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Bazı bitki fungal patojenlerinde dsRNA mikovirüslerinin varlığının taranması

Year 2020, , 57 - 62, 31.03.2020
https://doi.org/10.16955/bitkorb.612275

Abstract



Mikovirüsler birçok fungal hastalık etmenini enfekte edebilir ve bunların bazıları hipovirülensliğe neden olabilmektedir. Bu mikovirüsler bitkilerin biyolojik mücadelesinde yaygın olarak kullanılmaktadırlar. Mikovirüslerin kullanılması ile gerçekleştirilen biyolojik mücadeleye en başarılı örnek, Kestane kanseri hastalığıdır. Bu çalışmada, asmadan elde edilen Phomopsis viticola, pamuktan ve zeytinden Verticillium dahliae, pamuktan Rhizoctonia solani ve kirazdan Leucostoma spp., izolatlarındaki mikovirüslerin varlığı dsRNA metodu ile araştırılmıştır. dsRNA ekstraksiyonu yapılmış ve agaroz jel elektroforez yöntemi ile analiz edilmiştir. Asmada 80 P. viticola izolatının 8’inde, zeytinde 50 V. dahlia izolatının 1’inde, pamukta 50 V. dahliae izolatının 3’ünde, pamukta 50 R. solani izolatının 6’sında ve kirazda 50 Leucostoma spp. izolatının 3’ünde dsRNA bantları elde edilmiştir. Elde edilen dsRNA bantlarının tahmini moleküler ağırlıkları 12-20 kb arasında bulunmuştur.



