Tokat ilinde biber alanlarında Tomato spotted wilt virus’ın yaygınlığı ve moleküler karakterizasyonu

Öz

Çalışma kapsamında 2016 yılı yaz döneminde biber yetiştirilen Tokat ili Merkez, Niksar, Erbaa ve Pazar ilçelerine sürveyler düzenlenmiş ve virüs şüphesi gösteren biber bitkilerinden yaprak örneği toplanmıştır. Sürvey çalışmaları sonucunda toplanan 324 adet bitki yaprak örneği Tomato spotted wilt virus (TSWV)’e spesifik antiserum ile DAS-ELISA testine tabii tutulduktan sonra virüse spesifik primerlerle RT-PCR işlemi gerçekleştirilmiştir. Testlenen örneklerin %13’nün TSWV ile enfekteli olduğu tespit edilmiştir. ELISA testinde pozitif çıkan örnekler S segmentinde yer alan Nucleocapsid proteine (Np) spesifik primerlerle RT-PCR işlemine tabi tutulmuş ve pozitif bant elde edilen örneklerden 3’ü sekans analizine gönderilmiştir. Analiz sonuçlarına göre Türkiye TSWV izolatlarının Np gen bölgesi Fransa ve Güney Kore izolatları ile %98-99 nükleotid benzerliği göstermiştir ve filogenetik ağaçta aynı grupta kümelenmiştir.Anahtar kelimeler: biber, RT-PCR, Tomato spotted wilt virus, Tokat

Anahtar Kelimeler

• biber
• RT-PCR
• Tomato spotted wilt virus
• Tokat

Prevalence and molecular characterization of Tomato spotted wilt virus in pepper fields in Tokat province

Abstract

The study was conducted in Tokat Center, Niksar, Erbaa and Pazar districts where peppers were grown in the summer of 2016, and leaf samples were collected from plants suspected of the virus. During the surveys, a total of 324 plant samples were collected and the infected pepper samples were subjected to DAS-ELISA test with (Tomato spotted wilt virus) TSWV-specific antiserum, and RT-PCR was performed with virus-specific primers. In DAS-ELISA studies, 324 plants were tested and 13% of the samples were found to be TSWV infected. Samples that were positive in ELISA test were subjected to RT-PCR with nucleocapsid gene specific primers in the S segment and three samples were sent for sequence analysis. According to results, Turkey TSWV isolates Np gene region have shown 98-99% nucleotide identity with the isolates from France and South Korea and grouped with them same group.

Keywords

• pepper
• RT-PCR
• TSWV
• Tokat

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