Survey for Grapevine leafroll viruses (GLRaVs) in Malatya region

Abstract

Grapevine (Vitis vinifera L.) is one of the most important fruit crops in Turkey and viral diseases are one of the main problems of grapevine production. They decrease yield quantity and quality of crops as well as cause death of the plants in an interaction with other agents. Leafroll disease of grapevine, caused by a complex of viruses, is considered a serious threat to production across many grapevine growing regions in Turkey. This study was conducted to determine the current status of Grapevine leafroll-associated viruses (GLRaVs) in Malatya province. Grapevine plantations in Malatya province were surveyed for the presence of Grapevine leafroll-associated viruses (GLRaVs) -1, -2, -3, -4 and -6. Purposive sampling was done in vineyards of Arapgir, Darende and Battalgazi districts, which are the primary grapevine producing areas of Malatya during 2016-2017 growing seasons. A total of 241 samples were collected and tested by DAS-ELISA. Samples giving two fold of absorbance values (405 nm) of negative control were evaluated as positive. According to the results, 131 samples were found to be infected with GLRaV-1, whereas 48 samples with GLRaV-3 and -4 samples with GLRaV-4. None of the samples were reacted as positive against GLRaV-2 and GLRaV-6. Forty five samples were found to be infected with mix infection of GLRaV-1 and -3, and three samples were found to be infected with GLRaV-1 and -4 strain 6.

Keywords

• Survey
• grapevine
• Grapevine leafroll associated viruses
• Malatya

Malatya bölgesi bağlarında Asma yaprak kıvrılma virüsleri (GLRaVs)’nin sürveyi

Abstract

Asma (Vitis vinifera L.) ülkemizde yetiştiriciliği yapılan en önemli meyvelerden birisi olup üretimini sınırlayan faktörlerin başında virüs hastalıklar gelmektedir. Virüs hastalıkları üzüm üretimini kalite ve kantite bakımından azaltmanın yanı sıra diğer hastalık etmenleri ile beraber bulunmaları durumunda asmanın kurumasına kadar ulaşabilen ciddi sonuçlar doğurabilmektedir. Bir virüs kompleksinin neden olduğu Asma yaprak kıvrılma hastalığı ülkemiz asma yetiştiriciliği üzerinde önemli bir tehdittir. Bu çalışma, Malatya ilinde yetiştirilen asmaları etkileyen Asma yaprak kıvırcıklık virüsleri (Grapevine leafroll associated virus, GLRaV)’ini tespit etmek amacı ile yürütülmüştür. Bu amaçla Malatya ilindeki asma bahçeleri gezilerek etmenin 1, 2, 3, 4 ve 6 nolu ırkları araştırılmıştır. 2016-2017 yıllarında Arapgir, Darende ve Battalgazi ilçelerini kapsayan survey çalışmalarında güdümlü örneklemeler yapılarak asma numuneleri toplanmıştır. Toplanan 241 örneğin tamamı DAS-ELSA metodu ile testlenmiştir. Testlerde negatif örneğin iki katı ve yukarısında absorbans (405 nm) değeri veren numuneler infekteli kabul edilmiştir. Yürütülen test sonuçlarına göre toplam 131 numunenin GLRaV 1 ile, 48 numunenin GLRaV 3 ile ve 4 örneğin ise GLRaV 4 ile infekteli olduğu tespit edilmiştir. Örneklerin hiçbirinde GLRaV 2 ve GLRaV 6 infeksiyonuna rastlanmamıştır. Testlenen 45 numunenin GLRaV 1 ve 3 ile, üç numunenin ise GLRaV 1 ve 4 ile karışık infeksiyon halinde bulunduğu tespit edilmiştir. 