Supporting Institution

Adenan Menderes Üniversitesi Bilimsel araştırma proje

Project Number

ZRF16009

References

  • Abbas A., 2016. A Review Paper on Mycoviruses. Journal of Plant Pathol Microbiol. 7-12.
  • Balijja A., Kvarnheden A., Turchetti T., 2008. A non-phenol–chloroform extraction of double-stranded RNA from plant and fungal tissues. Journal of Virological Methods. 152:32–37.
  • Bharathan N., Saso H., Gudipati L., Bharathan S., Whited K., 2005. Double-stranded RNA: distribution and analysis among isolates of Rhizoctonia solani AG-2 to -1. Plant Pathol. 54:196–203.
  • Bharathan N., Tavantzis SM., 1991. Assessment of genetic relatedness among doublestranded RNAs from isolates of Rhizoctonia solani from diverse geographic origins. Phytopathology 81:411-5.
  • Bharathan N., Tavantzis S. M., 1990. Genetic diversity of double-stranded RNA from Rhizoctonia solani. Phytopathology 80:631-5.
  • Canızares E., Perez-artes M., García-pedrajas N., Garcia-pedrajas M., 2015. Characterization of a new partitivirus strain in Verticillium dahliae provides further evidence of the spread of the highly virulent defoliating pathotype through new introductions. Phytopathologia Mediterranea. 54(3):516−523.
  • Cao Y., Zhu Xw., Xiang Y., Li D. q., Yang J. r., Mao Q. z., Chen J. s., 2011. Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae’ Kleb. Virus Research. 159:73–78.
  • Das S., Falloon R. E., Stewart A., Pitman A. R., 2014. Molecular characterisation of an endornavirus from Rhizoctonia solani AG-3PT infecting potato. Fungal Biol. 118: 924–934.
  • Feng Z., Zhu H., Li Z., Shi Y., Zhao L., Liu L., Jiang D., 2013. Complete genome sequence of a novel dsRNA mycovirus isolated from the phytopathogenic fungus Verticillium dahliae Kleb. Arch Virol. 158:2621– 2623.
  • Hyakumachi M., Sumino A., Veda I., Shikata E., 1985. Relationship between the presence of dsRNA in Rhizoctonia solani and pathogenicity. Annals of the Phytopathological Society of Japan. 51:372–3.
  • Jensen C. J. P., Adams G. C., 1995. Nitrogen Metabolism of Leucostoma persoonii and L.cincta in Virulent and Hypovirulent Isolates. Mycologia. 87(6):864-875.
  • Jiang D., Fu Y., Ghabrial S. A., 2013. Mycoviruses: Chapter eight—Viruses of the plant pathogenic fungus Sclerotinia sclerotiorum. Adv Virus Res. 86:215–248.
  • Koloniuk I., El-Habbak M. H., Petrzik K., Ghabrial S. A., 2014. Complete genome sequence of a novel hypovirus infecting Phomopsis longicolla. Arch Virol. 159:1861–1863.
  • Kousik C.S., Snow J. P., Valrerde R. P., 1993. Comparison of Double Stranded RNA Componts and Virulence Among Isolates of Rhizoctonia solani AG_1 IA and AG_1 IB. Phytopathology. 84:44-49.
  • Preisig O., Moleleki N., Smit W. A., Wingfield B. D., Wingfield M. J., 2000. A novel RNA mycovirus in a hypovirulent isolate of the plant pathogen Diaporthe ambigua. Journal of General Virology. 81:3107–3114.
  • Son M., Yu1 J., Kim K., 2015. Five Questions about Mycovirusesi. PLOS Pathogens DOI:10.1371/journal.ppat.1005172 November. 5:2015.
  • Tonguslu M., Acıkgoz S., 2015. Investigation of virulence and presence of mikoviral dsRNA on leucostoma spp. isolates of the cherry production areas in the aegean region/turkey. International ScientificAgriculture Symposium "Agrosym 2015" Jahorina, 15-18 October 2015, Bosnia and Herzegovina.
  • Van Regenmortel M. H., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., aniloff J., Mayo M. A., Mcgeoch D. J., Pringle C. R., Wickner R. B., 2000. Virus Taxonomy, Classification and Nomenclature of Viruses, Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press. London, UK.
  • Xie J., Jiang D., 2014. New Insights into Mycoviruses and Exploration for the Biological Control of Crop Fungal Diseases. Annu. Rev. Phytopathol. 52:45–68.
  • Zanzinger D. H., Bandy B. P., Tavantzis, S.M., 1984. High frequency of finding double-stranded RNA in naturally occurring isolates of Rhizoctonia solani. Journal of General Virology. 65:1601-5.
  • Zhang R. J., Zhong J., Shang H. H., Pan X. T., Zhu H. J., Gao B. D., 2015. The complete nucleotide sequence and genomic organization of a novel victorivirus with two non-overlapping ORFs, identified in the plant-pathogenic fungus Phomopsis vexans. Arch Virol. 160:1805–1809.

Screening of some plant pathogenic fungi for the presence of dsRNA mycoviruses

Year 2020, , 57 - 62, 31.03.2020
https://doi.org/10.16955/bitkorb.612275

Abstract

Mycoviruses can infect many fungi and some may cause hypovirulence, which is a common phenomenon used in the biological control of certain fungal diseases of the plants. The most successful example regarding the use of mycoviruses and hypovirulent strains in biological control is the chestnut blight. In this study, the isolates of fungi, namely Phomopsis viticola from grapevine, Verticillium dahliae from cotton and olive, Rhizoctonia solani from cotton and Leucostoma spp., from cherry were screened for the presence of dsRNA mycoviruses. Isolation of dsRNAs was performed and dsRNA bands were analyzed by agarose gel electrophoresis. Putative dsRNA bands were detected in eight of 80 P. viticola isolates from grapevine, one of 50 V. dahliae isolates from olive, three of 50 V. dahliae isolates from cotton, six of 50 R. solani isolates from cotton and three of 50 Leucostoma spp., isolates from cherry. The estimated molecular sizes of the dsRNAs ranged from approximately 12.0 to 20.0 kb.

