Determination of enzyme activities of susceptible and field populations of <em>Helicoverpa armigera </em>(Hubner) (Lepidoptera: Noctuidae) and its relationship with insecticide resistance

Abstract

The purpose of this study was to determine the activities of general esterase, glutathione S-transferase (GST), and acetylcholinesterase (AChE) enzymes of Helicoverpa armigera (Hubner) strains, one of the most important pests of cotton, collected from cotton fields in Adana, Hatay, Antalya and susceptible strain (Israel), and to determine their relationship with insecticide resistance. The activities of general esterase, glutathione S-transferase enzyme (GST) and acetylcholinesterase (AChE) enzymes were determined in Helicoverpa armigera strains.  The activities of general esterase enzyme in Adana, Hatay and Antalya populations were determined 0.65, 0.8 ve 1.14 fold, respectively. Adana and Hatay strains had lower general esterase enzyme activities than that of susceptible strain. However, general esterase enzyme activity in Antalya strain was found to be similar to susceptible strain. GST enzyme activities in Adana, Hatay and Antalya populations were determined 0.75, 0.92 ve 1.06 fold, and AChE enzyme activities were 0.43, 0.60 and 0.80 fold, respectively.  GST and AChE enzyme activities of field strains and susceptible strain were found to be similar. Enzymes activities and insecticide resistance had been compared with the resistance ratios of at the same populations, to determine the relationship between enzyme activities and insecticide resistance in H. armigera.

Keywords

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Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)’nın hassas ve tarla popülasyonlarında enzim aktivitelerinin belirlenmesi ile insektisitlere dayanıklılık arasındaki ilişki

Abstract

Bu çalışma, pamuğun önemli zararlılarından biri olan Helicoverpa armigera (Hübner)'nın İsrail (hassas)'den getirtilen ve Adana, Hatay ve Antalya illerindeki pamuk ekim alanlarından toplanan popülasyonlarda genel esteraz, glutatyon S-transferaz ve asetilkolinesteraz enzim aktivitelerini ve enzim aktiviteleri ile dayanıklılık arasındaki ilişkileri belirlemek amacıyla ele alınmıştır. H. armigera popülasyonlarının genel esteraz, glutatyon S-transferaz (GST) ve asetilkolinesteraz (AChE) enzim aktiviteleri ölçülmüştür. Genel esteraz enzim aktivitesi Adana, Hatay ve Antalya popülasyonlarında hassasa göre sırasıyla 0.65, 0.80 ve 1.14 kat olarak belirlenmiştir. Tarla popülasyonlarının genel esteraz enzim aktiviteleri hassas popülasyona göre Adana ve Hatay popülasyonlarında daha düşük bulunmasına rağmen, Antalya popülasyonunda benzer bulunmuştur.  GST enzim aktivitesi Adana, Hatay ve Antalya popülasyonlarında sırasıyla 0.75, 0.92 ve 1.06 kat, AChE enzim aktivitesi ise Adana Hatay ve Antalya popülasyonlarında sırasıyla 0.43, 0.60 ve 0.80 kat olarak belirlenmiştir. Genel esteraz enzim aktivitesi GST ve AChE enzim aktivitesi açısından tarla popülasyonları ile hassas popülasyon arasındaki farklılık istatistiksel olarak önemli bulunmamıştır. Belirlenen enzim aktiviteleri ve insektisitlere dayanıklılık arasında bir ilişkinin olup olmadığı, aynı popülasyonlarda daha önce belirlenen dayanıklılık oranları ile karşılaştırılmıştır.

Keywords

• Yeşilkurt, Helicoverpa armigera, enzim aktiviteleri, insektisit dayanıklılığı, pamuk

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