Keywords

• Survey
• grapevine
• Grapevine leafroll associated viruses
• Malatya

References

1. Anonymous, 2018. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr. (Accession date: 14.03.2018).
2. Akbaş, B., Kunter, B. İlhan, D. 2009. Influence of leafroll on local grapevine cultivars in agroecological conditions of Central Anatolia region. Horticultural Science.36(3)
3. Akbaş, B., Kunter, B., Ilhan, D. 2007. Occurrence and Distribution of Grapevine Leafroll-associated Viruses 1, 2, 3 and 7 in Turkey. J. Phytopathology 155, 122–124.
4. Almeida R., Daane K., Bell V., Blaisdell G., Cooper M., Herrbach E. 2013.Ecology and management of grapevine leafroll disease. Front. Microbiol. 4:94.
5. Atallah S., Gomez M., Fuchs M., Martinson T., 2012. Economic impact of grapevine leafroll disease on Vitis vinifera cv. Cabernet franc in Finger Lakes vineyards of New York. American Journal of Enology and Viticulture 63: 73-79
6. Cabaleiro, C., Segura, A. 2006. Temporal analysis of grapevine leafroll associated virus 3 (GLRaV-3) epidemics. European Journal of Plant Pathology 114, 441-446.
7. Cabaleiro, C., Segura, A., Berrios, J.G. 1999. Effects of grapevine leafroll-associated virus 3 on the physiology and must of Vitis vinifera L. cv. Albarino following contamination in the field. American Journal of Enology and Viticulture 50(1):40-44
8. Çağlayan, K. 1997. lncidence of Grapevine Leafroll, Grapevine virus A and romato Black Ring Virus in the Vineyards of Hatay province. The Journal of Turkish Phytopath., 121-129.

