Determination and characterization of several virus diseases on almond trees in the Eastern Mediterranean Region

Abstract

Recently, production of almond has tended to increase in the Mediterranean countries as well as in Turkey. As in all plants, almond also suffer from viral diseases leading to qualitative and quantitative losses. Distribution of Prune dwarf virus (PDV), Prunus necrotic ring spot virus (PNRSV), Plum pox virus (PPV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV) and Cherry leaf roll virus (CLRV) diseases on almond trees in the Eastern Mediterranean Region were investigated and its molecular and biological characterization were performed with this study. During the surveys conducted between 2012-2016, 605 almond leaf samples were collected and tested by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) from 5 provinces of the Eastern Mediterranean Region. 169 almond samples (27.93%) of 605 were found to be infected by at least one virus. Of these, 26 of 129 samples (20.16%) in Adana, 90 of 299 samples (30.10%) in Mersin, 11 of 71 samples (15.49%) in Kahramanmaraş, 34 of 89 samples (38.20%) in Hatay and 8 of 17 samples (47.06%) in Osmaniye were found to be infected at least one virus. While PNRSV, PDV, ACLSV and mixed infection were detected at rate 64%, 18%, 11% and 7% respectively, ApMV, PPV, and CLRV were not found in tested samples. Genomic regions containing the coat protein (CP) gene was reproduced by RT-PCR, resulting in bands of 616 bp for PNRSV, 862 bp for PDV and 358 bp for ACLSV. As they are similar in nucleotide level with 97-99% of the world PNRSV isolates registered to NCBI, 87-96% with PDV isolates, and 81-96% with ACLSV isolates of almond, it is considered that these pathogens spread due to the trade of plant materials.

Keywords

• almond,virus,ELISA,PCR

Doğu Akdeniz Bölgesinde badem ağaçlarında görülen bazı virüs hastalıklarının saptanması ve karakterizasyonu

Abstract

Son yıllarda Akdeniz ülkelerinde olduğu gibi ülkemizde de badem üretimi yıldan yıla artış eğilimine girmiştir. Tüm bitkilerde olduğu gibi bademlerde de virüs hastalıklarından dolayı kayıplar meydana gelmektedir. Bu çalışma ile Doğu Akdeniz Bölgesindeki badem alanlarında ekonomik olarak zarar yapan Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Plum pox virus (PPV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV) ve Cherry leaf roll virus (CLRV)’un bölgemizdeki dağılımı araştırılarak, moleküler ve biyolojik karakterizasyonu yapılmıştır. 2012-2016 yılları arasında yapılan sürvey çalışmalarında, Doğu Akdeniz Bölgesindeki 5 ilden toplam 605 adet badem örneği toplanıp DAS-ELISA testine tabi tutulmuştur. Toplanan 605 badem örneğinden 169 tanesi (% 27,93) en az bir virüsle enfekteli bulunmuştur. Bunlardan, Adana ilinde toplanan 129 badem örneğinin 26’sı (%20,16), Mersin’de 299 örneğin 90’ı (%30,10), Kahramanmaraş’ta 71 örneğin 11’i (%15,49), Hatay’da 89 örneğin 34’ü (%38,20) ve Osmaniye’de 17 örneğin 8’i (%47,06) en az bir virüs ile enfekteli bulunmuştur. Test edilen örneklerde PNRSV %64, PDV %18, ACLSV %11 ve karışık enfeksiyon %7 oranında tespit edilmiş, ApMV, PPV ve CLRV ise tespit edilememiştir. Enfekteli badem izolatlarından, kılıf protein genini içeren gen bölgesi RT-PCR ile çoğaltılarak PNRSV için 616 bp, PDV için 862 bp ve ACLSV için 358 bp’lik bantlar elde edilmiştir. Badem izolatlarının NCBI’a kayıtlı dünya PNRSV izolatları ile %97-99, PDV izolatları ile %87-96, ACLSV izolatları ile %81-96 oranında nükleotit düzeyde benzerlik gösterdiğinden bu etmenlerin bitki materyallerinin ticaretinden dolayı yayıldığı düşünülmektedir.

Keywords

• badem,virüs,ELISA,PCR

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