of entomopathogenic fungi in soil. Journal of Applied Entomology, 102 (1-5), 213-215.
23. Zimmermann G., 2007. Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Science and Technology, 17 (5/6), 553-596. https://doi.org/10.1080/09583150701309006F