Investigation of the effects of some plant extracts and chemical substances on the infection of <em>Tobacco mosaic virus</em>

Abstract

In the present study, the inhibitory effects of 14 plant extracts and 15 chemical substances were examined on the infection of Tobacco mosaic virus (TMV). According to the results of experiments on test plants, plant extracts inhibited virus infection at the ratios of 25.92 % to 97.19 % while the inhibition levels of chemical substances on virus infection ranged between 10.24 % and 98.30 %. The findings showed that the highest inhibition levels were obtained with three plant extracts (Dianthus caryophyllus, Capsicum annuum and Yucca elephantipes) and three chemicals (Sodium dodecylsulfate, Triton X-100 and HuwaSan). Therefore, the mentioned extracts and chemicals were selected for further steps of the study at which the effects of some factors as dilution, temperature and application times on the inhibition of virus infection were examined. Considering the data from assays on test plants, it was observed that inhibitory activity of D. caryophyllus and sodium dodecylsulfate was not affected much more with dilution and temperature fluctuations. Moreover, the results showed that extract from D. caryophyllus had more inhibitory effect when it was applied in 2 and 4 hours before virus inoculation and on the contrary Sodium dodecylsulfate inhibited virus infection at higher level when used in 2 and 4 hours after virus application. Later, in pot experiments performed on tobacco (Nicotiana tabacum L. cv. Akhisar 97) plants, it was observed that the application of D. caryophyllus extract and reduction of virus concentration in the tobacco plants by spreying sodium dodecylsulfate before or after virus inoculation was determined by results of DAS-ELISA test.

Keywords

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Bazı bitki ekstraktları ve kimyasal maddelerin tütün mozaik virüsü enfeksiyonuna etkilerinin araştırılması

Abstract

Keywords

• Tütün, tütün mozaik virüsü, engelleme, bitki ekstraktı, kimyasal madde.

References

1. Apablaza G. E. and Bernier C. C., 1972. Inhibition of Tobacco Mosaic Virus Infection by Plant Extracts. Can. J. Bot. 50 (7): 1473-1478.
2. Bagley C. A. 2001. Controlling Tobacco Mosaic Virus in Tobacco through Resistance. Master of Science in Crop and Soil Environmental Sciences, 76 p.
3. Bawden F. C. 1954. Inhibitors and Plant Viruses. Advances in Virus Research, 2: 31-57.
4. Blaszczak W., Ross A. F. and Larson R. H. 1959. The Inhibitory Activity of Plant Juices on the Infectivity of Potato Virus X. Phytopathology, 49: 784-791.
5. Clark M. F. and Adams A. N. 1977. Characteristics of the Microplate Metod of Enzyme- linked Immunosorbent Assay for the Detection of Plant Viruses. J. Gen. Virology, 34: 475-483.
6. Corbett M. K. and Sisler H. D. 1967. Plant Virology. Univ. Of Florida Press, Gainesville, U.S.A., XVII+527 p.
7. Düzgüneş O., Kesici T., Kavuncu O. ve Gürbüz G. 1987. Araştırma ve Deneme Metodları (İstatistik Metodları-II). Ankara Üniv. Ziraat Fak. Yayınları, No: 1021 (Ders Kitabı: 295), Ankara, 64 s.
8. Erkan S. 1982. Bazı bitki ekstraktları ile Patates X Virüsü (PVX)’nün infeksiyon oluşturma yeteneğinin engellenmesi üzerinde araştırmalar. Doktora Tezi, Ege Üniversitesi, Ziraat Fakültesi, 74 s.

9. Ersan A. 2004. Tütün Sektör Profili, İstanbul Ticaret Odası, Etüt ve Araştırma Şubesi, 23 s.
10. Ginzberg D. and Lomanov A. F. 2006. The Effects of Resistance Stimuli in the Prevention of TMV. http://www2.research.att.com/~kbl/njrsf/ htmlabs06. html#BO (Erişim tarihi: 15.07.2010).
11. Gül U. ve Ataseven Y. 2004. Tütün. Tarımsal Ekonomi Araştırma Enstitüsü, T.E.A.E- Bakış, 6: 11.
12. Hiramatsu A., Kobayashi N. and Osawa N. 1987. Properties of Two Inhibitors of Plant Virus Infection from Fruiting Bodies of Lentinus edodes and from Leaves of Yucca recurvifolia Salisb. Agricultural and Biological Chemistry, 51(3): 897-904.
13. Koprulu O., Alkan B. and Alkan I. 2009. An Overview of Tobacco Economy in the World and in Turkey. Journal of Applied Sciences Research, 5(10): 1780-1784.
14. Ostermann W. D., Meyer U. and Leiser R. M. 1987. Leaf Extract From Carnation Plants (Dianthus caryophyllus L.) as Inducer of Resistance. Zentralbl. Microbiol., 142: 229-238.
15. Panyukov Y. V., Nemykh M. A., Dobrov E. N. and Drachev V. A. 2008. Surfactant- Induced Amorphous Aggregation of Tobacco Mosaic Virus Coat Protein: A Physical Methods Approach. Macromol. Biosci., 8: 199-209.
16. Prasad V., Srivastava S., Verma V. and Verma H. N. 1995. Two Basic Proteins Isolated From Clerodendrum inerme are Inducers of Systemic Antiviral Resistance in Susceptible Plants. Plant Science, 110(1): 73-82.
17. Rafikova E. R., Panyukov Y. V., Arutyunyam A. M., Yahuzhinsky L. S., Drachev V. A. and Dobrov E. N. 2004. Low Sodium Dodecyl Sulfate Concentrations Inhibit Tobacco Mosaic Virus Coat Protein Amorphous Aggregation and Change the Protein Stability. Biohimiâ, 69(12): 1683-1690.
18. Schade C. 1958. Kleines virologisches Praktikum, Pflanzliche Virologie Band I. Einführung in die allgemeinen Probleme, 221-264.
19. Stirpe F., Williams D. G., Onyon L. J. and Legg R. F. 1981. Dianthins, Ribosome- damaging Proteins with Antiviral Properties from Dianthus caryophyllus L. (carnation). Biochem. J., 195:399-405.
20. Türkileri Ş. 1991. Bazı Bitki Ekstraktları İle Domates Mozaik Virüsü (ToMV)’nün İnfeksiyon Oluşturma Yeteneğinin Engellenmesi Üzerinde Araştırmalar. Yüksek Lisans Tezi, Ege Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, 64 s.
21. Watanabe T., Matsuzama Y., Ko K. and Misata T. 1980. Inhibitory Action of Dodecylbenzensulfonate on Tobacco Mosaic Virus Infection. J. Pesticide Sci. 5: 503-509.