Project Number

ZRF16009

References

  • Abbas A., 2016. A Review Paper on Mycoviruses. Journal of Plant Pathol Microbiol. 7-12.
  • Balijja A., Kvarnheden A., Turchetti T., 2008. A non-phenol–chloroform extraction of double-stranded RNA from plant and fungal tissues. Journal of Virological Methods. 152:32–37.
  • Bharathan N., Saso H., Gudipati L., Bharathan S., Whited K., 2005. Double-stranded RNA: distribution and analysis among isolates of Rhizoctonia solani AG-2 to -1. Plant Pathol. 54:196–203.
  • Bharathan N., Tavantzis SM., 1991. Assessment of genetic relatedness among doublestranded RNAs from isolates of Rhizoctonia solani from diverse geographic origins. Phytopathology 81:411-5.
  • Bharathan N., Tavantzis S. M., 1990. Genetic diversity of double-stranded RNA from Rhizoctonia solani. Phytopathology 80:631-5.
  • Canızares E., Perez-artes M., García-pedrajas N., Garcia-pedrajas M., 2015. Characterization of a new partitivirus strain in Verticillium dahliae provides further evidence of the spread of the highly virulent defoliating pathotype through new introductions. Phytopathologia Mediterranea. 54(3):516−523.
  • Cao Y., Zhu Xw., Xiang Y., Li D. q., Yang J. r., Mao Q. z., Chen J. s., 2011. Genomic characterization of a novel dsRNA virus detected in the phytopathogenic fungus Verticillium dahliae’ Kleb. Virus Research. 159:73–78.
  • Das S., Falloon R. E., Stewart A., Pitman A. R., 2014. Molecular characterisation of an endornavirus from Rhizoctonia solani AG-3PT infecting potato. Fungal Biol. 118: 924–934.
  • Feng Z., Zhu H., Li Z., Shi Y., Zhao L., Liu L., Jiang D., 2013. Complete genome sequence of a novel dsRNA mycovirus isolated from the phytopathogenic fungus Verticillium dahliae Kleb. Arch Virol. 158:2621– 2623.
  • Hyakumachi M., Sumino A., Veda I., Shikata E., 1985. Relationship between the presence of dsRNA in Rhizoctonia solani and pathogenicity. Annals of the Phytopathological Society of Japan. 51:372–3.
  • Jensen C. J. P., Adams G. C., 1995. Nitrogen Metabolism of Leucostoma persoonii and L.cincta in Virulent and Hypovirulent Isolates. Mycologia. 87(6):864-875.
  • Jiang D., Fu Y., Ghabrial S. A., 2013. Mycoviruses: Chapter eight—Viruses of the plant pathogenic fungus Sclerotinia sclerotiorum. Adv Virus Res. 86:215–248.
  • Koloniuk I., El-Habbak M. H., Petrzik K., Ghabrial S. A., 2014. Complete genome sequence of a novel hypovirus infecting Phomopsis longicolla. Arch Virol. 159:1861–1863.
  • Kousik C.S., Snow J. P., Valrerde R. P., 1993. Comparison of Double Stranded RNA Componts and Virulence Among Isolates of Rhizoctonia solani AG_1 IA and AG_1 IB. Phytopathology. 84:44-49.
  • Preisig O., Moleleki N., Smit W. A., Wingfield B. D., Wingfield M. J., 2000. A novel RNA mycovirus in a hypovirulent isolate of the plant pathogen Diaporthe ambigua. Journal of General Virology. 81:3107–3114.
  • Son M., Yu1 J., Kim K., 2015. Five Questions about Mycovirusesi. PLOS Pathogens DOI:10.1371/journal.ppat.1005172 November. 5:2015.
  • Tonguslu M., Acıkgoz S., 2015. Investigation of virulence and presence of mikoviral dsRNA on leucostoma spp. isolates of the cherry production areas in the aegean region/turkey. International ScientificAgriculture Symposium "Agrosym 2015" Jahorina, 15-18 October 2015, Bosnia and Herzegovina.
  • Van Regenmortel M. H., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., aniloff J., Mayo M. A., Mcgeoch D. J., Pringle C. R., Wickner R. B., 2000. Virus Taxonomy, Classification and Nomenclature of Viruses, Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press. London, UK.
  • Xie J., Jiang D., 2014. New Insights into Mycoviruses and Exploration for the Biological Control of Crop Fungal Diseases. Annu. Rev. Phytopathol. 52:45–68.
  • Zanzinger D. H., Bandy B. P., Tavantzis, S.M., 1984. High frequency of finding double-stranded RNA in naturally occurring isolates of Rhizoctonia solani. Journal of General Virology. 65:1601-5.
  • Zhang R. J., Zhong J., Shang H. H., Pan X. T., Zhu H. J., Gao B. D., 2015. The complete nucleotide sequence and genomic organization of a novel victorivirus with two non-overlapping ORFs, identified in the plant-pathogenic fungus Phomopsis vexans. Arch Virol. 160:1805–1809.
There are 21 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Sahra Hosseinalizadeh 0000-0002-7491-242X

