Molecular diversity of ‘Candidatus Phytoplasma’ species in pome and stone fruits in Turkey

Year 2019, Volume: 59 Issue: 1, 7 - 14, 29.03.2019

Didem Canik Orel , Samanta Paltrınıerı Filiz Ertunç , Assunta Bertaccını

https://doi.org/10.16955/bitkorb.508890

Cited By: 7

Abstract

Apple, pear and apricot trees showing phytoplasma
associated symptoms from Ankara and Isparta provinces were sampled and
investigated to verify the presence of phytoplasma-associated diseases. Totally
31 samples were tested with phytoplasma universal and group specific primers
and the PCR products were restricted using Tru1I,
RsaI and SspI endonucleases. Different RFLP profiles were obtained and
selected samples were directly sequenced. The apple samples were found infected
with 16SrX-A (‘Candidatus Phytoplasma
mali’), while the majority of the pear samples were infected with 16SrX-A and
16SrX-C subgroup phytoplasmas in mixed infection. The 16SrX-B (‘Candidatus Phytoplasma prunorum’),
16SrX-C (‘Candidatus Phytoplasma
pyri’) and mixed infection of 16SrX-A/16SrX-C and 16SrX-C/16SrI (aster yellows)
were detected in the apricot samples. In this study the 16SrI phytoplasmas in
apricot and the mixed phytoplasma infections in pear and apricot trees were detected
in Turkey for the first time.

Keywords

Phytoplasma detection, Pome fruits, Apricot, Mixed phytoplasma infection, Aster yellows group

Türkiye’de yumuşak ve sert çekirdekli meyvelerde ‘Candidatus Phytoplasma’ türlerinin moleküler çeşitliliği

Abstract

Ankara ve Isparta illerinden fitoplazma enfeksiyonu
benzeri belirti gösteren elma, armut ve kayısı ağaçlarından fitoplazma varlığının
saptanmasına yönelik örnekleme yapılmıştır. Toplamda 31 örnek universal ve grup
spesifik fitoplazma primerleri ile PCR’a tabi tutulmuş ve elde edilen PCR
ürünleri Tru1I, RsaI and SspI
endonükleazları ile kesilmiştir. Elma örnekleri 16SrX-A (‘Candidatus Phytoplasma mali’) ile enfekteli bulunurken armut
örneklerinin çoğunluğunda 16SrX-A ve 16SrX-C alt gruplarıyla karışık enfeksiyon
saptanmıştır. Kayısı örneklerinde 16SrX-B (‘Candidatus
Phytoplasma prunorum’), 16SrX-C (‘Candidatus
Phytoplasma pyri’) ve 16SrX-A/16SrX-C ve 16SrX-C/16SrI (aster yellows) alt gruplarının
karışık enfeksiyonu saptanmıştır. Bu çalışma ile kayısıda 16SrI, armutta ve
kayısıda karışık fitoplazma enfeksiyonları Türkiye’de ilk kez saptanmıştır. 

Keywords

Fitoplazma tespiti, Yumuşak çekirdekliler, Kayısı, Karışık fitoplazma enfeksiyonu, Aster yellows grup

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