İstilacı Xylosandrus compactus'un (Coleoptera: Curculionidae: Scolytinae) moleküler ve morfolojik tanısı ve Türkiye için yeni konukçu bitki kayıtları

Abstract

Polifag istilacı zararlı Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae: Scolytinae) yakın zamanda Türkiye'de bildirilmiştir. Bu tür, istila ettiği ve yerleştiği topraklarda odunsu süs ve tarım bitkileri ile orman ağaçları için ekonomik kayıplara neden olur. Bu zararlının oldukça yüksek bir yayılma oranına sahip olduğu ve kontrolünün doğru tanımlamaya dayandığı göz önünde tutulmalıdır. Bu tür, mevcut çalışmada sağlanan morfolojik ve moleküler özellikler kullanılarak diğer ambrosya böceklerinden ayırt edilebilir. Türkiye'de toplanan bazı örneklerden alınan mtDNA gen bölgesi, moleküler tanımlama için kullanılmış ve NCBI veri tabanlarında hali hazırda mevcut olan "barkod" dizileriyle karşılaştırılmıştır. Sekiz farklı konukçu bitki türünden elde edilen X. compactus örneklerinden alınan COI dizileri filogenetik analizde kullanılmıştır. Türkiye'deki bu biyolojik istilanın kökenini anlamak için bu ambrosia böceğinin doğal bulunduğu ve sonradan girdiği alanların genetik çeşitliliği hakkında daha fazla araştırma yapılması gerekmektedir. Türkiye'de tespit edilmesinden bu yana X. compactus 32 konukçu bitki türünde belirlenmiş olup, bu çalışmada sekiz yeni konukçu bitki türünün tanımlanmasıyla bu sayı 40’a çıkmıştır. X. compactus için Dünya konukçu tür listesine Hydrangea macrophylla (Thunb.) Ser. ve Fraxinus ornus L. hariç altı yeni tür eklenmiştir.

Keywords

• ambrosiya böcekleri
• moleküler tanılama
• COI sekansı
• konukçu bitki
• istilacı türler

Molecular and morphological identification of the invading Xylosandrus compactus (Coleoptera: Curculionidae: Scolytinae) and novel host plant records for Türkiye

Abstract

The polyphagous invasive pest Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae: Scolytinae) has recently been reported in Türkiye. This species causes economic losses for woody ornamental and agricultural plants and forest trees in the lands where it invaded and settled. It should be considered that this pest has a pretty high rate of spread, and its control relies on accurate identification. This species could be distinguished from other ambrosia beetles using both morphological and molecular characteristics provided by the present study. The mtDNA gene region from some specimens collected in Türkiye was used for molecular identification and compared to "barcode" sequences already present in NCBI databases. COI sequences from X. compactus individuals obtained from eight distinct host plant species were used in phylogenetic analysis. More research into the genetic diversity of this ambrosia beetle's native and introduced areas is required to understand the origin of this biological invasion in Türkiye. Since its definition in Türkiye, X. compactus has been detected in 32 host plant species, and this study has increased that number to 40 by identifying eight new host plant species. Except Hydrangea macrophylla (Thunb.) Ser. and Fraxinus ornus L., six new species have been added to the world host plant species list for X. compactus.

Keywords

• ambrosia beetles
• molecular identification
• COI sequence
• host plant
• invasive species

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