9. Charles J. G., Cohen D., Walker J. T. S., Forgie S. A., Bell V. A., Breen K. C. 2006. A Review of Grapevine Leafroll-associated Virus Type 3 (GLRaV-3) for the New Zealand Wine Industry. Report to New Zealand Wine growers. Hort Research Client Report. 18447, 79.
10. Çiftçi O., Ulubaş Serçe Ç and Güler B. 2015. Survey of grapevine viruses in the east and Southeast Regions of Turkey., 18th Congress of the İnternational Council for the Study of Virus and VirusLike Diseases of the Grapevine (ICVG)., September 7-11, 2015 Ankara-Turkey., 200.
11. Clark, M.F., Adams, A.N.1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol. 34(3):475-83.
12. Erilmez, S. and Kaya, A. 2016. Asma virüslerinin tanılanmasında DAS-ELISA ve RT-PCR yöntemlerinin duyarlılıklarının karşılaştırılması. Bitki Koruma Bülteni, 56(1): 1-14.
13. Goszczynski, D. E., Kasdorf, G. G. F., Pietersen, G. 1995. Production and use of antisera specific to grapevine leafroll-associated viruses following electrophoretic separation of their proteins and transfer to nitrocellulose. Afr. Plant Prot.1, 1–8
14. Gugerli P., B. Rosciglione, J.J. Brugger, S. Bonnard, M.E. Ramel e F. Tremea. 1990. Etiological studies and diagnosis of grapevine leafroll disease improved by monoclonal antibodies. In: A. Shots (ed.) Monoclonal antibodies in Agriculture. Proceedings Symposium ìPerspectives for monoclonal antibodies in agricultureî, Wageningen 1990. Pudoc, Wageningen, 47-54.
15. Gugerli, P., Brugger, J.J. Bovey, R. 1984. Líenroulement de la vigne: mise en Èvidence de particles virales et dÈveloppement díune mÈthode immuno-enzimatique pour le diagnostic rapide. Revue Suisse de Viticulture, Arboriculture, Horticulture 16, 299-304.
16. Krake, L.R. 1993. Characterization of grapevine leafroll disease by symptomatology. The Australian and New Zealand Wine Industry Journal 8: 40-44.
17. Ling K., Zhu H., Jiang Z., Gonsalves D. 2000. Effective application of DAS-ELISA for detection of grapevine leafroll-associated closterovirus-3 using a polyclonal antiserum developed from recombinant coat protein. Eur. J. Plant Pathol. 106, 301–309.
18. Ling K., Zhu H., Petrovic N., Gonsalves D. 2001.Comparative effectiveness of ELISA and RT-PCR for detecting grapevine leafroll-associated closterovirus-3 in field samples. Am. J. Enol. Vitic.52, 21–27
19. Martelli G.P., Boudon-Padieu E., 2006. Directory of infectious diseases of grapevines. Options Méditerranéennes, Series B 55: 11-201.
20. Martelli, G.P. 1986. Virus and virus like diseases of the grapevine in the Mediterranean area. FAO Plant Prot Bull. 34:25–42.
21. Martelli, G.P. 1987. Virus and virus-like diseases of grapevine in Turkey. Rome, FAO: 32.
22. Martelli, G.P., Abou Ghanem-Sabanadzovic, N., Agranovsky, A.A. , Al Rwahnih, M., Dolja, V.V., Dovas, C.I., Fuchs, M., Gugerli, P., Hu, J.S., Jelkmann, W., Katis, N.I., Maliogka, V.I., Melzer, M.J., Menzel, W., Minafra, A., Rott, M.E., Rowhani, A., Sabanadzovic, S. and P. Saldarelli. 2012. Taxonomic revision of the family Closteroviridae with special reference to the Grapevine Leafroll Associated members of the genus Ampelovirus and the putative species unassigned to the family. Journal of Plant Pathology, 94 (1), 7-19
23. Martelli, G.P., and Boudon-Padieu, E. 2006. Directory of Infectious Diseases of Grapevines. Option Méditerraneénnes, Ser. B. 55: 7-201.
24. Martin, R.R., Eastwell, K.C., Lamprecht, S., Tzanetakis, I.E. 2005. Survey for viruses of grapevine in Oregon and Washington. Plant Dis 89:763–766.
25. Martin, R.R., Eastwell, K.C., Lamprecht, S., Tzanetakis, I.E., 2005. Survey for viruses of grapevine in Oregon and Washington. Plant Disease, 89: 763–766.
26. Naidu R., Rowhani, A., Fuchs, M., Golino, D., Martelli, G. 2014. Grapevine Leafroll: A Complex Viral Disease Affecting a High-Value Fruit Crop. Plant Disease, Vol. 98 No. 9. 1172-1185.O’Donnell, K. 1999. Plant pathogen diagnostics: present status and future developments. Potato Res.42, 437–447.
27. Pietersen, G. 2006. Spatio-temporal distribution dynamics of grapevine leafroll disease in Western Cape vineyards. Pg. 126-127. Extended abstracts of the 15th Meeting of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG) 3-7 April, Stellenbosch, South Africa.
28. Pio Ribeiro, G., Saldarelli, P., Hong, N., Xiang, B. C. H., Zhang, X. L., Wang, G. P. 2004. First record of three grapevine viruses in the Chinese province of Sinkiang. J. Plant Pathol., 86, 26.
29. Sharma A. M., Wang J., Duffy S., Zhang S., Wong M. K., Rashed A. 2011. Occurrence of grapevine leafroll-associated virus complex in Napa Valley. PLoS ONE 6:e2622710.1371/journal.pone.0026227
30. Teliz, D. 1987. Field serological detection of viral antigens associated with grapevine leafroll disease. Plant Dis. 71, 704–709.
31. Tsai C. W., Bosco D., Daane K. M, Almeida R. P. P. 2011. Effect of host plant tissue on the vector transmission of grapevine leafroll-associated virus 3. J. Econ. Entomol. 104:1480–1485.
32. Ward E., Foster S. J., Fraaije B. A., McCartney H. A. 2004. Plant pathogen diagnostics: immunological and nucleic acid-based approaches. Ann. Appl. Biol. 145, 1–16
33. Zee F., Gonsalves D., Kim K.S., Pool R., Lee R.F., 1987. Cytopathology of leafroll diseases grapevine and the purification and serology of associated closteroviruslike particles. Phytopathology 77: 1427-1434.