Salvia officinalis L. (Lamiaceae) uçucu yağının Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) ve Rhyzopertha dominica Fabricius (Coleoptera: Bostrichidae)’ya karşı davranışsal ve insektisidal aktivitesi

Öz

Salvia türlerinin depolanmış ürün zararlısı böcekler üzerindeki insektisidal ve davranışsal etkileri uzun süreden beri bilinmektedir. Bu çalışmanın amacı, Salvia officinalis L. (Lamiaceae) bitkisinden elde edilen uçucu yağın iki önemli depolanmış ürün zararlısı olan Tribolium confusum Jacquelin du Val (Col: Tenebrionidae) ve Rhyzopertha dominica (Fabricius) (Col: Bostrichidae)’ya karşı repellent ve kontakt toksisitesini laboratuvar koşullarında test etmektir. Bu amaçla repellent aktivite testlerinde dört konsantrasyon (0.047 µl/cm², 0.118 µl/cm², 0.236 µl/cm² ve 0.590 µl/cm²) denemeye alınmış ve denemeler cam petrilerde yürütülmüştür. Kontakt aktivite testlerinde ise %10 (v/v) konsantrasyonda hazırlanan uçucu yağ çözeltileri mikroaplikatör yardımı ile 0.1 µl/böcek dozunda uygulanmış ve 24 saat sonunda ölü bireyler kaydedilmiştir. Rhyzopertha dominica’ya karşı en yüksek repellent etki 1/2 saat sonunda 0.118 µl/cm² konsantrasyonda %53.33 olarak tespit edilmiş, benzer şekilde T. confusum’da 0.118 µl/cm² konsantrasyonda 1/2 saat sonunda %36.67’lik bir etki belirlenmiştir. Kontakt toksisite çalışmalarında R. dominica için %91.02 ölüm oranı belirlenirken T. confusum için bu etki %33.67 olmuştur. Çalışma sonucunda S. officinalis uçucu yağının R. dominica'nın ergin bireyleri üzerinde önemli bir kontak toksisiteye sahip olduğu belirlenmiş olup ileride yapılacak çalışmalar açısından ümitvar olduğu kanısına varılmıştır.

Anahtar Kelimeler

• repellent,kontakt aktivite,Lamiaceae,Tribolium confusum,uçucu yağ,Rhyzopertha dominica

Behavioural and insecticidal activity of Salvia officinalis L. (Lamiaceae) essential oil against Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) and Rhyzopertha dominica Fabricius (Coleoptera: Bostrichidae)

Abstract

The insecticidal and behavioural effects of Salvia spp. on stored product pests has been known for a long period. The aim of this work is to investigate the efficacy of essential oil obtained from sage [Salvia officinalis L. (Lamiaceae)] as repellent and contact toxicity against two important stored grain insects Tribolium confusum Jacquelin du Val (Col: Tenebrionidae) and Rhyzopertha dominica (Fabricius) (Col: Bostrichidae) under laboratory conditions. In the repellent activity test, four concentrations were used (0.047 µl/cm², 0.118 µl/cm², 0.236 µl/cm² and 0.590 µl/cm²) and the experiments were carried out on glass petri dishes. In contact activity tests, essential oil solutions prepared at a concentration of 10% (v/v) were applied to insects with a hand-operated microapplicator at 0.1 µl/insect dose and dead individuals were recorded after 24 hours. The highest repellent activity was seen against R. dominica at 53.33%, at 0.118 µl/cm² concentration after half an hour, compared with a rate of 36.67% at 1/2 hour of 0.118 µl/cm² dose in T. confusum. In contact toxicity studies, 91.02% mortality was found for R. dominica, while for T. confusum this mortality was 33.67%. The results of this study show that essential oils of S. officinalis have significant contact toxicity on adults of R. dominica.

Keywords

• repellent,Lamiaceae,Tribolium confusum,essential oil,contact activity,Rhyzopertha dominica

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