Ömer Erincik 0000-0002-0304-7475

Serap Açıkgöz 0000-0002-7970-1648

Project Number ZRF16009
Publication Date March 31, 2020
Submission Date August 28, 2019
Acceptance Date December 7, 2019
Published in Issue Year 2020

Cite

APA Hosseinalizadeh, S., Erincik, Ö., & Açıkgöz, S. (2020). Screening of some plant pathogenic fungi for the presence of dsRNA mycoviruses. Plant Protection Bulletin, 60(1), 57-62. https://doi.org/10.16955/bitkorb.612275
AMA Hosseinalizadeh S, Erincik Ö, Açıkgöz S. Screening of some plant pathogenic fungi for the presence of dsRNA mycoviruses. Plant Protection Bulletin. March 2020;60(1):57-62. doi:10.16955/bitkorb.612275
Chicago Hosseinalizadeh, Sahra, Ömer Erincik, and Serap Açıkgöz. “Screening of Some Plant Pathogenic Fungi for the Presence of DsRNA Mycoviruses”. Plant Protection Bulletin 60, no. 1 (March 2020): 57-62. https://doi.org/10.16955/bitkorb.612275.
EndNote Hosseinalizadeh S, Erincik Ö, Açıkgöz S (March 1, 2020) Screening of some plant pathogenic fungi for the presence of dsRNA mycoviruses. Plant Protection Bulletin 60 1 57–62.
IEEE S. Hosseinalizadeh, Ö. Erincik, and S. Açıkgöz, “Screening of some plant pathogenic fungi for the presence of dsRNA mycoviruses”, Plant Protection Bulletin, vol. 60, no. 1, pp. 57–62, 2020, doi: 10.16955/bitkorb.612275.
ISNAD Hosseinalizadeh, Sahra et al. “Screening of Some Plant Pathogenic Fungi for the Presence of DsRNA Mycoviruses”. Plant Protection Bulletin 60/1 (March 2020), 57-62. https://doi.org/10.16955/bitkorb.612275.
JAMA Hosseinalizadeh S, Erincik Ö, Açıkgöz S. Screening of some plant pathogenic fungi for the presence of dsRNA mycoviruses. Plant Protection Bulletin. 2020;60:57–62.
MLA Hosseinalizadeh, Sahra et al. “Screening of Some Plant Pathogenic Fungi for the Presence of DsRNA Mycoviruses”. Plant Protection Bulletin, vol. 60, no. 1, 2020, pp. 57-62, doi:10.16955/bitkorb.612275.
Vancouver Hosseinalizadeh S, Erincik Ö, Açıkgöz S. Screening of some plant pathogenic fungi for the presence of dsRNA mycoviruses. Plant Protection Bulletin. 2020;60(1):57-62.

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